Development, Characterization, and Use of a Fetal Skin Cell Bank for Tissue Engineering in Wound Healing

Roessingh, Anthony de Buys; Hohlfeld, Judith; Scaletta, Corinne; Hirt‐Burri, Nathalie; Gerber, Stefan; Hohlfeld, P.; Gebbers, Jan‐Olaf; Applegate, Lee Ann

doi:10.3727/000000006783981459

Cited by 72 publications

(94 citation statements)

References 13 publications

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“…When compared with fetal skin fibroblasts used in clinics [45,47] and bone-derived fetal cells (Fig. 2), it appears that primary fetal cultures are tissue specific.…”

Section: Cell Growth and Expression Of Muscle Markers In Fetal Musclementioning

confidence: 98%

“…All the muscle samples, measuring 1 cm 3 , were obtained from thigh muscle for each patient. Primary cultures of fetal skin cells and fetal bone cells were developed as described previously [45,47,56].…”

Section: Muscle Biopsies and Cell Culturementioning

confidence: 99%

“…They can be isolated from fetal tissues, which follow the embryonic stage of 9 weeks of development. Fetal cells are mature, fully differentiated cells and capable of proliferating rapidly [45,46] and have a higher resistance to oxydatif stress than adult cells [47]. Moreover, fetal tissue is not easily rejected by the recipient due to the low levels of histocompatibility antigens [43,48,49].…”

Section: Introductionmentioning

confidence: 99%

“…Applications of fetal cell therapy are also of interest in the orthopedic area, where human fetal bone cells were shown to have high proliferation and differentiation ability and could be used as a source of cells for bone tissue-engineering [53,54]. Recently, biological bandages composed of fetal skin fibroblasts improve skin regeneration in second-and thirddegree burned children [45,47]. A highly consistent fetal skin cell bank was created with which it was possible to produce more than 270 million skin constructs for therapeutic use from one organ donation [47,55].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, biological bandages composed of fetal skin fibroblasts improve skin regeneration in second-and thirddegree burned children [45,47]. A highly consistent fetal skin cell bank was created with which it was possible to produce more than 270 million skin constructs for therapeutic use from one organ donation [47,55].…”

Section: Introductionmentioning

confidence: 99%

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Human muscular fetal cells: a potential cell source for muscular therapies

Hirt‐Burri¹,

Roessingh²,

Scaletta

et al. 2007

Pediatr Surg Int

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Myoblast transfer therapy has been extensively studied for a wide range of clinical applications, such as tissue engineering for muscular loss, cardiac surgery or Duchenne Muscular Dystrophy treatment. However, this approach has been hindered by numerous limitations, including early myoblast death after injection and specific immune response after transplantation with allogenic cells. Different cell sources have been analyzed to overcome some of these limitations. The object of our study was to investigate the growth potential, characterization and integration in vivo of human primary fetal skeletal muscle cells. These data together show the potential for the creation of a cell bank to be used as a cell source for muscle cell therapy and tissue engineering. For this purpose, we developed primary muscular cell cultures from biopsies of human male thigh muscle from a 16-week-old fetus and from donors of 13 and 30 years old. We show that fetal myogenic cells can be successfully isolated and expanded in vitro from human fetal muscle biopsies, and that fetal cells have higher growth capacities when compared to young and adult cells. We confirm lineage specificity by comparing fetal muscle cells to fetal skin and bone cells in vitro by immunohistochemistry with desmin and 5.1H11 antibodies. For the feasibility of the cell bank, we ensured that fetal muscle cells retained intrinsic characteristics after 5 years cryopreservation. Finally, human fetal muscle cells marked with PKH26 were injected in normal C57BL/6 mice and were found to be present up to 4 days. In conclusion we estimate that a human fetal skeletal muscle cell bank can be created for potential muscle cell therapy and tissue engineering.

