Development and Validation of New Spectrophototmetric Methods for the Determination of Sitagliptin

Abstract

Sitagliptin (Figure 1) is an antidiabetic agent which is an oral dipeptidyl peptidase-4 inhibitor which exerts its actions in type 2 diabetes patients by slowing the inactivation of incretin hormones. Sitagliptin (C 16 H 15 F 6 N 5 O; Mol. Wt. 407.314 g/mol) is chemically (R)-4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro [1,2,4] triazolo [4,3a] pyrazin-7(8H)-yl]-1-(2,4,5-Trifluoro phenyl) butan-2 amine. It stimulates insulin secretion when hyperglycemia is present and inhibits glucagon secretion [1-4]. Various spectrophotometric methods [5-19] have been developed for the assay of Sitagliptin in literature and the present methods were compared with the previously published methods. The authors have chosen five different reagents for the assay of Sitagliptin in pharmaceutical dosage forms present study and validated [20].