Development Method for Determination of Aspirin as Sodium Salicylate by UV-VIS Spectroscopy

Albakaa, Ali Rasool Mahmood; Ahmed, Mohammed Abdullah; mohammed, Benas thamer; Jabbar, Zainab A.

doi:10.1088/1757-899x/571/1/012104

Cited by 6 publications

(1 citation statement)

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“…Salicylic acid forms a violet complex with ferric nitrate, which exhibits a maximum absorbance at 535 nm. The intensity of the violet color is a function of the concentration of salicylate in the solution . Therefore, the absorbance of the salicylate solution was measured at 535 nm following the reaction between the salicylate with the ferric nitrate.…”

Section: Methodsmentioning

confidence: 99%

Assembly of Microbial Communities Engaging in Metabolic Division of Labor in a Diffusion-Limited Environment Is Governed by Metabolic Flux

et al. 2023

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Metabolic division of labor (MDOL) represents one of the most commonly occurring interactions within natural microbial communities. Specifically, in a variety of MDOL systems engaged in hydrocarbon degradation, a sequential degradation is performed by several members with final products that are necessary for the growth of each member. In these MDOL systems, each strain catalyzes one or more specific reactions of a multistep metabolic pathway, whose end products are then allocated among the participants. While the benefit allocation is independent of metabolic flux in well-mixed environments, it remains unclear how the benefits are allocated when diffusion is limited. Here, we investigated how MDOL communities assemble in a diffusion-limited environment, by combining mathematical modeling with experimental inquiry using a synthetic consortium engaged in MDOL. Our model analysis in a diffusion-limited environment showed that, when the growth of all populations in the community relies on the final product that can only be produced by the last population, a diffusion gradient of the final products may create a bias favoring the member producing the final products, resulting in a higher relative abundance of the final product producer. Moreover, such asymmetric allocation of the final products is enhanced by both the lower diffusion rate and the higher metabolic flux (i.e., the higher yields of the final products) in the MDOL. Our results show that in a diffusively confined environment, metabolic flux constitutes a determining factor in the assembly of the MDOL community. Together, our findings are critical for a better understanding of how resource-sharing microbial communities are established and should assist in designing such communities for improved biomanufacturing and bioremediation.

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Section: Methodsmentioning

confidence: 99%