Development of 16S rRNA-Based Probes for the
 Coriobacterium
 Group and the
 Atopobium
 Cluster and Their Application for Enumeration of
 Coriobacteriaceae
 in Human Feces from Volunteers of Different Age Groups

Harmsen, Hermie J. M.; Wildeboer-Veloo, A. C. M.; Grijpstra, Jan; Knol, Jan; Degener, John E.; Welling, Gjalt W.

doi:10.1128/aem.66.10.4523-4527.2000

Cited by 283 publications

(176 citation statements)

References 29 publications

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“…Dose period 3 (days [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44]. Volunteers consumed 100 g of their treatment chocolate, and kept daily diaries during the period.…”

Section: Experimental Designmentioning

confidence: 99%

A human volunteer study to assess the impact of confectionery sweeteners on the gut microbiota composition

2010

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Sweeteners are being sourced to lower the energetic value of confectionery including chocolates. Some, especially non-digestible carbohydrates, may possess other benefits for human health upon their fermentation by the colonic microbiota. The present study assessed non-digestible carbohydrate sweeteners, selected for use in low-energy chocolates, for their ability to beneficially modulate faecal bacterial profiles in human volunteers. Forty volunteers consumed a test chocolate (low-energy or experimental chocolate) containing 22·8 g of maltitol (MTL), MTL and polydextrose (PDX), or MTL and resistant starch for fourteen consecutive days. The dose of the test chocolates was doubled every 2 weeks over a 6-week period. Numbers of faecal bifidobacteria significantly increased with all the three test treatments. Chocolate containing the PDX blend also significantly increased faecal lactobacilli (P¼ 0·00 001) after the 6 weeks. The PDX blend also showed significant increases in faecal propionate and butyrate (P¼ 0·002 and 0·006, respectively). All the test chocolates were well tolerated with no significant change in bowel habit or intestinal symptoms even at a daily dose of 45·6 g of non-digestible carbohydrate sweetener. This is of importance not only for giving manufacturers a sugar replacement that can reduce energetic content, but also for providing a well-tolerated means of delivering high levels of non-digestible carbohydrates into the colon, bringing about improvements in the biomarkers of gut health.

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“…Dose period 3 (days [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44]. Volunteers consumed 100 g of their treatment chocolate, and kept daily diaries during the period.…”

Section: Experimental Designmentioning

confidence: 99%

A human volunteer study to assess the impact of confectionery sweeteners on the gut microbiota composition

2010

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“…Probes ATO291 (probeBase accession no. pB-00943; name S-*-Ato-0291-a-A-17; 59-GGTCGGTCTCTCAACCC-39; Harmsen et al, 2000) and EUB338mix [(pB-0159; S-D-Bact-0338-a-A-18; 59-GCTGC-CTCCCGTAGGAGT-39), (pB-0160; S-*-BactP-0338-a-A-18; 59-G-CAGCCACCCGTAGGTGT-39), (pB-0161; S-*-BactV-0338-a-A-18; 59-GCTGCCACCCGTAGGTGT-39); Daims et al, 1999] were used to enumerate 'Atopobium cluster' and total bacteria, respectively. Slides were examined under a Nikon E400 Eclipse epifluorescence microscope.…”

Section: Methodsmentioning

confidence: 99%

“…were detected by the probe (Harmsen et al, 2000). The bacteria targeted by this group were referred to as the 'Atopobium cluster'.…”

Section: Fish Analysis Of Faecal 'Atopobium Cluster' Populationmentioning

confidence: 99%

“…Much is known about the diversity of the Firmicutes, Bacteroidetes, Proteobacteria and the genus Bifidobacterium (Actinobacteria) within the GI tract. Even though more reliable cell-based quantification methods such as FISH indicate they are numerically more predominant than the bifidobacteria in human faeces, representing approximately 8 % of the total bacteria, little is known about the diversity and metabolic abilities of Actinobacteria belonging to the class Coriobacteriia (Harmsen et al, 2000;Rigottier-Gois et al, 2003;Lay et al, 2005;Child et al, 2006). However, Collinsella aerofaciens is part of the core gut microbiome of healthy and obese adults (Turnbaugh et al, 2009;Qin et al, 2010), and Adlercreutzia and Slackia species have been associated with equal production in the human GI tract (Maruo et al, 2008;Jin et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…representation as determined by quantitative PCR (qPCR) and FISH (Harmsen et al, 2000(Harmsen et al, , 2002Matsuki et al, 2004). It is well known that Actinobacteria, specifically bifidobacteria, are under-represented or remain undetected in PCRbased studies, with factors such as selection of DNA extraction method, PCR primers and cycling conditions affecting their representation in clone libraries (Wilson & Blitchington, 1996;Suau et al, 1999;Koenig et al, 2011;Maukonen et al, 2012;Sim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Dynamics and diversity of the ‘Atopobium cluster' in the human faecal microbiota, and phenotypic characterization of ‘Atopobium cluster' isolates

2015

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This study monitored the dynamics and diversity of the human faecal 'Atopobium cluster' over a 3-month period using a polyphasic approach. Fresh faecal samples were collected fortnightly from 13 healthy donors (six males and seven females) aged between 26 and 61 years. FISH was used to enumerate total (EUB338mix) and 'Atopobium cluster' (ATO291) bacteria, with counts ranging between 1.12¾10 11 and 9.95¾10 11 , and 1.03¾10 9 and 1.16¾10 11 cells (g dry weight faeces) "1 , respectively. The 'Atopobium cluster' population represented 0.2-22 % of the total bacteria, with proportions donor-dependent. Denaturing gradient gel electrophoresis (DGGE) using 'Atopobium cluster'-specific primers demonstrated faecal populations of these bacteria were relatively stable, with bands identified as Collinsella aerofaciens, Collinsella intestinalis/ Collinsella stercoris, Collinsella tanakaei, Coriobacteriaceae sp. PEAV3-3, Eggerthella lenta, Gordonibacter pamelaeae, Olsenella profusa, Olsenella uli and Paraeggerthella hongkongensis in the DGGE profiles of individuals. Colony PCR was used to identify 'Atopobium cluster' bacteria isolated from faeces (n5224 isolates). 16S rRNA gene sequence analysis of isolates demonstrated Collinsella aerofaciens represented the predominant (88 % of isolates) member of the 'Atopobium cluster' found in human faeces, being found in nine individuals. Eggerthella lenta was identified in three individuals (3.6 % of isolates). Isolates of Collinsella tanakaei, an 'Enorma' sp. and representatives of novel species belonging to the 'Atopobium cluster' were also identified in the study. Phenotypic characterization of the isolates demonstrated their highly saccharolytic nature and heterogeneous phenotypic profiles, and 97 % of the isolates displayed lipase activity.

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Gram-Positive Anaerobic Cocci

Wren

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Development of 16S rRNA-Based Probes for the Coriobacterium Group and the Atopobium Cluster and Their Application for Enumeration of Coriobacteriaceae in Human Feces from Volunteers of Different Age Groups

Cited by 283 publications

References 29 publications

A human volunteer study to assess the impact of confectionery sweeteners on the gut microbiota composition

A human volunteer study to assess the impact of confectionery sweeteners on the gut microbiota composition

Dynamics and diversity of the ‘Atopobium cluster' in the human faecal microbiota, and phenotypic characterization of ‘Atopobium cluster' isolates

Gram-Positive Anaerobic Cocci

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