Development and validation of LC–MS/MS method for quantification of bisphenol A and estrogens in human plasma and seminal fluid

Vítků, Jana; Chlupáčová, Tereza; Sosvorova, Lucie; Hampl, Richard; Hill, Martin; Heráček, Jiří; Bičı́ková, Marie; Stárka, L

doi:10.1016/j.talanta.2015.03.013

Cited by 86 publications

(52 citation statements)

References 38 publications

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“…For the detection of progesterone and testosterone, the protocol for liquid chromatography-tandem mass spectrometry (LC-MS/MS) after Sosvorova et al (2015) was used. However, the protocol for LC-MS/MS following Vitku et al (2015) was applied to measure E 2 . Briefly, the methods consist of plasma extraction with diethyl ether followed by the appropriate derivatization step (to enhance detection responses of steroids in the MS) and separation using the ultra-high performance liquid chromatography Eksigent ultraLC 110 system (Redwood City, CA, USA).…”

Section: Hormone Level Validationmentioning

confidence: 99%

Ovarian control of growth and sexual size dimorphism in a male-larger gecko

Kubička

Schořálková

Červenka

et al. 2016

Journal of Experimental Biology

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Sexual size dimorphism (SSD) reflects sex-specific solutions to the allocation of energy among growth, reproduction and survival; however, the proximate mechanisms behind these solutions are still poorly known even in vertebrates. In squamates, sexual differences in body size used to be attributed to direct energy allocation to energetically demanding processes, largely to reproduction. In addition, SSD is assumed to be controlled by specific endogenous mechanisms regulating growth in a sex-specific manner, namely masculinization by male gonadal androgens or feminization by ovarian hormones. We performed a manipulative growth experiment in females of the male-larger gecko Paroedura picta in order to test the reproductive cost hypothesis, the male androgen hypothesis and the ovarian hormone hypothesis. Specifically, we investigated the effect of total ovariectomy, prepubertal ovariectomy, unilateral ovariectomy, and total ovariectomy followed by exogenous estradiol, dihydrotestosterone or testosterone treatment, on female growth in comparison to males and reproductively active females. The present results and the results of our previous experiments do not support the hypotheses that SSD reflects direct energy allocation to reproduction and that male gonadal androgens are involved. However, all lines of evidence, particularly the comparable growth of reproducing intact and unilaterally ovariectomized females, were concordant with the control of SSD by ovarian hormones. We suggest that feminization of growth by female gonadal hormones should be taken into consideration as an endogenous pathway responsible for the ontogeny of SSD in squamates.

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Section: Hormone Level Validationmentioning

confidence: 99%

Ovarian control of growth and sexual size dimorphism in a male-larger gecko

Kubička

Schořálková

Červenka

et al. 2016

Journal of Experimental Biology

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“…The results are summarized in Table 2. As the matrix was urine, a biological matrix, the bioanalytical validation method from the Food and Drug Administration (FDA) guidelines was used to validate the method because this method is the one most commonly used in cases of biological samples [54,55], particularly for urine [56]. However, it is more restrictive in terms of number of repetition as others guidelines, such as the recommendations provided by the International Conference For the determination of the LOD and LOQ several strategies are described in the literature [43].…”

Section: Validation Of the Methodsmentioning

confidence: 99%

Development of an analytical method for the targeted screening and multi-residue quantification of environmental contaminants in urine by liquid chromatography coupled to high resolution mass spectrometry for evaluation of human exposures

Cortéjade

Kiss

Cren

et al. 2016

Talanta

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“…BPA is frequently quantified by gas chromatography coupled with mass spectrometry (GC/MS) [14][15][16][17][18], highperformance liquid chromatography (HPLC) [19][20][21], and liquid chromatography coupled with mass spectrometry (LC/MS or LC/MS/MS) [16,[22][23][24]. These methods allow high sensitivity and specificity but are labor intensive, expensive, and time consuming because of their complex pretreatment steps.…”

Section: Introductionmentioning

confidence: 99%

Donor/acceptor nanoparticle pair-based singlet oxygen channeling homogenous chemiluminescence immunoassay for quantitative determination of bisphenol A

et al. 2016

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Bisphenol A (BPA) is widely used in consumer products such as plastic bottles and food containers. It has become a ubiquitous environmental contaminant and poses a serious risk to human health. A rapid, sensitive, and highthroughput method for detecting BPA is therefore desirable. Herein, a donor/acceptor nanoparticle pair-based singlet oxygen channeling chemiluminescence homogenous immunoassay is developed for the determination of BPA. The donor nanoparticles were modified with phthalocyanine as a photosensitizer and were then coated with streptavidin. The acceptor nanoparticles were doped with thioxene derivatives and Eu(III) as a chemiluminescence emitter and then coated with anti-BPA antibody. Under light irradiation, oxygen near the donor surface transforms to singlet oxygen ( 1 O 2 ), which migrates to the acceptor and reacts with it, generating luminescence. Because 1 O 2 has a very short lifetime, luminescence is generated only when the donor and acceptor are in close proximity. This occurs when they are brought together by the antigen/antibody and streptavidin/biotin reaction. Based on this singlet oxygen channeling mechanism, a competitive homogenous chemiluminescence immunoassay for BPA was developed on 384 microplates. The assay exhibited linear detection over the range 10-1000 ng/mL and a limit of detection of 2.9 ng/mL. The intra-and inter-assay precisions were both below 5.1 %. The average recoveries of three spiked samples in tap and river water samples were in the range 95.5-121.0 %, in agreement with values obtained using highperformance liquid chromatography. The homogeneous assay is rapid, low cost, sensitive, and allows high-throughput, so is well suited for screening large numbers of environmental samples.

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Development and validation of LC–MS/MS method for quantification of bisphenol A and estrogens in human plasma and seminal fluid

Cited by 86 publications

References 38 publications

Ovarian control of growth and sexual size dimorphism in a male-larger gecko

Ovarian control of growth and sexual size dimorphism in a male-larger gecko

Development of an analytical method for the targeted screening and multi-residue quantification of environmental contaminants in urine by liquid chromatography coupled to high resolution mass spectrometry for evaluation of human exposures

Donor/acceptor nanoparticle pair-based singlet oxygen channeling homogenous chemiluminescence immunoassay for quantitative determination of bisphenol A

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