Development and Validation of Stability Indicating Derivative Spectrophotometric Methods for Determination of Duloxetine Hydrochloride

Chadha, Renu; Bali, Alka

doi:10.9734/bjpr/2015/17814

Cited by 5 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several reports in literature based on the application of reverse phase chromatographic methods [7]- [12] or potentiometric methods [13] Spectrophotometric methods have also been investigated and these include the application of more sensitive derivative spectrophotometry as well [14] [15] [16] (LOD = 9.0 × 10 −6 and LOQ = 1.0 × 10 −5 mol L −1 ). A spectrofluorimetric method has been reported for DXT.HCl based on enhancement effect of cationic surfactants on the native fluorescence intensity in an alkaline medium [17].…”

Section: Introductionmentioning

confidence: 99%

Trace Determination of Duloxetine HCl in Formulation and Spiked Human Serum at a Carbon Paste Electrode

Hassanein¹,

Moharram²,

Oraiby³

et al. 2017

AJAC

View full text Add to dashboard Cite

The electrochemical behavior of duloxetine HCl (DXT.HCl) at a carbon paste electrode (CPE) was achieved by cyclic voltammetry and a mechanism of its oxidation was reported and illustrated. A sensitive linear sweep and square-wave adsorptive anodic stripping voltammetry methods were described for trace determination of DXT.HCl. Introduction: DXT.HCl, N-methyl-3-(nap-thalen-1-yloxy)-3-(thiophene-2-yl) propan-1-amine hydrochloride is a selective serotonin-norepinephrine reuptake inhibitor (SNRI) originally developed as an antidepressant and is currently recommended for maintenance treatment of major depressive disorder. Methods: Electrochemical behavior was studied using cyclic voltammetric method, and the analytical application was studied using linear sweep and square wave voltammetric methods. Solution pH has been measured by pH meter. Results: The process on the surface of carbon paste electrode was found to be irreversible and diffusion-controlled. The number of proton and electron transferred were calculated. Possible reaction mechanism taking place on the surface of electrode was proposed. Calibration plot constructed using square wave voltammetric technique was used for quantitative analysis in pharmaceutical and human serum samples. Limit of detection (LOD) and limit of quantification (LOQ) were calculated. Conclusions: In the present work, we described the electrochemical behavior of DXT.HCl drug. Two precise linear sweep and square wave adsorptive anodic stripping voltammetry methods have been described for its trace quantitation in pharmaceutical formulation and human serum. The method shows the development of a sensor for selective and sensitive determination of DXT.HCl.How to cite this paper: Hassanein, A.M.,

show abstract

Section: Introductionmentioning

confidence: 99%

Trace Determination of Duloxetine HCl in Formulation and Spiked Human Serum at a Carbon Paste Electrode

Hassanein¹,

Moharram²,

Oraiby³

et al. 2017

AJAC

View full text Add to dashboard Cite

show abstract

“…However, most of the published papers for DLX assay use spectrophotometric techniques that suffer from low sensitivity and comprise an extraction step, which renders the procedure tedious and timeconsuming [6][7][8][9][10][11]. Other techniques used HPLC, which needs ultra-pure solvents, sample pretreatment, expensive detectors and trained personnel.…”

Section: Introductionmentioning

confidence: 99%

“…The published methods for DLX involve spectrophotometry [6][7][8][9][10][11], spectrofluorimetric approaches based on native fluorescence property [12,13] or derivatization by NBD-Cl to give fluorescent product measured at 523 nm [14]. Thin-layer chromatography was also reported for the detection of DLX [15,16], as were electrochemical methods [17,18], gas chromatography [19] and high-performance liquid chromatography (HPLC) [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Two facile approaches based on association complex with erythrosine-B for nano-level analysis of duloxetine: application to content uniformity

