Development‐dependent localization of nuclear antigens in growing mouse oocytes

Borsuk, Ewa; Vautier, Dominique; Szöllösi, Maria S.; Besombes, Didier; Debey, Pascale

doi:10.1002/(sici)1098-2795(199603)43:3<376::aid-mrd12>3.0.co;2-#

Cited by 13 publications

(1 citation statement)

References 39 publications

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“…Moreover, we have found that inhibition of RNA synthesis in oocytes obtained from 12-days old females, resulted in the condensation of chromatin and reorganization of insoluble form of Mcm2 and Mcm7. Formation of large aggregates, occurring concomitantly with transcriptional silencing and condensation of chromatin (either physiological in fully-grown SN oocytes, or induced by α-amanitin treatment of oocytes obtained from 12-days old mouse females), was previously observed in the case of splicing factor SC-35 (Borsuk et al, 1996). We can not exclude that such rearrangement of Mcm-positive structures occurs via a passive movement with condensing chromatin, having no relation to silencing of the transcription.…”

Section: Mcm2 6 and 7 In Mouse Oocytes And One-cell Embryo 289mentioning

confidence: 99%

Accumulation and dynamics of proteins of the MCM family during mouse oogenesis and the first embryonic cell cycle

Swiech¹,

Kisiel²,

Czołowska³

et al. 2007

Int. J. Dev. Biol.

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Section: Mcm2 6 and 7 In Mouse Oocytes And One-cell Embryo 289mentioning

confidence: 99%

Accumulation and dynamics of proteins of the MCM family during mouse oogenesis and the first embryonic cell cycle

Swiech¹,

Kisiel²,

Czołowska³

et al. 2007

Int. J. Dev. Biol.

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Chromatin configuration and transcriptional control in human and mouse oocytes

Miyara¹,

Migné²,

Dumont-Hassan³

et al. 2003

Molecular Reproduction Devel

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In vitro maturation of human oocytes at the germinal vesicle (GV) stage could offer an alternative in several cases of female infertility. It however rests on a better knowledge of the quality of human oocyte. Using fluorescence imaging of DNA and of the transcription sites, combined with electron microscopy, we show that human oocytes follow size-dependent changes in chromatin configuration, transcription sites distribution and nuclear ultrastructure that follow those observed in mouse GV oocytes. We thus analyzed in mouse GV oocytes the phosphorylation dependence of the transcriptional activity. We show by Western blot that, while active GV oocytes have approximately the same proportion of hypo- and hyperphosphorylated forms of the RNA polymerase II (RNAP II), the hyperphosphorylated form is almost absent from inactive oocytes. We also show that (1) RNAP II-dependent transcription is much less sensitive to various kinase inhibitors in mouse oocytes than in somatic cells or mouse one-cell embryos, although the phosphorylation equilibrium of RNAP II was largely shifted towards the hypo-phosphorylated form upon treatment with these inhibitors (2) RNAP I is completely insensitive to kinase inhibitors in GV oocytes.

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Nuclear localization of NORs and centromeres in mouse oocytes during folliculogenesis

Longo

Garagna

Merico

et al. 2003

Molecular Reproduction Devel

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Mouse oocytes at the germinal vesicle stage are characterized by one of two nuclear morphologies: surrounded nucleolus (SN), in which the nucleolus is surrounded by a rim of Hoechst positive chromatin and not surrounded nucleolus (NSN), in which this rim is essentially absent. This morphological difference has a biological relevance as NSN oocytes are transcriptionally active, yet incapable of development beyond the two-cell stage. Whereas SN oocytes, which are transcriptionally inactive, are capable of development to the blastocyst stage. To further our understanding of the nuclear organization of the mouse oocyte during folliculogenesis, we have conducted a series of investigations employing silver methods that stain nucleolus organizer region (NOR), centromeres, and heterochromatin, as well as, specific antibodies for centromeres. Results obtained by a variety of microscopic methods (light, electron, immunochemical, and confocal) demonstrate: (1) a changing pattern of NOR staining during folliculogenesis that is specific to follicular type, and (2) significant differences in the organization of NORs and centromeres of isolated, antral NSN, and SN oocytes. These observations suggest possible means by which, chromosomes of mature, germinal vesicle oocytes are organized with respect to the nucleolus.

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Development‐dependent localization of nuclear antigens in growing mouse oocytes

Cited by 13 publications

References 39 publications

Accumulation and dynamics of proteins of the MCM family during mouse oogenesis and the first embryonic cell cycle

Accumulation and dynamics of proteins of the MCM family during mouse oogenesis and the first embryonic cell cycle

Chromatin configuration and transcriptional control in human and mouse oocytes

Nuclear localization of NORs and centromeres in mouse oocytes during folliculogenesis

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