Development and Validation of High-Performance Liquid Chromatography Method for Determination of Atorvastatin in Polymeric Nanocapsules

Viçozzi, Gabriel Pedroso; Neto, Luiz Torres; Silva, Pedro Santos da; Nenê, Luiza Rodrigues; Colomé, Letícia Marques; Bender, Eduardo André

doi:10.34117/bjdv6n8-697

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…The content of NCAtG and NCAtU was realized with formulations made within 48 h. Therefore, the formulations were previously treated with acetonitrile, with promoted the dissolution of the polymer and subsequent release of the active from the inside of the nanostructure. The determination of ATV in the formulations was performed by HPLC using previously described methodology [25].…”

Section: Content Of Formulationsmentioning

confidence: 99%

Physico-chemical Characterization and Biosafety Evaluation of Atorvastatin Nanocapsules Co-encapsulated with Ginger Oil or Grape Seed Oil

Vicozzi,

Neto,

De Oliveira Pereira

et al. 2023

BioNanoSci.

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Cardiovascular diseases are one of the major causes of deaths around the world. The leading cause is related to high cholesterol levels, therefore, controlling these levels has been a successful strategy. Among the drugs used for this purpose, atorvastatin (ATV) has great e cacy, however some side effects reduce patient adhesion. In this context, the development of ATV polymeric nanocapsules coencapsulated with ginger (NCAtG) or grape seed (NCAtU) oil can reduce ATV toxicity and increase its e cacy. The objectives of this work were to evaluate the safety and e cacy of this nanoformulations in different experimental models. The formulations had nanometric sizes and suitable physio chemical parameters. The biosafety was evaluated in terms of hemoglobin measurement, liberation of erythrocyte LDH and blood coagulation cascade by the extrinsic (PTT) and intrinsic (aPTT) pathways after exposed to the nanoformulations, were just high concentrations caused alterations in these parameters. Also, there is no cytotoxicity in the 3T3 cell lines and no alterations in the comet assay. The in vivo assays in C. elegans showed no alterations, in the survival, brood size and length. And nally, the formulations demonstrated signi cative effect about the reduction of the lipidic levels of the nematodes, with high lipid levels.

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Section: Content Of Formulationsmentioning

confidence: 99%

Physico-chemical Characterization and Biosafety Evaluation of Atorvastatin Nanocapsules Co-encapsulated with Ginger Oil or Grape Seed Oil

Vicozzi,

Neto,

De Oliveira Pereira

et al. 2023

BioNanoSci.

Self Cite

View full text Add to dashboard Cite

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Physico-chemical characterization and biosafety evaluation of atorvastatin nanocapsules co-encapsulated with ginger oil or grape seed oil

Viçozzi

Neto

Pereira

et al. 2023

Preprint

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View full text Add to dashboard Cite

Cardiovascular diseases are one of the major causes of deaths around the world. The leading cause is related to high cholesterol levels, therefore, controlling these levels has been a successful strategy. Among the drugs used for this purpose, atorvastatin (ATV) has great efficacy, however some side effects reduce patient adhesion. In this context, the development of ATV polymeric nanocapsules co-encapsulated with ginger (NCAtG) or grape seed (NCAtU) oil can reduce ATV toxicity and increase its efficacy. The objectives of this work were to evaluate the safety and efficacy of this nanoformulations in different experimental models. The formulations had nanometric sizes and suitable physio chemical parameters. The biosafety was evaluated in terms of hemoglobin measurement, liberation of erythrocyte LDH and blood coagulation cascade by the extrinsic (PTT) and intrinsic (aPTT) pathways after exposed to the nanoformulations, were just high concentrations caused alterations in these parameters. Also, there is no cytotoxicity in the 3T3 cell lines and no alterations in the comet assay. The in vivo assays in C. elegans showed no alterations, in the survival, brood size and length. And finally, the formulations demonstrated significative effect about the reduction of the lipidic levels of the nematodes, with high lipid levels.

show abstract

Development and Validation of High-Performance Liquid Chromatography Method for Determination of Atorvastatin in Polymeric Nanocapsules

Abstract: Development and validation of high-performance liquid chromatography method for determination of atorvastatin in polymeric nanocapsulesDesenvolvimento e validação de método de cromatografia líquida de alta eficiência para determinação de atorvastatina em nanocápsulas poliméricas

Cited by 2 publications

References 11 publications

Physico-chemical Characterization and Biosafety Evaluation of Atorvastatin Nanocapsules Co-encapsulated with Ginger Oil or Grape Seed Oil

Physico-chemical Characterization and Biosafety Evaluation of Atorvastatin Nanocapsules Co-encapsulated with Ginger Oil or Grape Seed Oil

Physico-chemical characterization and biosafety evaluation of atorvastatin nanocapsules co-encapsulated with ginger oil or grape seed oil

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