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“…When compared with fetal skin fibroblasts used in clinics [45,47] and bone-derived fetal cells (Fig. 2), it appears that primary fetal cultures are tissue specific.…”

Section: Cell Growth and Expression Of Muscle Markers In Fetal Musclementioning

confidence: 98%

Section: Muscle Biopsies and Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Human muscular fetal cells: a potential cell source for muscular therapies

Hirt‐Burri¹,

Roessingh²,

Scaletta

et al. 2007

Pediatr Surg Int

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Unbiased and quantitative proteomics reveals highly increased angiogenesis induction by the secretome of mesenchymal stromal cells isolated from fetal rather than adult skin

Gaetani

Chinnici

Carreca

et al. 2017

J Tissue Eng Regen Med

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Scarless wound healing and functional regeneration are typical processes of the fetus, gradually lost during postnatal life, and maximally attributed to fetal skin tissue and induced by fetal skin fibroblasts. The latter have been successfully applied to postnatal wounds, with clear advantages compared with autologous dermis grafts or adult fibroblast applications. Our goal was to functionally identify and uncover key factors and mechanisms through the analysis of secretomes, the principal players in all cell therapies based on mesenchymal stromal cells (MSCs). Cell secretomes also putatively mediate skin regenerative effects achieved in clinical applications of fetal skin fibroblasts. An innovative and unbiased approach of comparative and quantitative proteomics of cell conditioned media enabled us to gain knowledge of key molecules and processes from a translational perspective. Using banks of fetal and adult skin fibroblasts that we previously characterized as being MSCs, we discovered secretome changes by identification and comparative quantification, distinguishing secretome signatures of fetal skin MSCs putatively relevant for therapeutic microenvironment modulation. The uncovered proteins can trigger, directly and by modulation of extracellular matrix, angiogenesis, thus highlighting its key role towards scarless wound healing. The angiogenic trigger was functionally validated and corroborated in vitro, with fetal skin MSC secretomes stabilizing and inducing the formation of capillary-like networks by endothelial cells and fetal liver MSCs, respectively. Our approach and our results may aid in the development of cell-based and cell-free products for skin regeneration in acute or chronic injury, and also for wound healing in the regeneration of other tissues. Copyright © 2017 John Wiley & Sons, Ltd.

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Human fetal bone cells in delivery systems for bone engineering

Tenório

Scaletta

Jaccoud

et al. 2011

J Tissue Eng Regen Med

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The aim of this study was to culture human fetal bone cells (dedicated cell banks of fetal bone derived from 14 week gestation femurs) within both hyaluronic acid gel and collagen foam, to compare the biocompatibility of both matrices as potential delivery systems for bone engineering and particularly for oral application. Fetal bone cell banks were prepared from one organ donation and cells were cultured for up to 4 weeks within hyaluronic acid (Mesolis ® ) and collagen foams (TissueFleece ® ). Cell survival and differentiation were assessed by cell proliferation assays and histology of frozen sections stained with Giemsa, von Kossa and ALP at 1, 2 and 4 weeks of culture. Within both materials, fetal bone cells could proliferate in three-dimensional structure at ∼70% capacity compared to monolayer culture. In addition, these cells were positive for ALP and von Kossa staining, indicating cellular differentiation and matrix production. Collagen foam provides a better structure for fetal bone cell delivery if cavity filling is necessary and hydrogels would permit an injectable technique for difficult to treat areas. In all, there was high biocompatibility, cellular differentiation and matrix deposition seen in both matrices by fetal bone cells, allowing for easy cell delivery for bone stimulation in vivo.

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Development, Characterization, and Use of a Fetal Skin Cell Bank for Tissue Engineering in Wound Healing

Cited by 72 publications

References 13 publications

Human muscular fetal cells: a potential cell source for muscular therapies

Human muscular fetal cells: a potential cell source for muscular therapies

Unbiased and quantitative proteomics reveals highly increased angiogenesis induction by the secretome of mesenchymal stromal cells isolated from fetal rather than adult skin

Human fetal bone cells in delivery systems for bone engineering

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