Development and Validation of HPLC-Dad Method for the Determination of Bisoprolol in Tablet Dosage Forms

Kondratova, Yuliya; Logoyda, Liliya; Voloshko, Yuliia; Megied, Ahmed Abdel; Кorobko, Dmytro; Soroka, Yuriy

doi:10.22159/ijap.2017v9i6.21616

Cited by 25 publications

(20 citation statements)

References 6 publications

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“…In the present study, optimization and critical evaluation of mobile phase composition (gradient), flow rate, and analytical column were important to obtain good resolution of peaks of interest from the endogenous components, which, in turn, affect reproducibility and sensitivity of the method [4][5][6]. The resolution of peaks was best achieved with Discovery C18, 50×2.…”

Section: Resultsmentioning

confidence: 98%

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Logoyda

2018

Asian J Pharm Clin Res

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Objective: A simple, rapid high-performance liquid chromatography-mass spectrometry/mass spectrometry (MS/MS) method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution. . The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.400 ml/min into the MS electrospray ionization chamber. The sample volume was 5 µl.Results: Under these conditions, amlodipine was eluted at 1.61 min. According to the Caco-2 test results, amlodipine appeared to have moderate to high permeability. Recovery value for amlodipine is 52.33%. Caco-2 permeability values for amlodipine are in agreement with BCS Class I classification for these drugs and their known high bioavailability in humans. Conclusion:A new rapid method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for the examination of amlodipine from Caco-2 cell monolayers.

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Section: Resultsmentioning

confidence: 98%

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Logoyda

2018

Asian J Pharm Clin Res

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“…Our objеctivе of the chromatоgraphіc method develоpment wаs to achiеve a peak tailing factor <1.5, retention time in between 1 and 3 min, along with gооd resоlution. [9][10][11] Ascentis express columns, based on Fusеd-core pаrticle technоlogy, prоvide more than twice the speed and efficiency of traditiоnal cоlumns at half the backpressure of sub-2-µm columns. This objеctive was obtainеd using mobile phase consisting of methanol and 0.1% solution of trifluoroacetic acid (40/60, v/v).…”

Section: Discussionmentioning

confidence: 99%

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Logoyda¹

2018

AJP

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Introduction:In recent years, the principle and theory behind solvent extraction has gained wide acceptance as one of the new tools of modern pharmaceutical analysis. Analytical chemists have been using solvent extraction in the laboratory for many years for extracting molecular species from aqueous solutions by organic solvents. However, the requirements and objectives of an analytical procedure are quite different from those of a profit producing mill flow sheet. Depending on the nature, aggregate state, sample concentration and method of analysis, and various sampling procedures are used: Moisture removal, grinding, decomposition, dissolution, melting, elution, removal of the matrix, dilution, concentration, etc. The objective of this research was to select the optimal conditions for the extraction for captopril by organic solvents from water solutions in dependence on pH solutions. Materials and Methods: The chromatographic analysis of captopril performed on liquid chromatography ACQUITY Arc system. Results: The obtained data testify that the optimal solvents for the extraction of captopril in the process of separating it from objects of biological origin are chloroform and methylene chloride. The amount of substance released by chloroform is 91.48% (at pH 1), methylene chloride is 97.97% (at pH 1), and hexane is 69.65% (at pH 2). Conclusion: The extraction of captopril by organic solvents from water solutions in dependence on pH solutions has been conducted. As a result of studies, we have found that the optimal extragent is methylene chloride, which is extracted at pH 1-97.97% and chloroform is extracted at pH 1-91.48%.

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“…Therefore, all the experiments were carried out on a C18 column (Zorbax Eclipse ХDB-C18, 5 µm, 2.1 mm × 150 mm) by trying various mobile phase conditions systematically [4][5][6][7][8][9]. After the initial experiments, the optimum conditions were found to be the mobile phase of water:acetonitrile:trifluoroacetic acid (55:45:0.1 v/v/v) mixture pumped at 0.4 mL/min flow rate and 265 nm ultraviolet detection wavelength.…”

Section: Methods Optimizationmentioning

confidence: 99%

Development of High-Performance Liquid Chromatography Method for the Simultaneous Analysis of Amlodipine and Valsartan in Combined Dosage Form and in Vitro Dissolution Studied

Yuryeva¹,

Kondratova²,

Logoyda

2018

Asian J Pharm Clin Res

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Objective: A simple, rapid, and reproducible high-performance liquid chromatography method was developed for the simultaneous determination of amlodipine and valsartan in their combined dosage forms and for drug dissolution studies.Methods: A C 18 column (Zorbax Eclipse ХDB-C18, 5 µm, 2.1 mm × 150 mm) and a mobile phase of water:acetonitrile:trifluoroacetic acid (55:45:0.1 v/v/v) mixture were used for separation and quantification. Analyses were run at a flow rate of 0.4 mL/min and at ambient temperature. The injection volume was 5 µL and the ultraviolet detector was set at 265 nm. The method was validated as per ICH guidelines.Results: Under these conditions, amlodipine and valsartan were eluted at 1.64 min and 4.08 min, respectively. Total run time was shorter than 7 min. The results were 99.6 ± 0.6 and 98.5 ± 0.8 for amlodipine and valsartan, respectively. Valsartan was released within 15 min (98.32%) and amlodipine was also released within 30 min (96.16%) both at a pH of 6.8. Conclusion:The developed method was applied successfully for quality control assay of amlodipine and valsartan in their combination drug product and in vitro dissolution studies.

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Development and Validation of HPLC-Dad Method for the Determination of Bisoprolol in Tablet Dosage Forms

Cited by 25 publications

References 6 publications

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

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Development of High-Performance Liquid Chromatography Method for the Simultaneous Analysis of Amlodipine and Valsartan in Combined Dosage Form and in Vitro Dissolution Studied

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