Development and Validation of RP-HPLC Method for Simultaneous Estimation of Naproxen and Esomeprazole in Pharmaceutical Dosage Form

Gali, Haritha; Yerragunta, Vishwanadham

doi:10.5958/0974-4150.2016.00055.9

Cited by 7 publications

(4 citation statements)

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“…The corresponding chromatograms were recorded and the area of each peak for ESO was measured at 302 nm. Each sample solution was chromatographed in triplicate and the mean peak area for ESO was calculated (13) .…”

Section: Calibration Curve Of Esomeprazolementioning

confidence: 99%

Development and validation of a high-performance liquid chromatography method for the estimation of esomeprazole in bulk and tablet dosage form

Chorge,

Wankhede,

Kandekar

et al. 2023

Ars Pharm

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Introduction: An accurate, simple, precise, rapid, economic and reproducible reverse-phase high-performance liquid chromatography method was developed and validated for the estimation of Esomeprazole (ESO) in bulk and tablet dosage form. Method: The separation was carried out on Finepak SIL C18T-5 column (250 × 4.6 mm, 5.0 µm i. d.) using potassium dihydrogen phosphate buffer (0.025M): ACN (20:80 v/v) and at a flow rate of 1.0 mL/min. using UV detector at 302 nm with a run time of 10 min. The method was validated for accuracy for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. Results: The standard calibration curve was linear with R2 = 0.995. LOD and LOQ obtained for esomeprazole were 0.0001 and 0.0004 µg/mL respectively. The method was found robust for possible changes. Results of analysis of other parameters were also tested and validated as per ICH guidelines and recovery studies confirmed the accuracy of the proposed method. validation studies showed that the developed HPLC method is simple, reproducible, rapid, precise and reliable. The high recovery and low relative standard deviation confirm the suitability of the developed method for the determination of esomeprazole in the tablet dosage form. Conclusion: This method may be used as a more convenient and efficient option for the analysis of esomeprazole to establish the quality of the substance during routine analysis with consistent and reproducible results.

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Section: Calibration Curve Of Esomeprazolementioning

confidence: 99%

Development and validation of a high-performance liquid chromatography method for the estimation of esomeprazole in bulk and tablet dosage form

Chorge,

Wankhede,

Kandekar

et al. 2023

Ars Pharm

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show abstract

“…The solution was iltered through 0.45µ Whatman ilter. From this 1µl of Sample solution was applied on the pre-coated silica gel 60F 254 plate and from the peak area obtained, the amount of Lisinopril in formulation was calculated using the calibration graph [12,13].…”

Section: Preparation Of Sample Solutionmentioning

confidence: 99%

Development of analytical method and validation for determination of Lisinopril dihydrate in bulk drug and dosage form using HPTLC method

Khan¹,

Khan²,

Sadeque³

et al. 2018

jibps

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A simple, reproducible and efficient High Performance Thin Layer Chromatography method was developed for Lisinopril dihydrate in bulk drug and dosage form. A constant application rate of 0.1 ml/s with nitrogen aspirator was used, and the space between two bands was 6 mm. The slit dimension was 5 × 0.45 mm, and the scanning speed was 10 mm/s. The mobile phase consisted of n-butanol: methanol: ammonia in the ratio of 3.0: 1.0: 1.0 (v/v/v). The retention time (min) and linearity range (μl) for Lisinopril was (0.20) and (1-5) respectively. The method so developed was validated for its accuracy and precision. The LOD and LOQ were found to be 0.050237 and 0.152233 for Lisinopril respectively. The accuracy was found to be 98.88%. The developed method was found to be accurate, precise and selective for determination of Lisinopril in bulk and dosage form.

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“…The solution containing 10µg/ml of lisinopril was injected into the column six times. The average peak area of the drug was computed from the chromatograms and the amount of the drug present in the tablet dosage form was calculated by using the regression equation obtained for the pure drug [11][12][13].…”

Section: Estimation Of Lisinopril In Tablet Dosage Formsmentioning

confidence: 99%

Development and validation for determination of lisinopril dihydrate in bulk drug and formulation using RP-HPLC method

Zaheer¹,

Khan²,

Sadeque³

et al. 2018

jibps

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A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for Lisinopril in bulk drug and formulation. A column having 150 × 4.6 mm in isocratic mode with mobile phase containing acetonitrile: phosphate buffer (70:30; adjusted to pH 3.0) was used. The flow rate was 0.8 ml/min and effluent was monitored at 216 nm. The retention time (min) and linearity range (μg/ml) for Lisinopril was (1.510) and (10-35). The developed method was found to be accurate, precise and selective for determination of Lisinopril in bulk and formulation.

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Development and Validation of RP-HPLC Method for Simultaneous Estimation of Naproxen and Esomeprazole in Pharmaceutical Dosage Form

Cited by 7 publications

References 0 publications

Development and validation of a high-performance liquid chromatography method for the estimation of esomeprazole in bulk and tablet dosage form

Development and validation of a high-performance liquid chromatography method for the estimation of esomeprazole in bulk and tablet dosage form

Development of analytical method and validation for determination of Lisinopril dihydrate in bulk drug and dosage form using HPTLC method

Development and validation for determination of lisinopril dihydrate in bulk drug and formulation using RP-HPLC method

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