Development of 18 microsatellite loci for the freshwater snail Bellamya aeruginosa (Mollusca, Gastropoda)

Qh, Gu; Bx, Xiong; Zhu, Yuhao; Wang, Q; Ps, Shi

doi:10.4238/2012.may.18.4

Cited by 4 publications

(2 citation statements)

References 13 publications

(13 reference statements)

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“…Many of the species of this genus have been named endangered species, including B. limnophila, B. lithophaga, B. manhungensis, B. papillapicala, and B. smith (Gu et al, 2012a).…”

Section: Introductionmentioning

confidence: 99%

Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species

Qh¹,

Qq²,

Xj³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Bellamya is a widely distributed freshwater snail genus in China; however, its genetic diversity is completely unknown. Sixtyfive novel microsatellite loci were isolated and characterized from a microsatellite-enriched library of Bellamya aeruginosa genomic DNA. Most of the 65 loci were successfully amplified. We found high polymorphic information content values for these loci, ranging from 0.235 to 0.892. There were 3 to 12 alleles per locus, and the H E and H O varied from 0.425 to 0.953 and 0.026 to 1.000, respectively. Fifteen loci deviated significantly from Hardy-Weinberg equilibrium after Bonferroni's correction. All 65 SSR markers were tested in an additional five Bellamya species, and 96.9% of the 325 locus/taxon combinations tested resulted in cross-species amplification. Seven polymorphic microsatellite markers were randomly selected for comparison among nine populations of three species. All populations had moderate to high genetic diversity. In genetic distance-based cluster analysis, the populations of B. aeruginosa and B. dispiralis formed species-based clusters, whereas populations of B. angularia did 15202 Q.H. Gu et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15201-15212 (2015) not. The three examined Bellamya species could be differentiated using SSR markers. These microsatellite loci should be useful for genetic diversity analysis, analysis of phylogenetic relationship, and species delimitation of Bellamya.

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“…Many of the species of this genus have been named endangered species, including B. limnophila, B. lithophaga, B. manhungensis, B. papillapicala, and B. smith (Gu et al, 2012a).…”

Section: Introductionmentioning

confidence: 99%

Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species

Qh¹,

Qq²,

Xj³

et al. 2015

Genet. Mol. Res.

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“…In addition, SSRs have been developed and studied in members of the lymnaeid genera, namely; Lymnaea truncatula (Trouve et al, 2000), Lymnaea stagnails (Knott et al, 2003), Lymnaea auricularia (de Boer et al, 2004) and Radix balthica (Salinger and Pfenninger, 2009). In freshwater snails, SSRs were successfully crossamplified between species/within genus (Trouve et al, 2000;Gow et al, 2001;Wilmer et al, 2005;Dubois et al, 2008;Liu and Hershler, 2008;Dupuy et al, 2009;Salinger and Pfenninger, 2009;Gu et al, 2012aGu et al, , 2012bGu et al, , 2015 and between genera/within family (Wilmer et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in Radix species from the middle and south of Iraq based on simple sequence repeats

Al-Waaly¹,

Triwitayakorn²,

Srisawad³

et al. 2018

Afr. J. Biotechnol.

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Ten newly developed microsatellite loci were isolated from an AG 20 and CAG 20 enriched genomic DNA library of Radix sp. from Iraq. The simple sequence repeats (SSR) comprised 76.0% di-, 20.0% tri-and 4.0% tetra-nucleotide repeats. The number of alleles for all 10 loci ranged from 4 to 18, with a mean of 11.3 alleles per locus. The observed (Ho) and expected heterozygosity (He) values varied from 0.286 to 0.927 (mean 0.640) and from 0.263 to 0.939 (mean 0.753), respectively. The polymorphic information content (PIC) values ranged from 0.336 to 0.923 (mean 0.749). Five loci showed significant deviation from Hardy-Weinberg equilibrium (HWE) and no significant linkage disequilibrium (LD) was observed. Cross transferability was successfully tested on species from the genus Melanopsis. These polymorphic SSRs will be useful for assessment of the genetic diversity and population genetics of a Radix sp. as well as other related species in Iraq.

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Human‐geographic effects on variations in the population genetics of Sinotaia quadrata (Gastropoda: Viviparidae) that historically migrated from continental East Asia to Japan

Saito

Hirano

et al. 2020

Ecology and Evolution

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Background Anthropogenic factors potentially affect observed biogeographical patterns in population genetics, but the effects of ancient human activities on the original patterns created by natural processes are unknown. Sinotaia quadrata , a widely distributed freshwater snail species in East Asia, was used to investigate this issue. It is unclear whether S. quadrata in Japan was introduced from China and how different human uses and varying geographic patterns affect the contemporary population genetics between the two regions. Thus, we investigated the demography of S. quadrata and detected its genetic structure in Japan and continental East Asia. Results Sinotaia quadrata populations first naturally migrated from continental East Asia to Japan, which is associated with the ancient period in Japanese geohistory (about 70,000 years ago). They were then artificially introduced in association with agriculture expansion by human movements in two recent periods (about 8,000 and 1,200 years ago). Populations in different parts of Japan have their own sources. Natural migration in the ancient period and artificial introduction in the recent period suggest that the population distribution is affected by both the geohistory of East Asia and the history of human expansion. In the background of the historical migration and introduction, contemporary populations in the two regions show different genetic patterns. Population divergence levels were significantly correlated with geographical patterns in Japan and significantly correlated with human interventions variables in continental East Asia, suggesting that long‐term geographical isolation is likely the major factor that shaped the contemporary population genetics in Japan, while modern human uses are likely the major factor in continental East Asia. Conclusions Our preliminary results show a complex demography and unusual genetic patterns in the contemporary populations for a common freshwater snail and are of significance to determine the historical formation and contemporary patterns of biogeography in Japan and continental East Asia.

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Development of 18 microsatellite loci for the freshwater snail Bellamya aeruginosa (Mollusca, Gastropoda)

Cited by 4 publications

References 13 publications

Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species

Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species

Genetic diversity in Radix species from the middle and south of Iraq based on simple sequence repeats

Human‐geographic effects on variations in the population genetics of Sinotaia quadrata (Gastropoda: Viviparidae) that historically migrated from continental East Asia to Japan

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