Development and Validation of Spectrophotometric and Pre-column Derivatization HPLC Method for Determination of Famotidine in Pharmaceuticals by Reaction with Sodium Nitroprusside; Application to Combined Tablets

Abstract

Spectrophotometric and HPLC methods for estimation of famotidine in pharmaceutical formulations through derivatization with sodium nitroprusside were developed. The spectrophotometric method is based on measuring the red color formed after the reaction with sodium nitroprusside at 498 nm. The formed product was further determined by HPLC method using C 18 column, mobile phase composed of methanol and 0.05 M phosphate buffer (30:70, v/v) with apparent pH 4, The UV detection was at 498 nm. Both methods were linear covering concentrations of 20-500 µg/mL. The selectivity and the simplicity of the methods allow the successful estimation of famotidine in its pharmaceuticals and in its combined tablets with ibuprofen, domperidone, paracetamol and diclofenac without interferance.

Assay of Famotidine in Pure Solutions and Pharmaceutical Preparatives using the Coated- graphite Electrode as a Detector in the FlA System

“…Famotidine is usually given to the patient 40 mg daily as a therapeutic nap , and Famotidine is completely absorbed by the gastrointestinal tract with a maximum concentration in the plasma about 3 hours after oral administration [3]. The literatures described various methods used to assay famotidine in the form of pharmaceutical preparatives and biological fluids such as spectrophotometric methods [4][5][6][7][8][9], chromatographic methods [10][11][12][13][14], the flow injection analysis method using a spectrophotometry [15], and electrochemical methods [16][17][18].However, most of these techniques were not particularly beneficial for assessing famotidine due to its suffering from many problems such as response time, simplicity, selectivity, efficacy, economic cost, and maximum detection. The flow injection analysis system (FlA) proved that it is an easy and appropriate way to analyze the samples due to the consumption of the reagent , the sample is low, the response time is less , the rate is high in estimating the samples , reproducibility for their results [19,20] and can also be applied to colored and turbid solutions [21].…”

Spectrofluorimetric Determination of Some N. Containing Medicines Using Rhodamine 6G as a Chromogenic Reagent

“…Several analytical techniques have been described for the determination of the above drugs in their pure form and pharmaceutical formulations. These include HPLC [11][12][13][14][15][16][17][18], spectrophotometric [19][20][21][22][23][24][25][26][27][28], conductometric [29], and electrochemical methods [30][31][32][33] were described for the determination of these drugs. Few spectrofluorimetric methods have been reported in the literature for the determination of the studied drugs.…”

Use of a monolithic column for the development and validation of a HPLC method for the determination of famotidine, cimetidine and nizatidine in biological fluids

“…A literature survey retrieved a large number of references reporting analytical methods for the determination of these compounds. Mainly separation techniques such as Gas Chromatography (GC) [2], High Performance Thin Layer Chromatography (HPTLC) [3][4][5][6][7][8][9][10], Capillary Electrophoresis (CE) [11][12][13][14][15][16][17] and High Performance Liquid Chromatography (HPLC) [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] have been used, with the latter being the predominant technique for the determination of these compounds in a variety of matrices, utilizing different methods of sample pretreatment and detection modes. HPLC with ultra-violet (UV) detection, employing reversed phase particle packed columns, is most frequently used for the analysis of these drugs in pharmaceutical preparations and biological fluids.…”