Development and validation of stability indicating RP-HPLC method for the estimation of acamprosate calcium in pure and pharmaceutical dosage forms

Rao, Nagasarapu Mallikarjuna; Sankar, D. Gowri

doi:10.5530/rjps.2014.4.6

Cited by 3 publications

(1 citation statement)

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“…[14][15][16][17][18][19][20][21][22][23] Degradation was induced at a concentration of 1000 µg/ml. At different time points, 0.5 ml of degraded solution was diluted with mobile phase to 10 ml(50 µg/ml), pH was adjusted between 3-4 with dil.…”

Section: Stress Degradation Studies Of Azmmentioning

confidence: 99%

Reliable and Sensitive Stability Indicating – Liquid Chromatographic Method for Determination of Azilsartan Medoxomil and Characterization of Common Hydrolytic Degradation Product

Peraman¹,

Dakinedi²,

Kadiri³

et al. 2017

JYP

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Objective: The objective of the proposed method was to develop an accurate, rapid and cost-effective stability indicating a reversed phase high-performance liquid chromatographic method for the determination of azilsartan medoxomil. Materials and Methods: Chromatographic separation of the analyte from its degradation products was achieved on QualisilGold column with a mobile phase composition of Acetonitrile and 0.2% Triethyl amine[pH 3.0], [62:38%v/v] at flow rate was 1.0 ml/min, the eluents were monitored using PDA detector at 248 nm. Results: The method was linear (r 2 = 0.9997) in the concentration range of 20-120 µg/ml with acceptable percentage relative standard deviations for Inter-day (0.22 %) and Intraday (0.31 %) precisions. The result for accuracy study was 99.92-100.29 %. Conclusion: The method has proven specificity to quantify azilsartan medoxomil in presence of its degradation products formed in stress studies. Five major degradation products were found in stress studies, among them impurity-4 was identified and it was also observed that the drug was more susceptible to thermal and photolytic degradations.

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Section: Stress Degradation Studies Of Azmmentioning

confidence: 99%

Reliable and Sensitive Stability Indicating – Liquid Chromatographic Method for Determination of Azilsartan Medoxomil and Characterization of Common Hydrolytic Degradation Product

Peraman¹,

Dakinedi²,

Kadiri³

et al. 2017

JYP

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Rp-HPLC Method Development and Validation for Simultaneous Estimation of Linagliptin and Empagliflozin

Rao¹,

Prasanthi²,

Anusha³

2019

IND. DRU.

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A simple, accurate and precise RP-HPLC method was developed for the simultaneous estimation of the linagliptin and empagliflozin in tablet dosage form. Chromatogram was run through Kromasil 250 x 4.6 mM, 5mM column, mobile phase containing 0.1% o-phosphoric acid buffer and acetonitrile in the ratio of 60:40%v/v was pumped through column at a flow rate of 1 mL/min. The optimized wavelength was 230 nm. Retention times of linagliptin and empagliflozin were found to be 2.759 min and 2.139 min. %RSD of the Linagliptin and Empagliflozin were found to be 0.5 and 0.6 respectively. Percentage assay was obtained as 99.91% and 100.15% for linagliptin and empagliflozin, respectively. LOD, LOQ values obtained for linagliptin and empagliflozin were 0.23 μg/ml and 0.44 μg/mL and 0.70 μg/mL and 1.34 μg/mL, respectively. Thus, the current study showed that the developed RP-HPLC method is sensitive and selective for the estimation of linagliptin and empagliflozin in combined dosage form.

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Development of Liquid Chromatographic Method for Estimation of Acamprosate Calcium and Baclofen Combination Used in Treatment of Neurological Disorders

Jadeja¹,

Patel²,

Shah³

et al. 2021

IND. DRU.

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A specific, accurate, precise, and reproducible liquid chromatographic method has been developed and validated for the estimation of acamprosate calcium and baclofen in combination. The separation was achieved using stationary phase Phenomenex C18 column (150 mm× 4.6 mm.) in isocratic mode, with mobile phase containing 0.05 M potassium dihydrogen orthophosphate buffer (pH 7) : acetonitrile (10:90 V/V), at a flow rate of 1.0 mL/min and effluents were monitored at 210 nm. The retention time of acamprosate calcium and baclofen were found to be 1.9 min and 5.3 min, respectively. The linearity for acamprosate calcium and baclofen were in the range of 2-64 µg/mL and 1.2- 38.4 µg/mL, respectively. The method was validated as per ICH guideline. The recoveries of Acamprosate calcium and baclofen were found in the range of 98.90 - 100.13 % and 98.60 -100.02 %, respectively. The method was successfully applied for the determination of both the drugs in combination.

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Development and validation of stability indicating RP-HPLC method for the estimation of acamprosate calcium in pure and pharmaceutical dosage forms

Cited by 3 publications

References 0 publications

Reliable and Sensitive Stability Indicating – Liquid Chromatographic Method for Determination of Azilsartan Medoxomil and Characterization of Common Hydrolytic Degradation Product

Reliable and Sensitive Stability Indicating – Liquid Chromatographic Method for Determination of Azilsartan Medoxomil and Characterization of Common Hydrolytic Degradation Product

Rp-HPLC Method Development and Validation for Simultaneous Estimation of Linagliptin and Empagliflozin

Development of Liquid Chromatographic Method for Estimation of Acamprosate Calcium and Baclofen Combination Used in Treatment of Neurological Disorders

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