Development and Validation of RP - HPLC Method for Simultaneous Determination of Dicyclomine and Mefanamic Acid

Shrikrishna, Baokar; Mulgund, Sugandha; Ranpise, Nisharani S.

doi:10.18579/jpcrkc/2014/13/1/78402

Cited by 12 publications

(10 citation statements)

References 4 publications

(1 reference statement)

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“…Literature survey revealed that RP-HPLC [7][8][9][10][11][12][13][14][15][16] have been reported for the estimation of vildagliptin and metformin.…”

Section: Vildagliptin [Fig 1] Is Chemically [S]-1-[n-[3-hydroxy-1-admentioning

confidence: 99%

“…The reported methods were not cost effective due to use costly solvents and in some methods the retention time vildagliptin was found to be more [7][8][9][10][11][12][13][14][15][16]. So in the present work a simple, precise, sensitive and stability indicating method was developed by using low-cost solvent acetonitrile with buffer in the ratio of 80:20, detected by using a photodiode array detector which was highly sensitive to detect at a lower concentration.…”

Section: Vildagliptin [Fig 1] Is Chemically [S]-1-[n-[3-hydroxy-1-admentioning

confidence: 99%

“…The retention times in all the reported methods were more when compared to the developed method [10][11][12][13][14]. Hence this method was finalised as an optimized method for the estimation of vildagliptin and metformin.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…The LOD and LOQ were found to be 0.0182 and 0.0553 µg/ml for vildagliptin and 0.4451 and 1.3490 µg/ml for metformin. This showed that the developed method can detect and quantify at lower concentration was highly sensitive whereas other methods is less sensitive [7][8][9][10][11][12][13][14] …”

Section: Limit Of Detection and Limit Of Quantificationmentioning

confidence: 99%

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Stability Indicating Rp-HPLC Method Development and Validation for the Simultaneous Determination of Vildagliptin and Metformin in Pharmaceutical Dosage Form

Jayaprakash¹,

Natesan²

2017

Int J Pharm Pharm Sci

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Objective: The present study was aimed to develop a rapid, accurate, linear, sensitive and validate stability-indicating high performance liquid chromatographic [RP-HPLC] method for determination of vildagliptin and metformin in pharmaceutical dosage form. Methods:The chromatographic separation was performed on kromasil-C18 column [4.5 x 250 mm; 5 µm] using a mobile phase consisting of 0.05 mmol potassium dihydrogen phosphate buffer: acetonitrile [80:20 v/v], [pH adjusted to 3.5 using orthophosphoric acid]. The flow rate is 0.9 ml/min and the detection was carried out at 263 nm. Results:The chromatographic condition, the peak retention time of metformin and vildagliptin were found to be 2.215 min and 2.600 min respectively. Stress testing was performed in accordance with an international conference on harmonization [ICH] Q1A R2 guidelines. The method was validated as per ICH Q2 R1 guidelines. The calibration curve was found to be linear in the concentration range of 5-17.5 µg/ml and 50-175 µg/ml for vildagliptin and metformin. The limit of detection and quantification was found to be 0.0182 µg/ml and 0.0553 µg/ml for vildagliptin and 0.4451 µg/ml and 1.3490 µg/ml for metformin respectively. Conclusion:A new sensitive, simple and stability indicating reverse-phase high-performance liquid chromatography [RP-HPLC] method has been developed and validated for the determination of vildagliptin and metformin. The proposed method can be used for routine determination of vildagliptin and metformin.

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“…Literature survey revealed that RP-HPLC [7][8][9][10][11][12][13][14][15][16] have been reported for the estimation of vildagliptin and metformin.…”

Section: Vildagliptin [Fig 1] Is Chemically [S]-1-[n-[3-hydroxy-1-admentioning

confidence: 99%

Section: Vildagliptin [Fig 1] Is Chemically [S]-1-[n-[3-hydroxy-1-admentioning

confidence: 99%

Section: Chromatographic Conditionsmentioning

confidence: 99%

Section: Limit Of Detection and Limit Of Quantificationmentioning

confidence: 99%

See 2 more Smart Citations

Stability Indicating Rp-HPLC Method Development and Validation for the Simultaneous Determination of Vildagliptin and Metformin in Pharmaceutical Dosage Form

Jayaprakash¹,

Natesan²

2017

Int J Pharm Pharm Sci

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“…[3][4][5][6][7][8][9] A successful study is done for estimation of by. However, the development of simultaneous estimation of Teneligliptin and Metformin in combined dosage form by RP-HPLC method has not yet been reported.…”

mentioning

confidence: 99%

Analytical Method Development and Validation for Simultaneous Estimation of Teneligliptin and Metformin by Using Rp HPLC

Rajaraman¹

2017

WJPR

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Utilizing experimental design and desirability function in optimizing RP-HPLC method for simultaneous determination of some skeletal muscle relaxants and analgesics

Mozayad,

Fouad,

Elkady

2024

Sci Rep

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An experimental design and response surface methodologies using Plackett–Burman and Box-Behnken designs were applied for selecting and optimizing the most appropriate parameters which significantly affect the separation and quantitative estimation of five skeletal muscle relaxants and four analgesic drugs (baclofen, methocarbamol, dantrolene sodium, orphenadrine citrate, cyclobenzaprine hydrochloride, ketoprofen, etoricoxib, ibuprofen, and mefenamic acid) with a relatively short duration of analysis in a single run. For the separation of the nine drugs, an INERTSIL ODS-V3-5 µm C18 column (250 × 4.6 mm I.D.) was used with the optimum mobile phase conditions (45.15 mM ammonium acetate buffer pH 5.56 adjusted with acetic acid, acetonitrile, and methanol in a ratio of 30.5:29.5:40, v/v/v with a flow rate of 1.5 mL/min) and UV-detection at 220 nm. The optimized method was successfully subjected to the validation steps as described in ICH guidelines for linearity, precision, accuracy, robustness, and sensitivity. The optimized and validated method was effectively applied to determine the content of the studied drugs in their pharmaceutical preparations and to expand its applicability to the counterfeit estimation of etoricoxib in different brands of tablet dosage forms.

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Development and Validation of RP - HPLC Method for Simultaneous Determination of Dicyclomine and Mefanamic Acid

Cited by 12 publications

References 4 publications

Stability Indicating Rp-HPLC Method Development and Validation for the Simultaneous Determination of Vildagliptin and Metformin in Pharmaceutical Dosage Form

Stability Indicating Rp-HPLC Method Development and Validation for the Simultaneous Determination of Vildagliptin and Metformin in Pharmaceutical Dosage Form

Analytical Method Development and Validation for Simultaneous Estimation of Teneligliptin and Metformin by Using Rp HPLC

Utilizing experimental design and desirability function in optimizing RP-HPLC method for simultaneous determination of some skeletal muscle relaxants and analgesics

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