Development and validation of the liquid chromatographic method for simultaneous estimation of metformin, pioglitazone, and glimepiride in pharmaceutical dosage forms

Pandit, Vinay; Pai, Roopa S; Devi, K; Singh, Gurinder; Narayana, Satya; Suresh, Sarasija

doi:10.4103/2229-4708.97707

Cited by 20 publications

(9 citation statements)

References 8 publications

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“…The kinetic study was carried out for the two test compounds (C25 and C34) and one standard pioglitazone (Fujita et al, 2003;Pandit et al, 2012). The study was conducted in Wister rats weighing between 200-and 250 g. All test substances were given as Sod CMC suspension orally at 10 mg/kg.…”

Section: Pharmacokinetic Study Of Selected Glitazonesmentioning

confidence: 99%

Novel glitazone attenuates rotenone-induced toxicity in mouse model of Parkinson’s disease

Shetty¹,

Krishna²,

Kumar³

et al. 2021

J Appl Pharm Sci

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Parkinson's disease (PD) is the second most common neurodegenerative disorder, and there are no drugs that will directly tackle the inflammatory component of PD. In the present study, we assessed the novel glitazone for reversal of rotenone-induced toxicity in experimental mouse model. The 14 virtual glitazone compounds were subjected to molecular docking study for target protein 3CS8; among these, compound C25 and C34 have shown better binding activity. Pharmacokinetics studies were conducted for the above compounds in rat model to chose best one for further toxicity and efficacy studies. The compound C25 showed better kinetic profile when compared to C34 and better t 1/2 when compared to the standard pioglitazone. Compound C25 then tested for acute toxicity by OECD guideline 423 and evaluated for its neuroprotective activity in mouse model. The activity of the compound was assessed by the behavioral parameters on weekly intervals during the study period and estimated the antioxidant level in the brain homogenate. The compound has shown good activity dose-dependently; however, further research is required to confirm its activity and to support our hypothesis.

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Section: Pharmacokinetic Study Of Selected Glitazonesmentioning

confidence: 99%

Novel glitazone attenuates rotenone-induced toxicity in mouse model of Parkinson’s disease

Shetty¹,

Krishna²,

Kumar³

et al. 2021

J Appl Pharm Sci

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“…High-Performance Liquid Chromatography, Metformin hydrochloride, Teneligliptin hydrobromide, Stability indicating assay, Reverse phase chromatography Correspondence to Author: Dr. Jui Chakraborty RP-HPLC methods for only metformin or metformin hydrochloride with other drugs like linagliptin, glimepirde, and pioglitazone are also reported [7][8][9] while some HPLC methods for metformin hydrochloride with teneligliptin hydrobromide are also available [10][11][12] . The proposed method offers several advantages in terms of simple sample preparation technique with a very accurate, precise, and robust chromatographic system.…”

Section: Fig 2: Chemical Structure Of Teneligliptin Abstractmentioning

confidence: 99%

Untitled

2020

IJPSR

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A simple, rapid, accurate, robust, and specific HPLC method was developed for the assay of metformin hydrochloride and teneligliptin hydrobromide from the oral tablet formulations. Stabilityindicating reverse-phase chromatographic method was developed on a RP C18 column (250 mm × 4.6 mm, 5µm) using a mixture of 20 mM ammonium acetate of pH 5.5 and methanol in the ratio 50:50 v/v as mobile phase in an isocratic mode of elution at a flow rate of 1.0 ml/min at 35 ºC with a load of 20 µl. The detection was carried out at 255 nm. The method was validated with respect to linearity, robustness, precision, accuracy, specificity & stability as per ICH guidelines. The method produced excellent separation with good linear correlation coefficients (≥ 0.999) for both the components. The proposed method could be successfully applied for the assay of metformin hydrochloride and teneligliptin hydrobromide from tablet formulations. INTRODUCTION: Metformin hydrochloride is chemically 1,1-Dimethylbiguanide hydrochloride 1 while teneligliptin hydrobromide hydrate is a Dipeptidyl peptidase-4 (DPP-4) inhibitor and chemically it is {(2S,4S)-4-[4-(3-methyl-1-phenyl-1H-pyrazol-5-yl) piperazin-1-yl] pyrrolidin-2-yl} (1,3-thiazolidin-3-yl) methanone hemipentahydrobromide hydrate. Teneligliptin is used in the treatment of type-2 diabetes mellitus 2. The chemical structures of both drugs metformin hydrochloride and teneligliptin hydrobromide are shown in Fig. 1 and Fig. 2, respectively.

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“…Every stressed sample was analyzed by improved RP-UFLC method. The degradation of data for glimepiride was shown below [11][12][13][14][15][16] .…”

Section: Forced Degradation Studiesmentioning

confidence: 99%

Untitled

2019

IJPSR

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The study aimed to develop a specific, exact, selective, precise and accurate Reversed-Phase Stability Indicating Ultra-fast Liquid Chromatography (RP-UFLC) strategy is created and validated for the determination of glimepiride in the tablet dosage form. The method showed an adequate separation for glimepiride from their degradation products. The optimum separation was achieved by using 250 mm × 4.6 mm C18 column (5 μm) with a mixture of potassium dihydrogen orthophosphate adjusted to pH 4.6 using (orthophosphoric acid) and acetonitrile in the ratio of 75:25 at a flow rate of 1 ml/min. The detection was carried out at 236 nm, and the retention time was found to be 5.9 min. Linearity was observed (correlation coefficient r 2 0.9989). % R.S.D was found to be less than 2%. Accuracy of the method found to be in the range of 98.0 to 102.00 (% w/w). The above method was validated concerning system suitability, linearity, precision, the limit of detection (LOD) and limit of quantification (LOQ), accuracy (recovery) and robustness according to ICH guidelines. The linearity of the above methods was found to be 2-10 µg/ml for glimepiride and force degradation were carried out. Hence these methods can be used for routine analysis in quality control laboratories.

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Development and validation of the liquid chromatographic method for simultaneous estimation of metformin, pioglitazone, and glimepiride in pharmaceutical dosage forms

Cited by 20 publications

References 8 publications

Novel glitazone attenuates rotenone-induced toxicity in mouse model of Parkinson’s disease

Novel glitazone attenuates rotenone-induced toxicity in mouse model of Parkinson’s disease

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