Development and validation of hplc method for determination of andrographolide in raw material and tablet of ethyl acetate fractions of Andrographis paniculata

Determination of Quercetin in Extracts and Herbal Products of Phyllanthus niruri by TLC Densitometry Method

“…Phyllanthus niruri herb extract must contains a total flavonoid not less than 3.20% as quercetin (Indonesian Herbal Pharmacopoeia, 2017). Analytical marker compound was compound in plant that dominant in plant, easy analysis or have important activities (Ihsan et al, 2020). Some previous analytical method to analysis quercetin were spectrophotometry UV-Vis (Soylak et al, 2020), TLC (Ihsan et al, 2019), HPLC (Dinakaran et al, 2018), LC-MS (Kumar et al, 2015), HPTLC (Amir et al, 2013).…”

Validation of High-Performance Liquid Chromatography (HPLC) Method for Quantification of Ethyl p-Methoxycinnamate in Kaempferia galanga Extract

“…Several parameters for standardizing herbal medicines refer to World Health Organization (WHO) guidelines, including botanical parameters (macroscopic and microscopic), physicochemical (chromatographic fingerprint, loss on drying, ash value, acid insoluble ash, extractive value, moisture content, volatile matter), pharmacological (bitterness value, hemolytic property, astringent property, swelling index, foaming index), and toxicological parameters (arsenic and heavy metals, pesticide residues, microbial contamination) 10,11 . The chromatographic fingerprint is one of the important parameters in the standardization of herbal medicines, which is a method for identifying and determining the content of marker compounds in each plant or herbal medicine to ensure quality and pharmacological activity 12,13 . Marker compounds are compounds or groups of compounds present in plants with or without pharmacological activity 14 .…”