Development and validation of Triticum phytobiological method as an alternative procedure for investigating in vivo acute toxicity on mice

Abstract

Ştefănescu, Emil; Cristea, Aurelia N.; Chiriță, Cornel; Olaru, Octavian; Anghel, Adriana; and Dinu, Mihaela (2017) "Development and validation of Triticum phytobiological method as an alternative procedure for investigating in vivo acute toxicity on mice," Journal of Mind and Medical Sciences: Vol. 4 Keywords: bioethics, triticum phytobiological method, alternative method, acute toxicity, mice AbstractThe goal of this study was to validate an alternative method for determining in vivo acute toxicity using vegetal material instead of laboratory animals, starting from the phytobiological method known also as the Triticum technique. We set out to demonstrate that vegetal cells have similar sensitivity to some toxic agents as animal cells, in which case a statistical correlation could be established. A series of new compounds synthesized by the Romanian National Institute for Chemical Pharmaceutical Research and Development as potential β3 adrenergic receptors agonists were tested for their acute toxicity using classic animal exposure models, before investigating possible anti-diabetic and anti-obesity effects. We then determined whether similar conclusions might be reached exposing vegetal material to the same agents. We successfully demonstrated that plants are affected in a very similar way as animals when exposed to some potentially toxic agents, providing new possibilities for ending unethical animal experiments.Acne conglobata is a rare, severe form of acne vulgaris characterized by the presence of comedones, papules, pustules, nodules and sometimes hematic or meliceric crusts, located on the face, trunk, neck, arms and buttocks.

Experimental Pharmacological Research Regarding the Effect of Some Newly Synthesized Β-Phenylethyl Amines on the Modified Parameters of the Lipid Metabolism

“…New potential β 3 adrenergic receptors agonists are being developed and investigated all over the world [2,23]. Although at present several in vitro methods are available for preclinical trials [17] animal testing remains an essential step for introducing new drugs in therapy. We previously reported the synthesis and development of some new compounds that have a common βphenylethylamine structure and different substituted radicals, which give each substance a different affinity for β 3 adrenergic receptors and a different diffusion level in various body compartments [1,3,12].…”