Development of 18 microsatellite markers for Salvia pratensis

Abstract

Salvia pratensis L. (Lamiaceae) is a perennial herb distributed throughout Europe from the Volga River in the east, to northern Spain in the west, to the southern European peninsulas (Balkan and Apennine) in the south, and to the Netherlands, Poland, and Germany in the north (Kaplan et al., 2018). A few locations are also recorded in the British Isles, where the species is protected (Kaplan et al., 2018). The species is diploid with 2n = 2x = 18 chromosomes (e.g., Van Loon, 1980). It is a typical representative of European temperate dry grassland vegetation. Grasslands represent one of the most species-rich but threatened ecosystems in temperate Europe (Habel et al., 2013). Understanding the phylogeographic history of such species is crucial for determining the postglacial history of these endangered ecosystems and is expected to facilitate the development of efficient conservation strategies.A total of 29 microsatellite markers have already been developed for S. officinalis L. (Molecular Ecology Resources Primer Development Consortium et al., 2010;Radosavljević et al., 2011Radosavljević et al., , 2012, a species representing a sister group to S. pratensis (Will and Claßen-Bockhoff, 2017). Twenty of these loci were tested for cross-amplification to S. pratensis, but successful cross-amplification has been reported for only five (Radosavljević et al., 2011). For a state-of-the-art computational approach, this low number of microsatellite markers is inadequate for a comprehensive phylogeographical or population genetic study. Therefore, we set out to develop a set of markers specifically for S. pratensis. This marker set was tested for cross-amplification in other species of Salvia L.

Development of a Large Gene-Associated SSR Marker Set and in-Depth Genetic Characterization in Scarlet Sage

“…Nine SSR markers were developed from Salvia officinalis L., with the Ho, He, PIC ranged from 0.46 to 0.83, 0.73 to 0.93, and 0.70 to 0.92, respectively (Radosavljević et al, 2012). Similar level of genetic diversity was also seen for other Salvia species (ÝNce and Karaca, 2015;Krak et al, 2020). The SSR markers were identified to be highly polymorphic.…”

Genome-Wide Development of Polymorphic Microsatellite Markers and Genetic Diversity Analysis for the Halophyte Suaeda aralocaspica (Amaranthaceae)

“…Genomic DNA from S. aralocaspica plant material was extracted using the DNAsecure Plant Kit (Tiangen Biotechnology, Beijing, China), following the manufacturer's guidelines. From all genomic SSRs, 100 candidate primer pairs were randomly selected based on the minimum lengths of 14, 18, and 20 bp for di-, tri-, and tetranucleotide repeats [42,43], respectively. The forward primers' 5 ends were labeled with FAM blue, a fluorescent dye (Shanghai General Biotechnology, Shanghai, China), for easy scoring in genotyping.…”