Development of 12 tetranucleotide microsatellite markers for the tetraploidy fish Percocypris pingi (Tchang)

Deng, Yong; Yang, Kun; Gan, Weixiong; Zeng, Rukui; Yan, Pengfei; Song, Zhaobin

doi:10.1007/s12686-014-0302-9

Cited by 1 publication

(4 citation statements)

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“…Twelve tetranucleotide microsatellite markers were used for analyses: Seven (PM49, PM32, PM25, PM23, PM20, PM19 and PM15) from Deng et al [13] and another five (PM10, PM17, PM16, PM08, PM40) from Deng [32] (see S1 Table for details on the loci). Each forward primer of the loci was labeled by fluorescent dyes–FAM, TAMRA or HEX–for fragment visualization.…”

Section: Methodsmentioning

confidence: 99%

“…Each forward primer of the loci was labeled by fluorescent dyes–FAM, TAMRA or HEX–for fragment visualization. A polymerase chain reaction (PCR) was performed using a standard PCR protocol described in Deng et al [13]. …”

Section: Methodsmentioning

confidence: 99%

“…The microsatellite genetic diversity estimation, the tests for Hardy–Weinberg equilibrium (HWE), and linkage disequilibrium among loci followed Deng et al [13]. We evaluated the mean number of alleles per locus and per population ( A ), mean allelic richness across all loci per locus and per population ( A i ), the polymorphic information content (PIC), the observed heterozygosity ( H o ), and expected heterozygosity under random chromosome segregation ( H e ( c e )) and under some level of chromatid segregation ( H e ( c d )).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies of P . pingi focused more on morphology, anatomy, nutriology, and artificial propagation with a few studies involved with molecular genetic issues [11,12,13]. Only a single study conducted by Yue et al [14] investigated the genetic diversity of one P .…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Structure Analysis of Percocypris pingi (Cypriniformes: Cyprinidae): Implications for Conservation and Hatchery Release in the Yalong River

Deng

Yang

et al. 2016

PLoS ONE

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Percocypris pingi is a near threatened cyprinid species, which has suffered a dramatic decline due to anthropogenic factors. As one response to this decline, hatchery release for P. pingi has been conducted in the lower reaches of the Yalong River since 2012. To understand the conservation status of this species and the potential impact of the release of hatchery-reared fish, we studied the genetic diversity and population structure of wild and hatchery populations of P. pingi. Two hatchery populations (Jinping [JPH] and Ya’an [YAH]) and two wild populations (Muli [MLW] and Woluo [WLW]) of P. pingi were analyzed based on microsatellite markers and the mitochondrial DNA control region. The results showed that P. pingi possesses moderate levels of genetic diversity, with observed heterozygosities ranging from 0.657 to 0.770 and nucleotide diversities ranging from 0.00212 to 0.00491. Our results also suggested WLW harbors considerable proportions of genetic diversity in this species and serves as a refuge for P. pingi during anthropogenic disturbance, thus playing an important role for the conservation of P. pingi populations. Microsatellite and mitochondrial markers both indicated close genetic relationships between YAH and MLW, JPH and WLW, respectively. The results to some extent reflected the geographical provenances for original broodstocks of the two hatchery populations, which provide some practical guidance for hatchery release of P. pingi. The existence of remarkable genetic divergence distributed along limited geographical range (approximately 10 kilometers) suggests the two wild populations should be regarded at least as two distinct evolutionary significant units (ESUs) and management units (MUs). Considering reduced intra-population genetic variation in hatchery population for release and significant genetic compositions of the two hatchery populations, some appropriate breeding strategies were proposed to benefit conservation of P. pingi.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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