Development of 101 novel SSR markers and construction of an SSR-based genetic linkage map in cucumber (<i>Cucumis sativus</i> L.)

A linkage map of cultivated cucumber (Cucumis sativus L.) with 248 microsatellite marker loci and seven genes for horticulturally important traits

“…A moderately saturated or high-density linkage map and closely linked molecular markers for target genes are two requirements for efficient MAS. In cucumber, however, due to its narrow genetic base and thus low polymorphisms (Kennard et al 1994), although nearly 30 linkage maps have been constructed, the majority of molecular markers placed on these lowresolution genetic maps are RAPDs (randomly amplified polymorphic DNAs) or AFLPs (amplified fragment length polymorphism) (e.g., Kennard et al 1994;Serquen et al 1997;Park et al 2000;Fazio et al 2003;Robbins et al 2008;Fukino et al 2008;Yuan et al 2008) that are not breeder friendly. In additional, only a few horticulturally important genes or QTL (quantitative trait loci) of cucumber have been mapped with molecular markers (e.g., Kennard and Havey 1995;Sakata et al 2006;Lui et al 2008;Yuan et al 2008).…”

Development of Simple Sequence Repeat Markers for Identification of Japanese Gentian Cultivars

“…The other 116 unique SSR sequences could not be used, because the sequences proximal to the SSR motif were too short to design appropriate primers. The frequency with which primers could be designed in Japanese gentian (45.0%) was lower than in other species, such as eggplant (75.0%, Nunome et al, 2009) and cucumber (96.3%, Fukino et al, 2008). One of the reasons was because we attempted to design primers with a uniform annealing temperature of 60°C to ensure reproducible and reliable amplification.…”

“…A total of 475 clones were subjected to sequence analysis, of which 211 clones (44.4%) had unique sequences containing SSR motifs (Table 2). In comparison, the SSR-enriched libraries constructed using the same method contained 8.7-37.6%, 30.2%, and 14.1% unique SSR loci in bunching onion (Tsukazaki et al, 2007), eggplant (Nunome et al, 2009), and cucumber (Fukino et al, 2008), respectively. In G. crassicaulis, Li et al (2007) reported that 34.2% contained SSR sequences in the AC/ TG-enriched library constructed by combining with the biotin capture method.…”

“…SSRs are tandemly repeated sequence motifs 1-6 bp in length and are widely present in the eukaryotic genome. Therefore, SSR markers have been applied to discriminate cultivars in many crops, such as soybean (Hossain et al, 2000), fruit trees including apple (Goulão and Oliveira, 2001), olive (Díaz et al, 2006), peach (Dirlewanger et al, 2002;Yamamoto et al, 2003), sweet cherry (Dirlewanger et al, 2002), pear (Kimura et al, 2002), Japanese apricot (Hayashi et al, 2008), and almond (Shiran et al, 2007), in vegetable crops including melon (Chiba et al, 2003), strawberry (Shimomura and Hirashima, 2006), eggplant (Nunome et al, 2009), Brassica rapa (Suwabe et al, 2002, and cucumber (Fukino et al, 2008), and floricultural plants including pelargonium (Becher et al, 2000) and carnation (Smulders et al, 2003). In Gentiana, ten SSR markers were developed for the Chinese medicinal plant G. crassicaulis (Li et al, 2007); however, since G. crassicaulis belongs to a different taxonomic section from the Japanese cultivated gentians G. triflora and G. scabra (Ho and Liu, 2001), these SSR markers could not be successfully applied to Japanese gentians.…”

Chromosomal location and mode of inheritance of a gene conferring resistance to fusarium wilt inCucumis anguriaL.

“…Seventy-two simple sequence repeat (SSR) markers developed in C. sativus L. (Fukino et al, 2008;Ren et al, 2009;Watcharawongpaiboon and Chunwongse, 2008) and in melon [Chiba et al, 2003; Cucurbit Genomics Database (http://www.icugi.org/); Danin-Poleg et al, 2001;Fukino et al, 2007;Gonzalo et al, 2005;Ritschel et al, 2004], and confirmed to be polymorphic between PI 320052 and PI 364475, were used for the construction of a genetic linkage map.…”