Development and validation of ultra‐high performance supercritical fluid chromatography method for quantitative determination of six compounds in Guizhi Fuling capsule and tablet samples

Li, Bao Qiong; Li, Xin Kang; Lin, Yuan; Li, Ze Ying; Zhang, Xiang‐Zhi; Feng, Na; Ma, Ai‐Jun; Chen, Chao Yang; Tan, Lin Fan

doi:10.1002/jssc.202100181

Cited by 3 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…That process is highly efficient, simple, and with no need for additional instrumentation, sweeping-MEKC has become an effective technique to analyze the same in various matrices. [12][13][14]…”

Section: Sweeping-mekcmentioning

confidence: 99%

Capillary electrophoresis: Recent advancements and applications of micellar electrokinetic capillary chromatography

Navneesh,

Das Gupta,

Kurmi

et al. 2023

Pharmaspire

View full text Add to dashboard Cite

The article discusses capillary electrophoresis (CE) as an advanced technique used for the separation and detection of various pharmaceutical drugs. CE involves the application of high voltages across buffer-filled capillaries to produce separation based on various separation theories, including capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), capillary gel electrophoresis, and capillary isoelectric focusing. While traditional CZE is not suitable for the separation of neutral substances, MEKC was developed by Shigeru Terabe in the early 1990s to expand the use of CE to neutral analytes that cannot be separated using straightforward free solution CE. MEKC employs an ionic micellar solution that interacts with the analytes through partitioning processes like a chromatographic technique. To create a pseudostationary phase, a surfactant such as sodium dodecyl sulfate (SDS) is added to the buffer solution at a concentration higher than its critical micellar concentration. The anionic SDS micelles are electrostatically drawn towards the anode, while the electro-osmotic flow carries the bulk solution toward the negative electrode due to the negative charge on the inside surface of the silica capillaries. When a neutral analyte is introduced into the micellar solution, a portion is integrated into the micelle, while the remaining fraction of the analyte migrates with the electroosmotic velocity. Separation depends on the individual partitioning equilibrium of the various analytes between the micellar and the aqueous phase. The bigger percentage of analyte dispersed inside the micelle, the slower it will travel. This article provides an in-depth understanding of the separation principle and the mechanism involved in MEKC, highlighting its usefulness in separating neutral analytes that cannot be separated using traditional CZE.

show abstract

Section: Sweeping-mekcmentioning

confidence: 99%

Capillary electrophoresis: Recent advancements and applications of micellar electrokinetic capillary chromatography

Navneesh,

Das Gupta,

Kurmi

et al. 2023

Pharmaspire

View full text Add to dashboard Cite

show abstract

“…These capsules have passed the review of the US FDA. A phase II clinical trial (NCT01588236) of Guizhi Fuling Capsules for primary dysmenorrhea has been completed by the US FDA, which found them to be efficacious and safe (77).…”

Section: Chinese Patent Medicines For Gynecological Diseasesmentioning

confidence: 99%

Recent advances in Chinese patent medicines entering the international market

Zhang

Pei

Cai

et al. 2022

DD&T

View full text Add to dashboard Cite

Chinese patent medicines, global acceptance, respiratory infectious diseases, cardiovascular and cerebrovascular diseases, cancers, gynecological diseases As an indispensable part of Traditional Chinese medicine (TCM), Chinese patent medicines have played an important role in preventing and treating diseases in China. Since they are easy to use, easy to store, and cost-effective, Chinese patent medicines have been generally accepted and widely used in Chinese clinical practice as a vital medical resource. In recent years, as TCM has developed and it has been accepted around the world, many Chinese patent medicine companies have gained international market access and successfully registered several Chinese patent medicines as over-thecounter (OTC) or prescription drugs in regions and countries that primarily use Western medicine such as the EU, Russia, Canada, Singapore, and Vietnam. Moreover, several Chinese patent medicines have been obtained the US Food and Drug Administration (FDA) approval conducting phase II or III clinical trials in the US. The current work has focused on several Chinese patent medicines that have been successfully registered or that have been submitted for registration abroad. Summarized here are recent advances in the efficacy and molecular mechanisms of these Chinese patent medicines to treat respiratory infectious diseases (Lianhua Qingwen capsules, Jinhua Qinggan granules, and Shufeng Jiedu Capsules), cardiovascular and cerebrovascular diseases (Compound Danshen Dripping Pills, Huatuo Zaizao pills, and Tongxinluo Capsules), cancers (a Kanglaite injection and a Shenqi Fuzheng Injection), and gynecological diseases (Guizhi Fuling Capsules). The hope is that this review will contribute to a better understanding of Chinese patent medicines by people around the world.

show abstract

“…In recent years, UHPSFC has been used more and more widely and is often used for the determination of various compounds [8]. As a powerful and traditional separation technology, supercritical fluid chromatography uses CO 2 as a mobile phase, which has the characteristics of green and environmental protection.…”

Section: Introductionmentioning

confidence: 99%

An alternative strategy based on ultra‐high‐performance supercritical fluid chromatography for full monosaccharide compositional analysis of polysaccharides in Schisandra chinensis fruits

Gao

Liang

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

Due to green and environment‐friendly characteristics, ultra‐high‐performance supercritical fluid chromatography has been widely used in analytical fields in recent years, but until now few reports are available for monosaccharide compositional analysis of macromolecule polysaccharides. In this study, an ultra‐high‐performance supercritical fluid chromatography technology with an unusual binary modifier is used to determine the monosaccharide compositions of natural polysaccharides. Each carbohydrate herein is simultaneously labeled as 1‐pheny‐3‐methyl‐5‐pyrazolone and acetyl‐derivative via pre‐column derivatizations aiming to increase UV absorption sensitivity and decrease water solubility. Ten common monosaccharides are fully separated and detected on ultra‐high‐performance supercritical fluid chromatography combined with a photo‐diode array detector by systematic optimization of multiple relevant parameters, for example, column stationary phases, organic modifiers, additives, flow rates, and so on. Compared with carbon dioxide as a mobile phase, the addition of a binary modifier increases the resolution of analytes. Additionally, this method has the advantages of small consumption of organic solvent, safety, and being environmental‐friendly. It has been successfully applied for full monosaccharide compositional analysis of heteropolysaccharides from Schisandra chinensis fruits. To sum up, a new alternative approach is provided for monosaccharide compositional analysis of natural polysaccharides.

show abstract

Development and validation of ultra‐high performance supercritical fluid chromatography method for quantitative determination of six compounds in Guizhi Fuling capsule and tablet samples

Cited by 3 publications

References 24 publications

Capillary electrophoresis: Recent advancements and applications of micellar electrokinetic capillary chromatography

Capillary electrophoresis: Recent advancements and applications of micellar electrokinetic capillary chromatography

Recent advances in Chinese patent medicines entering the international market

An alternative strategy based on ultra‐high‐performance supercritical fluid chromatography for full monosaccharide compositional analysis of polysaccharides in Schisandra chinensis fruits

Contact Info

Product

Resources

About