Development of a 10 T Cryocooled Superconducting Magnet with a Room Temperature Bore of 360 mm for a 29 T Hybrid Magnet

Ito, Tomoyuki; Ishizuka, Masato; Tsurudome, Takehisa; Ookubo, Hiroshi; Sakuraba, J.; Awaji, Satoshi; Nishijima, G.; Koyama, K.; Takahashi, Keita; Watanabe, Kenji

doi:10.1063/1.2202602

Cited by 4 publications

(4 citation statements)

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“…RPL22 and GAPDH interact specifically with the 3Ј NTR (38). PTBP1, ELAVL1, and HNRNPC bind to the 3Ј termini of both (ϩ) and (Ϫ) strands, suggesting they may be components of the RNA synthesis initiation complexes (39,40).…”

Section: Discussionmentioning

confidence: 99%

Cellular cofactors affecting hepatitis C virus infection and replication

Randall

Panis²,

Cooper

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

496

454

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Recently identified hepatitis C virus (HCV) isolates that are infectious in cell culture provide a genetic system to evaluate the significance of virus-host interactions for HCV replication. We have completed a systematic RNAi screen wherein siRNAs were designed that target 62 host genes encoding proteins that physically interact with HCV RNA or proteins or belong to cellular pathways thought to modulate HCV infection. This includes 10 host proteins that we identify in this study to bind HCV NS5A. siRNAs that target 26 of these host genes alter infectious HCV production >3-fold. Included in this set of 26 were siRNAs that target Dicer, a principal component of the RNAi silencing pathway. Contrary to the hypothesis that RNAi is an antiviral pathway in mammals, as has been reported for subgenomic HCV replicons, siRNAs that target Dicer inhibited HCV replication. Furthermore, siRNAs that target several other components of the RNAi pathway also inhibit HCV replication. MicroRNA profiling of human liver, human hepatoma Huh-7.5 cells, and Huh-7.5 cells that harbor replicating HCV demonstrated that miR-122 is the predominant microRNA in each environment. miR-122 has been previously implicated in positively regulating the replication of HCV genotype 1 replicons. We find that 2-O-methyl antisense oligonucleotide depletion of miR-122 also inhibits HCV genotype 2a replication and infectious virus production. Our data define 26 host genes that modulate HCV infection and indicate that the requirement for functional RNAi for HCV replication is dominant over any antiviral activity this pathway may exert against HCV.antivirals ͉ miR-122 ͉ RNAi ͉ HCVcc-siRNA H epatitis C virus (HCV) has a notable ability to establish persistent infections in Ϸ70% of cases, resulting in 130 million chronically infected people throughout the world (1). This prevalence has spurred considerable interest in the study of HCV-host interactions, on both cellular and molecular levels. The inability to grow HCV in cell culture led some groups to focus on the identification of cellular proteins that interact with individual HCV proteins or RNA elements, resulting in the accumulation of a large number of putative HCV-host interactions. Unfortunately, the significance of most of these with respect to the HCV life cycle is currently unknown (reviewed in ref.2). Over the past 6 years, cell culture systems have been developed that enable the characterization of HCV replication and entry (3-6). This effort recently culminated in the development of cell culture systems that reproduce the entire viral life cycle (7-9). A number of virus-host interactions have been characterized by using these experimental systems. For example, CD81 has been demonstrated to play a role in HCV entry (10-12).Sequence-specific gene silencing of RNAi is ideal for assessing the genetic phenotypes associated with virus-host interactions. We have previously shown that siRNAs are highly effective at silencing either host or viral RNAs in cells that contain replicating HCV, demonstrating th...

