Development, Diversity and Dynamics of Plant Architecture in Utricularia subgenus Polypompholyx – Towards Understanding Evolutionary Processes in the Lentibulariaceae

Reut, Markus S.; Płachno, Bartosz J.

doi:10.1007/s12229-022-09283-5

Cited by 5 publications

(2 citation statements)

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“…Recently, Carmesin et al [ 18 ] measured the hydraulic resistance of traps but did not observe water currents inside the traps of G. hispidula . Finally, species of the genus Utricularia (bladderworts) are also rootless as Genlisea , but with the more complex morphology of vegetive organs [ 19 , 20 ]. They form bladders ( Figure 1 ) with a high-speed suction mechanism [ 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Microdomains in the External Glands of Utricularia dichotoma Traps

Płachno,

Kapusta,

Stolarczyk

et al. 2024

IJMS

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The genus Utricularia (bladderworts) species are carnivorous plants that prey on invertebrates using traps with a high-speed suction mechanism. The outer trap surface is lined by dome-shaped glands responsible for secreting water in active traps. In terminal cells of these glands, the outer wall is differentiated into several layers, and even cell wall ingrowths are covered by new cell wall layers. Due to changes in the cell wall, these glands are excellent models for studying the specialization of cell walls (microdomains). The main aim of this study was to check if different cell wall layers have a different composition. Antibodies against arabinogalactan proteins (AGPs) were used, including JIM8, JIM13, JIM14, MAC207, and JIM4. The localization of the examined compounds was determined using immunohistochemistry techniques and immunogold labeling. Differences in composition were found between the primary cell wall and the cell secondary wall in terminal gland cells. The outermost layer of the cell wall of the terminal cell, which was cuticularized, was devoid of AGPs (JIM8, JIM14). In contrast, the secondary cell wall in terminal cells was rich in AGPs. AGPs localized with the JIM13, JIM8, and JIM14 epitopes occurred in wall ingrowths of pedestal cells. Our research supports the hypothesis of water secretion by the external glands.

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Section: Introductionmentioning

confidence: 99%

Cell Wall Microdomains in the External Glands of Utricularia dichotoma Traps

Płachno,

Kapusta,

Stolarczyk

et al. 2024

IJMS

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“…All of the Utricularia species are rootless herbs. Their vegetative organs go beyond the typical organ classification, i.e., either ‘root’, ‘stem’, or ‘leaf’, and they have intermixed morphological traits and developmental programs [ 5 , 6 , 7 , 8 ]. These are related to the heterotopic transfer of the function of the genes to other organs, as was shown in the root genes [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Localization of Cell Wall Components in the Quadrifids of Whole-Mount Immunolabeled Utricularia dichotoma Traps

Płachno,

Kapusta

2023

IJMS

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Utricularia (bladderworts) are carnivorous plants. They produce small hollow vesicles, which function as suction traps that work underwater and capture fine organisms. Inside the traps, there are numerous glandular trichomes (quadrifids), which take part in the secretion of digestive enzymes, the resorption of released nutrients, and likely the pumping out of water. Due to the extreme specialization of quadrifids, they are an interesting model for studying the cell walls. This aim of the study was to fill in the gap in the literature concerning the immunocytochemistry of quadrifids in the major cell wall polysaccharides and glycoproteins. To do this, the localization of the cell wall components in the quadrifids was performed using whole-mount immunolabeled Utricularia traps. It was observed that only parts (arms) of the terminal cells had enough discontinuous cuticle to be permeable to antibodies. There were different patterns of the cell wall components in the arms of the terminal cells of the quadrifids. The cell walls of the arms were especially rich in low-methyl-esterified homogalacturonan. Moreover, various arabinogalactan proteins also occurred. Cell walls in glandular cells of quadrifids were rich in low-methyl-esterified homogalacturonan; in contrast, in the aquatic carnivorous plant Aldrovanda vesiculosa, cell walls in the glandular cells of digestive glands were poor in low-methyl-esterified homogalacturonan. Arabinogalactan proteins were found in the cell walls of trap gland cells in all studied carnivorous plants: Utricularia, and members of Droseraceae and Drosophyllaceae.

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