2021

View full text Add to dashboard Cite

Duloxetine is an antidepressant that exhibits its action by preventing the reuptake of serotonin and norepinephrine by neurons. In this analytical study, we developed two facile, sensitive methods for duloxetine analysis. Both methods rely on the formation of binary association complex between erythrosine-B and duloxetine in an acidic medium using spectrofluorimetric and resonance Rayleigh scattering (RRS) techniques. Spectrofluorimetric method simply uses the quenching property of the formed complex on the native fluorescence of erythrosine-B at an emission wavelength of 557.2 nm ( λ ex = 528.6), while RRS is based on detecting the enhancement in the RRS signal at 357.2 nm. The proposed methods have been validated according to the International Conference on Harmonization guidelines. The approaches provide linear assay of duloxetine hydrochloride over 0.1–2.4 µg ml −1 and 0.2–2.0 µg ml −1 for spectrofluorimetric and RRS methods, respectively. Variables affecting methods and complex formation were studied and optimized. The limit of detection values were 0.03 and 0.056 µg ml −1 for spectrofluorimetric and RRS methods, respectively. Both approaches were applied with acceptable results for formulation analysis and evaluation of cymbatex capsule content uniformity.

show abstract

“…4,5 Although DUL is deemed to be one of the main antidepressant drugs, the development of efficient and exact methods for the evaluation of this active compound are of interest. Several analytical methods are implemented in the determination of DUL including potentiometry, [6][7][8] spectrofluorimetry, [9][10][11] spectrophotometry, [12][13][14][15] capillary zone electrophoresis, 16,17 gas chromatography-mass spectrometry, 18 high-performance liquid chromatography, [19][20][21][22][23] liquid chromatography coupled with mass spectrometry, [24][25][26][27][28][29][30][31] high performance thin layer chromatography. 32,33 Although developed methods are applicable with high sensitivity, they are not efficient in the detection of DUL in real time of consumption, requiring sample pretreatment preparations, a longer analysis time and involve the use of expensive apparatuses.…”

mentioning

confidence: 99%

Electrochemical Behavior of Duloxetine Hydrochloride at Au and GC Solid Electrodes: Its Quantitative Determination and Degradation

Lović¹,

Bogdanović²,

Tadić³

et al. 2022

J. Electrochem. Soc.

View full text Add to dashboard Cite

Duloxetine hydrochloride (DUL) is effective in treating depression, and was examined for electroanalytical purposes. The DUL standard was investigated by cyclic voltammetry (CV) and determined using differential pulse voltammetry (DPV) via its electro-oxidation at the Au electrode in 0.05 M NaHCO3. DPV showed a linear dependency of the anodic peak currents vs DUL standard concentrations in the range from 0.1 to 3.33 μg ml−1 with the values of the limit of detection (LOD) and the limit of quantification (LOQ): 0.133 and 0.667 μg ml−1, respectively. Using the constructed and validated calibration curve, the values of unknown DUL concentrations in both Taita® tablets and in human serum spiked with the standard were determined. The number of protons and electrons transferred was calculated and possible reaction mechanisms taking place on the surface of both electrodes were proposed. The Au electrode exhibited a better sensitivity and a wider range of current vs concentration linear dependency for DUL quantitative determination than the GC electrode. The study of DUL degradation showed that at the Au and GC electrodes, after 4.5 h of potential cycling, degradation occurs, giving formaldehyde as a product, which was confirmed by high performance liquid chromatography (HPLC).

show abstract

Development and Validation of Stability Indicating Derivative Spectrophotometric Methods for Determination of Duloxetine Hydrochloride

Cited by 5 publications

References 0 publications

Trace Determination of Duloxetine HCl in Formulation and Spiked Human Serum at a Carbon Paste Electrode

Trace Determination of Duloxetine HCl in Formulation and Spiked Human Serum at a Carbon Paste Electrode

Two facile approaches based on association complex with erythrosine-B for nano-level analysis of duloxetine: application to content uniformity

Electrochemical Behavior of Duloxetine Hydrochloride at Au and GC Solid Electrodes: Its Quantitative Determination and Degradation

Contact Info

Product

Resources

About