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Section: Discussionmentioning

confidence: 99%

Cellular cofactors affecting hepatitis C virus infection and replication

Randall

Panis²,

Cooper

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

496

454

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“…15 Ito et al reported the WAXD/SAXS data of biodegradable polymers measured with the taper optics fiber CCD detector as a detector for WAXD. 20 However, these systems were optional at each beamline. Therefore, it was an indispensably important theme for us to supply the rapidlyscanning 2D-WAXD/SAXS measurement system for the common use.…”

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confidence: 99%

“…In SPring-8 and Photon Factory, the WAXD/SAXS simultaneous measurements were carried our by the limited numbers of technically-skilled particular researchers. [13][14][15][16][17]20,21 As mentioned above, the 2D-WAXD/ SAXS measurement system is a necessary tool for kinetic investigations on the hierarchical structure of polymers.…”

mentioning

confidence: 99%

“…The combination of the ultra-bright X-ray beams with highlysensitive detectors makes it possible to perform the above-mentioned in situ WAXD/SAXS simultaneous measurements satisfactorily. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] In the European Synchrotron Radiation Facility (ESRF), 6 The Advance Photon Source (APS), 7,8 and the other synchrotron radiation facilities, for example, the WAXD/SAXS simultaneous measurement system with two-dimensional (2D) detectors (2D-WAXD/SAXS measurement system) is a default utilized for many general users, helping them to create many papers on the structural evolution process of polymers. In SPring-8 and Photon Factory, the WAXD/SAXS simultaneous measurements were carried our by the limited numbers of technically-skilled particular researchers.…”

mentioning

confidence: 99%

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Development of Synchrotron DSC/WAXD/SAXS Simultaneous Measurement System for Polymeric Materials at the BL40B2 in SPring-8 and its Application to the Study of Crystal Phase Transitions of Fluorine Polymers

Masunaga

Sasaki

Tashiro

et al. 2007

Polym J

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ABSTRACT:A new system of rapidly-scanning DSC/WAXD/SAXS simultaneous measurements has been successfully developed at the BL40B2 in SPring-8, Japan. The combination of the ultra-bright and highly-directional X-ray beams with the highly-sensitive two-dimensional (2D) detectors such as an imaging plate (IP), a CCD detector and a flat panel (FP) detector has made it possible to perform the rapidly-scanning time-resolved measurements of WAXD and SAXS for the polymeric materials subjected to the various kinds of external field like temperature, tensile force, magnetic field, hydrostatic pressure, and so on. The experimental data about the phase transitions of vinylidene fluoridetrifluoroethylene copolymer with VDF 82 mol % content and poly(vinylidene fluoride) form I have been presented as actual examples, where the phase transitions of the polar crystal consisting of planar-zigzag chains to the gauche-type high-temperature phase have been confirmed definitely and the remarkable change in lamellar stacking structure could be detected in the temperature region immediately below the melting point. [doi:10.1295/polymj.PJ2007105] KEY WORDS Simultaneous Measurement System / Wide-angle X-ray Diffraction / Small-angle X-ray Scattering / Differential Scanning Calorimetry / SPring-8 / Poly(vinylidene fluoride) / Phase Transition / Polymeric materials consist of complicated aggregation state of chains. In the case of semicrystalline polymer, for example, these chains gather together to form the crystalline region or a crystalline lamella. These lamellae are stacked together to construct a spherulite. There are amorphous region between these stacked lamellae. In the spherulite, the lamellae extend and twist periodically from the spherulitic center along the radial direction, giving the so-called extinction ring in its polarized optical microscopic image. It is essentially important to clarify the evolution process of this complicated hierarchical structure of polymers from the various viewpoints on angstrom-tomicrometer scale in order to design and control their physical and structural properties. One of the most useful and quantitative methods for attainment of this purpose is to use the X-ray scattering technique. The wide-angle X-ray diffraction (WAXD) gives us information on the chain conformation and the aggregation state of chains in the crystalline and amorphous regions. The small-angle X-ray scattering (SAXS) tells us about the rough form of molecular chains, the aggregation structure of crystalline lamellae, and so on. Therefore, the time-resolved measurements of WAXD and SAXS allow us to trace the structural evolution process of polymers in the heating or cooling process, for example. There were many reports on the WAXD and/or SAXS experimental data.1 In most cases the WAXD or SAXS measurement was performed for a sample separately by assuming the reproducibility of the experimental conditions. But, strictly speaking, we do not have any guarantee about the reproducibility of the experimental conditions in the WAXD and S...

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