Development and validation of SPE-HPLC method for the determination of carbamazepine and its metabolites carbamazepine epoxide and carbamazepine trans-diol in plasma

Abstract

SPE-HPLC method has been developed and validated for rapid analysis of carbamazepine and its two metabolites carbamazepine epoxide and carbamazepine trans-diol in human plasma. The analysis was performed using C18 Bakerbond-BDC analytical column (250 mm x 4.6 mm i.d., particle size 5 μm). The optimal conditions for the separation were established with the mobile phase acetonitrile - 10 mM phosphate buffer, pH 7.0 (30:70, v/v) at the flow rate of 1.5 mL min-1, temperature 35°C, and UV detection at 210 nm. Total run time was about 8 minutes. SPE procedure for extraction of the analytes from plasma sample was developed using Oasis HLB cartridges and subsequently eluate was injected into the HPLC system for analysis. Afterwards, SPE-HPLC method was subjected to validation. Linearity was obtained over the concentration range of 0.2-25 μg/mL for carbamazepine, carbamazepine epoxide and carbamazepine trans-diol with correlation coefficients higher than 0.995. The method showed good intra-day and inter-day precision with relative standard deviation below 7.96%, while accuracy ranged from 92.09% to 108.5% for all analytes. Finally, the method was successfully applied to analysis of plasma samples of epileptic patients in monotherapy and polytherapy. [Acknowledgments. Projekat Ministarstva nauke Republike Srbije, br. OI 172033]

Hot liquid extrusion assisted drug-cyclodextrin complexation: a novel continuous manufacturing method for solubility and bioavailability enhancement of drugs

“…The amount of CBZ was estimated as per the method reported by Džodić et al [ 36 ] using reverse-phase HPLC. The HPLC system (Shimadzu, Kyoto, Japan) comprised dual piston pumps, an auto sampler, a UV-visible detector, and LC solution software.…”

Ionic Liquid-Based Dispersive Liquid–Liquid Microextraction for the Simultaneous Determination of Carbamazepine and Lamotrigine in Biological Samples

“…The comparison of extraction time of the proposed method with solid-phase microextraction (SPME), 32 liquid-liquid extraction (LLE), 33,34 and solid-phase extraction (SPE) 35 for the extraction of the target analytes indicates that this novel method has a very short equilibrium time comparing to the mentioned methods and the extraction time needed for the proposed method is a few seconds. Quantitative results of the proposed method are better than for SPE 35 and LLE 33,34 methods. Relative standard deviation (RSD%) of the proposed method is better than for SPME 32 and LLE 33 methods.…”

Development and Validation of Carbamazepine Plasma Concentrations Measurement and Its Application on Epilepsy Patients

“…One of the simplest and most accurate methods is highperformance liquid chromatography (HPLC) [1]. Previous studies indicated that HPLC was adequate, effective, suitable, simple, rapid, highly sensitive, also had a good intra-day and inter-day precision and an accuracy of >90% [1][2][3][4][5][6]. Considering many patients also use other AEDs, the physician has to make sure that the measurement of carbamazepine plasma levels is unaffected by the presence of other AED.…”