Development and validation of HILIC-UHPLC-ELSD methods for determination of sugar alcohols stereoisomers and its application for bioconversion processes of crude glycerin

Costa, P. P. K. G.; Mendes, T. D.; Salum, T. F. C.; Pacheco, T. F.; Braga, Samira C.; Almeida, J. R. M. de; Gonçalves, Sílvia Belém; Damaso, M. C. T.; Rodrigues, Clenilson Martins

doi:10.1016/j.chroma.2018.12.044

Cited by 10 publications

(5 citation statements)

References 31 publications

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“…The equations of each regression curve and coefficients of determination (R 2 ) are shown in Table 3 . A quadratic regression model was found to be more applicable than a linear regression model for GE species by showing better coefficients of determination, consistent with the known fact that the ELSD response was not linear [ 23 , 26 ]. The coefficients of determination were >0.9999 for all GE species, attesting that the method was reliable for GE quantitation ( Table 3 ).…”

Section: Resultssupporting

confidence: 66%

First Determination of Glycidyl Ester Species in Edible Oils by Reverse-Phase Ultra-Performance Liquid Chromatography Coupled with an Evaporative Light-Scattering Detector

Chen

et al. 2021

Molecules

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In this work, a new ultra-performance liquid chromatograph-evaporative light-scattering detector (UPLC-ELSD) method for quantitation of glycidyl esters (GE) contents in edible oils is presented. The method features complete separation of five GE species within 20 min by a C18 column and gradient elution with a mobile phase consisting of 85% and 2.5% methanol aqueous solutions. The coefficients of regression (R2) were all ≥0.9999 for the linear-quadratic regression curves of GE species in a concentration range of 5~80 μg/mL. The intraday and interday recoveries (%) of GE species in solvent were in a range of 81.3~107.3%, and the intraday and interday coefficients of variation (CVs, %) were all ≤8.6%. The average recovery (%) of GE species spiked in extra-virgin olive oil samples ranged from 88.3~107.8% and the intermediate precision (CV, %) of ≤14% indicated acceptable accuracy and precision. The method exhibited limit of quantification (LOQ) for each GE species (0.6 μg glycidol equivalents/g oil). The method was applied to determine GE concentrations of six commercial oil samples, and total glycidol equivalents were consistent with data obtained by GC-MS method. This UPLC-ELSD method could be adopted for precursory screening and research purposes to improve food safety when MS detectors are unavailable.

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Section: Resultssupporting

confidence: 66%

First Determination of Glycidyl Ester Species in Edible Oils by Reverse-Phase Ultra-Performance Liquid Chromatography Coupled with an Evaporative Light-Scattering Detector

Chen

et al. 2021

Molecules

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“…Primary MR analyses were undertaken separately using single nucleotide polymorphisms (SNPs) associated with erythritol (P < 5 × 10 À8 ) in two of the largest GWAS studies in populations of European ancestry (NIHR and METSIM) (Table 1), which, based on linkage disequilibrium (LD), were partially overlapping (Supplementary Table 3) (13,14). Erythritol and threitol are isomers and cannot be distinguished by mass spectrometry unless separated by gas chromatography or liquid chromatography, with the latter dependent on the type of column used (15)(16)(17)(18). In the METSIM and NIHR GWAS cohorts, liquid chromatography with hydrophilic interaction liquid chromatography column was undertaken to separate erythritol, but GWAS of threitol was not performed (14,15,19).…”

Section: Cohortsmentioning

confidence: 99%

“…Erythritol and threitol are isomers and cannot be distinguished by mass spectrometry unless separated by gas chromatography or liquid chromatography, with the latter dependent on the type of column used (15)(16)(17)(18). In the METSIM and NIHR GWAS cohorts, liquid chromatography with hydrophilic interaction liquid chromatography column was undertaken to separate erythritol, but GWAS of threitol was not performed (14,15,19). We therefore undertook additional MR analyses using instruments from Twins UK/KORA in which erythritol and threitol were separated by gas chromatography prior to measurement with subsequent GWAS for both (18).…”

Section: Cohortsmentioning

confidence: 99%

Erythritol as a Potential Causal Contributor to Cardiometabolic Disease: A Mendelian Randomization Study

Khafagy,

Paterson,

Dash

2023

Diabetes

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People with type 2 diabetes frequently use low-calorie sweeteners to manage glycemia and reduce caloric intake. Use of erythritol, a low-calorie sweetener, has increased recently. Higher circulating concentration associates with major cardiac events and metabolic disease in observational data, prompting some concern. As observational data may be prone to confounding and reverse causality, we undertook bidirectional Mendelian randomization (MR) to investigate potential causal associations between erythritol and coronary artery disease (CAD), body mass index (BMI), waist-hip-ratio (WHR), glycemic and renal traits in cohorts of European ancestry. Analyses were undertaken using instruments comprising genome-wide-significant variants from three cohorts with erythritol measurement. Across instruments, we did not find supportive evidence that increased erythritol increases CAD (b=-0.033±0.02, p=0.14; b=0.46±0.37, p=0.23). MR indicates erythritol may decrease BMI (b=-0.04±0.018, p=0.03; b=-0.04±0.0085, p=1.23x10-5; b=-0.083±0.092, p=0.036) with potential evidence from one instrument of increased BMI adjusted for waist-hip ratio (b=0.046±0.022, p=0.035). No evidence of causal association was found with other traits. In conclusion, we did not find supportive evidence from MR that erythritol increases cardiometabolic disease. These findings await confirmation in well-designed prospective studies.

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“…Both amine and amide as well as traditional C18 stationary phases have been used to analyze carbohydrates and in particular simple sugars [18][19][20][21]. Therefore, an investigation into the analyses of these compounds on the silica hydride-based amide phase provides a useful insight for determining if this column can perform satisfactorily in this important application.…”

Section: Applications Of Silica Hydride Amide Stationary Phasementioning

confidence: 99%

Chromatographic characterization of a silica hydride‐based amide stationary phase

Pesek

Matyska

Tardiff

et al. 2021

J of Separation Science

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An amide phase based on a porous silica hydride support material is tested for retention characteristics in both the reversed-phase and aqueous normal-phase modes. A series of retention maps (capacity factor vs. mobile phase composition) were obtained using reference standards of varying analyte sizes, functionalities, and polarities. An assessment of the specific column selectivity is made and classes of compounds are identified that show high potential for effective retention, resolution, and efficiency when using amide functionalized silica hydride columns for reversed-phase and aqueous-normal phase separation. Several practical applications are presented that illustrate the capabilities of this particular column format.

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Development and validation of HILIC-UHPLC-ELSD methods for determination of sugar alcohols stereoisomers and its application for bioconversion processes of crude glycerin

Cited by 10 publications

References 31 publications

First Determination of Glycidyl Ester Species in Edible Oils by Reverse-Phase Ultra-Performance Liquid Chromatography Coupled with an Evaporative Light-Scattering Detector

First Determination of Glycidyl Ester Species in Edible Oils by Reverse-Phase Ultra-Performance Liquid Chromatography Coupled with an Evaporative Light-Scattering Detector

Erythritol as a Potential Causal Contributor to Cardiometabolic Disease: A Mendelian Randomization Study

Chromatographic characterization of a silica hydride‐based amide stationary phase

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