Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry

Bernert, John T.; Turner, Wayman E.; Pirkle, James L.; Sosnoff, Connie S.; Akins, James R.; Waldrep, Mary K.; Ann, Qinghong; Covey, Thomas R.; Whitfield, W; Gunter, Elaine W.; Miller, Barbara B.; Patterson, Donald G.; Needham, Larry L.; Hannon, W. Harry; Sampson, Eric J.

doi:10.1093/clinchem/43.12.2281

Cited by 310 publications

(101 citation statements)

References 31 publications

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“…We also measured hair and serum cotinine using standard techniques at each home visit. [25][26][27] Lower detection limits for serum and hair cotinine were 0.05 ng/ml and 0.005 ng/mg, respectively. [25][26][27] Lastly, we measured airborne nicotine levels using nicotine dosimeters that were located on the back of the air cleaner machines in the main activity room at the baseline and 6-month visits, and retrieved at the 6-and 12-month visits respectively so that the first dosimeter reflected nicotine exposure from baseline to 6 months and the second 6-12 months.…”

Section: Environmental Exposure Measuresmentioning

confidence: 93%

Environmental exposures, nitric oxide synthase genes, and exhaled nitric oxide in asthmatic children

Spanier

Kahn

Hornung

et al. 2009

Pediatric Pulmonology

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Exhaled nitric oxide (FeNO), a measure of airway inflammation, is being explored as a tool to guide asthma management in children. Investigators have identified associations of genetic polymorphisms in nitric oxide synthase genes (NOS1 and NOS3) with FeNO levels; however, none have explored whether these polymorphisms modify the relationship of environmental exposures with FeNO. The objective of this project was to evaluate the association of NOS polymorphisms and environmental exposures with FeNO levels among children with asthma. We conducted a 12‐month prospective cohort study of 225 tobacco‐smoke exposed children (6–12 years) with doctor‐diagnosed asthma. We assessed environmental exposures (tobacco, indoor allergens, & airborne particulates), polymorphisms in NOS1 (an intronic AAT tandem repeat) and NOS3 (G894T), and FeNO levels. There was no association of NOS1 or NOS3 polymorphisms with FeNO levels. There were no significant interactions of environmental exposures and the NOS1 polymorphism with FeNO levels. In contrast, there was an interaction of the NOS3 polymorphism and airborne nicotine concentration with FeNO levels (P = 0.01). Among GG genotype individuals, nicotine exposure did not affect FeNO levels; however, among individuals with at least one T allele, higher nicotine exposure was associated with lower FeNO levels (approximately 5 ppb decrease from the lowest to the highest quartile). We conclude that genetic differences may explain some of the conflicting results in studies of the effects of tobacco smoke exposure on FeNO levels and may make FeNO interpretation difficult for a subset of children with asthma. Pediatr Pulmonol. 2009; 44:812–819. © 2009 Wiley‐Liss, Inc.

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Section: Environmental Exposure Measuresmentioning

confidence: 93%

Environmental exposures, nitric oxide synthase genes, and exhaled nitric oxide in asthmatic children

Spanier

Kahn

Hornung

et al. 2009

Pediatric Pulmonology

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“…Urine samples were immediately put on ice after collection, frozen at À208C within 3 hr and transferred to À708C within 24 hr. The cotinine value reported in this study is the sum of cotinine and 3 0hydroxycotinine as determined by gas chromatographymass spectrometry 17 at the University of California at San Francisco Clinical Pharmacology Laboratory. The limits of quantitation for these assays were 0.10 and 0.05 ng/ml, respectively.…”

Section: Methodsmentioning

confidence: 99%

Effect of secondhand cigarette smoke, RSV bronchiolitis and parental asthma on urinary cysteinyl LTE4

Kott

Salt

McDonald

et al. 2008

Pediatric Pulmonology

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Cysteinyl leukotrienes promote airway inflammation, bronchoconstriction and mucus hypersecretion. Cigarette smoking and respiratory syncytial virus (RSV) bronchiolitis are known to increase urinary cysteinyl leukotriene E4 (uLTE4), the end product of the cysteinyl leukotriene biosynthetic pathway. We tested the following hypotheses: (1) Secondhand smoke (SHS) exposure increases uLTE4 in well infants and in those hospitalized for RSV bronchiolitis; (2) Length of hospital stay for those with RSV bronchiolitis correlates with uLTE4; and (3) Infants with parent(s) with asthma will have higher uLTE4. Parental asthma for infants hospitalized with RSV bronchiolitis (n = 79) and Well babies (n = 31) was determined by questionnaire. Urine was analyzed for LTE4, cotinine, and creatinine. SHS exposure was determined by cotinine to creatinine ratio. Chi square, or t-tests were used to determine significant differences between two groups. A three-way analysis of variance compared the effects of SHS exposure and parental asthma on uLTE4 in Well versus RSV babies. Independent variables predicting length of hospital stay were determined by stepwise multiple regression. High SHS exposure and RSV significantly increased uLTE4. The SHS induced increase in uLTE4 was seen in infants with no parental asthma but not in those with parental asthma. Length of hospital stay positively correlated with uLTE4. We concluded that SHS exposure may increase the severity of bronchiolitis in RSV-infected infants by enhancing production of cysLTs in infants with no parental asthma.

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“…This article has been contributed to by US Government employees and their work is in the public domain in the USA Exposure to environmental tobacco smoke was obtained through the measurement of serum cotinine, a metabolite of nicotine, captures both passive and active exposures as well as the actual amount smoked. We categorized cotinine level as unexposed: <0.35 ng/mL, passively exposed: 0.35-9.99 ng/mL, and actively exposed: ≥10 ng/mL (Bernert et al, 1997).…”

Section: Assessment Of Additional Covariatesmentioning

confidence: 99%

Cross‐sectional association between physical activity and serum testosterone levels in US men: results from NHANES 1999–2004

et al. 2016

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Background Testosterone levels and physical activity (PA) each play important roles in men’s health, but the relationship between the two remains unclear. Methods We evaluated the cross-sectional association between self-reported total PA and serum testosterone levels in 738 men (mean age 42.4 yrs, range 20-≥85yrs) who participated in NHANES 1999–2004. We compared geometric mean testosterone concentrations measured by RIA and calculated the odds ratio (OR) of having low or low normal testosterone (≤3.46 ng/mL) across tertiles of total PA in all men, and men stratified by age (20–49, ≥ 50 years), and obesity status (BMI <30, ≥ 30 kg/m2). Results The geometric mean testosterone concentration was 5.31 ng/mL; 18.6% of the men had low or low normal serum testosterone levels. PA tertiles were not associated with testosterone levels overall, or when stratified by age or obesity status. Similarly, there was no association between PA tertiles and the odds of low or low normal testosterone, overall or by age. However, among non-obese men, those in the highest PA tertile were significantly less likely to have low or low normal testosterone than those in the lowest tertile (OR 0.50; 95% CI = 0.26–0.95); there was no association among obese men. Conclusions Greater PA was not associated with testosterone levels, but may be associated with a reduced odds of low or low normal testosterone in non-obese men, but not in obese men.

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Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry

Cited by 310 publications

References 31 publications

Environmental exposures, nitric oxide synthase genes, and exhaled nitric oxide in asthmatic children

Environmental exposures, nitric oxide synthase genes, and exhaled nitric oxide in asthmatic children

Effect of secondhand cigarette smoke, RSV bronchiolitis and parental asthma on urinary cysteinyl LTE4

Cross‐sectional association between physical activity and serum testosterone levels in US men: results from NHANES 1999–2004

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