Development and Validation of Stability Indicating HPLC Method for Determination of Caffeic Acid, Vitexin and Rosmarinic Acid in Thunbergia laurifolia Leaf Extract

Abstract

Thunbergia laurifolia has been a popular herb used in Thai traditional medicine for detoxification and as antipyretic. It contains rosmarinic acid (RA), caffeic acid (CA) and vitexin as major compounds. In order to control the herbal quality, the stability indicating high-performance liquid chromatography (HPLC) was developed and validated. The stability study of compounds in T. laurifolia leaf extract was investigated. The chromatographic separation was performed using a reversed-phase C18 column and mobile phase consisted of 0.5% acetic acid and methanol using a gradient elution with 1.0 mL/min flow rate. The detection wavelength was set at 330 nm. The method was validated for its linearity, precision, accuracy, limit of detection and limit of quantitation. Forced degradation of three compounds in extract showed that they were stable in oxidative condition, but highly labile under alkaline hydrolytic conditions. All three compounds in T. laurifolia leaf extract were stable at room temperature at least 3 months while a remarkable decrease of RA, vitexin and CA in the extract were found in accelerated condition. This finding could be applied for predicting the storage recommendation and expiry of T. laurifolia extract and its related pharmaceutical products.

Fabrication and characterization of nanoemulsions for encapsulation and delivery of vitexin: antioxidant activity, storage stability and in vitro digestibility

“…No significant changes were observed in the antioxidant activity of nanoemulsions loaded with vitexin during storage for 45 days at all temperatures. This result corresponded to a report that vitexin was stable at room temperature for up to 3 months, with slight decomposition observed at 40 °C 58 . It can be suggested that the formulated nanoemulsion maintained the antioxidant property of vitexin during storage.…”

“…This result corresponded to a report that vitexin was stable at room temperature for up to 3 months, with slight decomposition observed at 40 °C. 58 It can be suggested that the formulated nanoemulsion maintained the antioxidant property of vitexin during storage. The emulsifiers used to fabricate these nanoemulsion systems, Tween 80 and lecithin, also contributed to the antioxidant properties and provided synergistic scavenging of the encapsulated vitexin.…”

StandardizedThunbergia laurifoliaExtract Inhibits PM2.5-Induced Oxidative Stress by Regulating p62–KEAP1–NRF2 Signaling Pathway

“…Chromatography was achieved using Purospher ® RP-18 end-capped HPLC column (250 cm×4.6 mm, 5 μm) from Merck KGaA (Darmstadt, Germany). The mobile phases consisted of 0.5% acetic acid in deionized water and methanol with a gradient elution according to Woottisin et al (16). A constant flow rate of 1.0 ml/min was conducted throughout the analysis with temperature control at 25˚C.…”

Comprehensive study of allergenic tree species: Palynological insights enhanced by HPLC and GC–MS profiling

“…Polyphenols are believed to possess the capacity to scavenge free radicals due to their reducing abilities, as they can serve as donors of hydrogen or electrons. As indicated by Woottisin et al (2020), these extracted phenolic compounds, which are secondary metabolites produced by plants, have various positive effects on human health and are directly associated with their antioxidant properties.…”