Development and validation of stability-indicating UPLC method for the determination of lafutidine and its impurities in bulk and pharmaceutical dosage form

Abstract

The current study was designed to give more knowledge and helping to exploit the leaves of the plants L. stoechas collected in Tlemcen region in the west of Algeria by determining the anti-corrosive activity of its essential oil. The inhibitory efficiency of the essential oil of the plant obtained by hydrodistillation and characterized by GC and GC/MS was studied using gravimetric and electrochemical methods (polarization curves and electrochemical impedance). The effect of temperature on the corrosion behavior of the steel and the inhibitory efficiency was studied in a temperature range of 303-323 K at 2 g/L. The surface morphology of the samples immersed in 1 M HCl for 24 h, before and after adding inhibitor at (2 g/L at 30 °C) was analyzed by scanning electron microscope (SEM) Quanta 250 with tungsten filament from the company FEI. Results show that the addition of the essential oil of the plant to the medium induces a diminish in the rate of corrosion and augmentation in the inhibitory efficiency of the oil. We established that the inhibition efficiency increase with concentration of the essential oil of lavender to attain 76.19% at 2 g/L. Polarization curves revealed that lavender oil react as a mixed-type inhibitor. EIS spectra exhibit one capacitive loop and confirm the inhibitive ability, and the changes in impedance parameters were indicative of adsorption of essential oil of lavender on the metal surface. The thermodynamic parameters indicate that the adsorption of the molecules of the oil takes place according to the Langmuir isotherm in the corrosive medium studied and that they are physisorbed on the metal surface. The analysis of the surface by electron microscopy demonstrates the absence of surface attack patterns in the presence of the oil. The results obtained from different tested techniques were in good agreement.

Bio-analytical Method Development for Pharmacokinetic Study of Novel Lafutidine Formulation in Rabbit Plasma Using RP-HPLC

“…The drug has poor bioavailability due to its short half-life and selective absorption, which make it appropriate drug candidate for gastroretentive raft system. Many stability indicating methods have been developed for the estimation of lafutidine in bulk formulations, such as HPTLC, 10 RP-HPLC, 11 UPLC, 12 and UV spectroscopy. 13 Lafutidine estimation in human plasma, such as LC-ESI-MS, 14 and HPLC-MS 15 has been reported.…”

Analytical Method Development and Validation of Ondansetron and Telmisartan in Tablet Dosage Form by RP-UPLC Method

“…Different modes of liquid chromatography have developed for analyses of a wide range of ingredients in several kinds of matrices. In normal-phase fluid chromatography, the fixed stage is a polar great adsorbent according to specks of silica gel, carbon or alumina 15 . The movable stage is made up of non polar, natural solvents (dehydrated), for instance methanol, ethanol, 2 propanol, acetonitrile, co2 tetrachloride or maybe hexane.…”

Forced degradation of lafutidine and characterization of its non-volatile and volatile degradation products using LC-MS/TOF, LC-MSn and HS-GC-MS

“…The latter causes inhibition of parietal cells, resulting in suppression of gastric acid secretion. 3 Several methods have been reported for analysis of lafutidine employing HPLC, [4][5][6][7] UPLC, 8 LC-ESI-MS, 9 HPTLC, 10,11 UV spectrophotometry, 12,13 and spectrofluorimetry. 14 To the best of our knowledge, no information is available on characterization of its degradation products (DPs).…”