Development of 3-D poly(trimethylenecarbonate-co-ε-caprolactone)-block-poly(p-dioxanone) scaffold for bone regeneration with high porosity using a wet electrospinning method

Shin, Teo Jeon; Park, Sook Young; Kim, Hyun Jeong; Lee, Hak Jun; Youk, Ji Ho

doi:10.1007/s10529-010-0235-7

Cited by 29 publications

(16 citation statements)

References 13 publications

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“…Meanwhile, the seeded cells were thin and narrow in shape, and adhered to a relatively small area of the scaffold. 40 Subsequently, MSCs started to migrate through the pores and integrated well with the surrounding fibers on day 3 ( Figure 2C and D). Moreover, it …”

Section: Morphology Analysis Of the Electrospun Peg/pla Fibrous Scaffmentioning

confidence: 93%

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Fu²,

Fan³

et al. 2011

IJN

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Polylactide (PLA) electrospun fibers have been reported as a scaffold for bone tissue engineering application, however, the great hydrophobicity limits its broad application. In this study, the hybrid amphiphilic poly(ethylene glycol) (PEG)/hydrophobic PLA fibrous scaffolds exhibited improved morphology with regular and continuous fibers compared to corresponding blank PLA fiber mats. The prepared PEG/PLA fibrous scaffolds favored mesenchymal stem cell (MSC) attachment and proliferation by providing an interconnected porous extracellular environment. Meanwhile, MSCs can penetrate into the fibrous scaffold through the interstitial pores and integrate well with the surrounding fibers, which is very important for favorable application in tissue engineering. More importantly, the electrospun hybrid PEG/PLA fibrous scaffolds can enhance MSCs to differentiate into bone-associated cells by comprehensively evaluating the representative markers of the osteogenic procedure with messenger ribonucleic acid quantitation and protein analysis. MSCs on the PEG/PLA fibrous scaffolds presented better differentiation potential with higher messenger ribonucleic acid expression of the earliest osteogenic marker Cbfa-1 and mid-stage osteogenic marker Col I . The significantly higher alkaline phosphatase activity of the PEG/PLA fibrous scaffolds indicated that these can enhance the differentiation of MSCs into osteoblast-like cells. Furthermore, the higher messenger ribonucleic acid level of the late osteogenic differentiation markers OCN ( osteocalcin ) and OPN ( osteopontin ), accompanied by the positive Alizarin red S staining, showed better maturation of osteogenic induction on the PEG/PLA fibrous scaffolds at the mineralization stage of differentiation. After transplantation into the thigh muscle pouches of rats, and evaluating the inflammatory cells surrounding the scaffolds and the physiological characteristics of the surrounding tissues, the PEG/PLA scaffolds presented good biocompatibility. Based on the good cellular response and excellent osteogenic potential in vitro, as well as the biocompatibility with the surrounding tissues in vivo, the electrospun PEG/PLA fibrous scaffolds could be one of the most promising candidates in bone tissue engineering.

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Section: Morphology Analysis Of the Electrospun Peg/pla Fibrous Scaffmentioning

confidence: 93%

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Fu²,

Fan³

et al. 2011

IJN

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“…The main application of such bulky materials is predominant in tissue engineering. Materials produced by wet-electrospinning are poly(glycolic acid) [5], chitin [6], cellulose [7], poly(trimethylenecarbonate-co-!-caprolactone)-block-poly-(p-dioxanone) [8], copolymer of poly(glycolic acid) and poly(lactic acid) and blend of polycaprolactone and collagen or polycaprolactone and elastine [9]. A detailed study devoted to the immersion precipitation process in the electrospinning of polyacrylonitrile (PAN) dissolved in N,N-dimethyl-formamide (DMF) was developed by Fang et al [10].…”

Section: Introductionmentioning

confidence: 99%

Study of polycaprolactone wet electrospinning process

Košťáková¹,

Seps²,

Pokorný³

et al. 2014

Express Polym. Lett.

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Abstract. Wet electrospinning is a useful method for 3-dimensional structure control of nanofibrous materials. This innovative technology uses a liquid collector instead of the metal one commonly used for standard electrospinning. The article compares the internal structural features of polycaprolactone (PCL) nanofibrous materials prepared by both technologies. We analyze the influence of different water/ethanol compositions used as a liquid collector on the morphology of the resultant polycaprolactone nanofibrous materials. Scanning electron micro-photographs have revealed a bimodal structure in the wet electrospun materials composed of micro and nanofibers uniformly distributed across the sample bulk. We have shown that the full-faced, twofold fiber distribution is due to the solvent composition and is induced and enhanced by increasing the ethanol weight ratio. Moreover, the comparison of fibrous layers morphology obtained by wet and dry spinning have revealed that beads that frequently appeared in dry spun materials are created by Plateau-Rayleigh instability of the fraction of thicker fibers. Theoretical conditions for spontaneous and complete immersion of cylindrical fibers into a liquid collector are also derived here.

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“…Because of these considerations, more and more research has been carried out to modify the surface characteristics of micro-scale and submicron scaffold fibers. Methods such as co-spinning finer fibers onto thicker support fibers and the incorporation of inherently porous materials have been considered, but an emerging fabrication method that has begun to gain popularity is wet electrospinning [15,16]. The process of wet electrospinning basically entails a standard electrospinning setup augmented with the collector plate submerged in a methanol bath.…”

Section: Introductionmentioning

confidence: 99%

Enhanced human bone marrow mesenchymal stem cell functions in novel 3D cartilage scaffolds with hydrogen treated multi-walled carbon nanotubes

Holmes

Castro

et al. 2013

Nanotechnology

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Cartilage tissue is a nanostructured tissue which is notoriously hard to regenerate due to its extremely poor inherent regenerative capacity and complex stratified architecture. Current treatment methods are highly invasive and may have many complications. Thus, the goal of this work is to use nanomaterials and nano/microfabrication methods to create novel biologically inspired tissue engineered cartilage scaffolds to facilitate human bone marrow mesenchymal stem cell (MSC) chondrogenesis. To this end we utilized electrospinning to design and fabricate a series of novel 3D biomimetic nanostructured scaffolds based on hydrogen (H2) treated multi-walled carbon nanotubes (MWCNTs) and biocompatible poly(L-lactic acid) (PLLA) polymers. Specifically, a series of electrospun fibrous PLLA scaffolds with controlled fiber dimension were fabricated in this study. In vitro MSC studies showed that stem cells prefer to attach in the scaffolds with smaller fiber diameter. More importantly, the MWCNT embedded scaffolds showed a drastic increase in mechanical strength and a compressive Young's modulus matching to natural cartilage. Furthermore, our MSC differentiation results demonstrated that incorporation of the H2 treated carbon nanotubes and poly-L-lysine coating can induce more chondrogenic differentiations of MSCs than controls. After two weeks of culture, PLLA scaffolds with H2 treated MWCNTs and poly-L-lysine can achieve the highest glycosaminoglycan synthesis, making them promising for further exploration for cartilage regeneration.

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Development of 3-D poly(trimethylenecarbonate-co-ε-caprolactone)-block-poly(p-dioxanone) scaffold for bone regeneration with high porosity using a wet electrospinning method

Cited by 29 publications

References 13 publications

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Study of polycaprolactone wet electrospinning process

Enhanced human bone marrow mesenchymal stem cell functions in novel 3D cartilage scaffolds with hydrogen treated multi-walled carbon nanotubes

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