Development of 23 polymorphic microsatellite loci in invasive silver wattle, Acacia dealbata (Fabaceae)

Guillemaud, Thomas; Broadhurst, Linda; Legoff, Isabelle; Henery, Martin L.; Blin, Aurélie; Ducatillion, Catherine; Ferrando, Nathalie; Malausa, Thibaut

doi:10.3732/apps.1500018

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…Furthermore, several SSRs markers have been developed for Acacia species, such as A. atkinsiana (Levy et al, 2014), A. brevispica (Otero-Arnaiz, 2005. A. dealbata (Guillemaud et al, 2015), Acacia (Senegalia) mellifera (Ruiz-Guajardo et al, 2007), A. nilotica (Wardill et al, 2004), A. mangium (Butcher et al, 2000), A. senegal (also known as Senegalia senegal) (Assoumane et al, 2009). However, to the best of our knowledge, SSR primers have not yet been described for A. longifolia.…”

Section: Introductionmentioning

confidence: 87%

Genetic diversity and differentiation of invasive Acacia longifolia in Portugal

2018

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Abstract. Acacia longifolia is an aggressive invader worldwide. This species was brought to Portugal by the forestry services and is now found throughout the country with very strong ecological and social impacts. Although several ecological and physiological studies have been carried out, molecular studies in this species are sparse. Particularly, genetic variability evaluation in invasive ranges clearly deserves more attention. The aim of this study was to evaluate the genetic diversity and the genetic differentiation among populations of the alien invasive A. longifolia under different Mediterranean conditions. For that we studied three sandy dune populations along the Portuguese coast: Osso da Baleia (mesomediterranean), Pinheiro da Cruz and Vila Nova de Milfontes (termomediterranean). All sampled acacia plants were located underneath a pine forest, with the exception of the latter case study, where we also sampled in a diversified habitat conditions associated with the margins of agriculture fields. According to the historical records, it was also possible to compare acacia samples from original plantations and recently invaded areas. We have used two molecular markers -inter-simple sequence repeats (ISSR) and microsatellites (SSR). ISSR analysis showed that A. longifolia populations at all sampled locations have similar levels of diversity and a relatively low differentiation ( PT = 0.135). However, samples tended to cluster according to the regional (macro-scale) collection site. Microsatellites confirmed this low differentiation pattern. Our results indicate that differences in phenology and reproductive success observed during a previous study cannot be accounted for by genetic diversity and differentiation alone. Furthermore, our results taken together with historical information of A. longifolia suggest a single original introduction and a subsequent acclimation process.

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Section: Introductionmentioning

confidence: 87%

Genetic diversity and differentiation of invasive Acacia longifolia in Portugal

2018

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“…Twenty nuclear microsatellite loci previously characterized for A. dealbata (Guillemaud et al 2015) were initially tested for amplification success and polymorphism in a subset of samples. Ten of these loci showed consistent and goodquality amplification products and were therefore used for further genotyping of all A. dealbata samples (Table S1, Online Resource).…”

Section: Genotypingmentioning

confidence: 99%

Historical range contraction, and not taxonomy, explains the contemporary genetic structure of the Australian tree Acacia dealbata Link

Hirsch

Richardson

Impson

et al. 2018

Tree Genetics & Genomes

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Irrespective of its causes, strong population genetic structure indicates a lack of gene flow. Understanding the processes that underlie such structure, and the spatial patterns it causes, is valuable for conservation efforts such as restoration. On the other hand, when a species is invasive outside its native range, such information can aid management in the non-native range. Here we explored the genetic characteristics of the Australian tree Acacia dealbata in its native range. Two subspecies of A. dealbata have previously been described based on morphology and environmental requirements, but recent phylogeographic data raised questions regarding the validity of this taxonomic subdivision. The species has been widely planted within and outside its native Australian range and is also a highly successful invasive species in many parts of the world. We employed microsatellite markers to investigate the population genetic diversity and structure among 42 A. dealbata populations from across the species' native range. We also tested whether environmental variables purportedly relevant for the putative separation of subspecies are linked with population genetic differentiation. We found no relationship between population genetic structure of A. dealbata in Australia and these environmental features. Rather, we identified two geographically distinct genetic clusters that corresponded with populations in the northeastern part of mainland Australia, and the southern mainland and Tasmanian range of the species. Our results do not support the taxonomic subdivision of the species into two distinct subspecies based on environmental features. We therefore assume that the observed morphological differences between the putative subspecies are plastic phenotypic responses. This study provides population genetic information that will be useful for the conservation of the species within Australia as well as to better understand the invasion dynamics of A. dealbata.

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Occurrence of polyploidy in populations of Acacia dealbata in south-eastern Tasmania and cytotypic variation in reproductive traits

et al. 2018

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The frequency of polyploid trees in 10 populations of the predominantly diploid species Acacia dealbata subsp. dealbata Link in south-eastern Tasmania was determined using flow cytometry. At seven of the sites, all trees were diploid. At two sites, single triploid genets were found and, at a third, two tetraploids. Microsatellite markers were used to confirm that triploid trees distributed over an area of at least 930 m2 at the major study site were all ramets of a single genet. Three diploid clones were also confirmed at this site. The 16-grain polyads from the triploid genet were significantly larger than those from diploids, but only the diploid pollen showed any viability in vitro. At three months the green pods on diploids averaged 36 mm and contained four developing seeds per pod. Pods on the triploid were only 13 mm long, with no developing seeds. In spite of maturing pods, two of the diploids did not yield full seed, whereas two other diploids averaged only 0.1 seeds per pod. Seeds were not produced on triploid trees. The low reproductive output is discussed with reference to the breeding system and the impact of clonality on effective cross-pollination.

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Development of 23 polymorphic microsatellite loci in invasive silver wattle, Acacia dealbata (Fabaceae)

Cited by 5 publications

References 13 publications

Genetic diversity and differentiation of invasive <i>Acacia longifolia</i> in Portugal

Genetic diversity and differentiation of invasive <i>Acacia longifolia</i> in Portugal

Historical range contraction, and not taxonomy, explains the contemporary genetic structure of the Australian tree Acacia dealbata Link

Occurrence of polyploidy in populations of Acacia dealbata in south-eastern Tasmania and cytotypic variation in reproductive traits

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Development of 23 polymorphic microsatellite loci in invasive silver wattle, Acacia dealbata (Fabaceae)

Cited by 5 publications

References 13 publications

Genetic diversity and differentiation of invasive &lt;i&gt;Acacia longifolia&lt;/i&gt; in Portugal

Genetic diversity and differentiation of invasive &lt;i&gt;Acacia longifolia&lt;/i&gt; in Portugal

Historical range contraction, and not taxonomy, explains the contemporary genetic structure of the Australian tree Acacia dealbata Link

Occurrence of polyploidy in populations of Acacia dealbata in south-eastern Tasmania and cytotypic variation in reproductive traits

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Genetic diversity and differentiation of invasive <i>Acacia longifolia</i> in Portugal

Genetic diversity and differentiation of invasive <i>Acacia longifolia</i> in Portugal