Development and Validation of High-Content Analysis for Screening HDAC6-Selective Inhibitors

Nong, Yunhong; Hou, Yingyong; Pu, Yuting; Li, Si; Yan, Lei

doi:10.1177/24725552211002463

Cited by 1 publication

(1 citation statement)

References 42 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multiple methods could be used to screen for inhibitors, including virtual screening by molecular docking and molecular dynamics simulation, in vitro enzymatic assays, and cell-based cytoblot assay and high-content analysis. [83,84] With the specific inhibitors, validation of the therapeutic effect of targeting NDST3 can be performed on established models that recapitulate the clinical disease-relevant symptoms of LSDs.…”

Section: Neurodegenerative Lysosomal Storage Disordersmentioning

confidence: 99%

Tubulin deacetylase NDST3 modulates lysosomal acidification: Implications in neurological diseases

Tang

Wang

2022

BioEssays

View full text Add to dashboard Cite

Neurological diseases (NDs), featured by progressive dysfunctions of the nervous system, have become a growing burden for the aging populations. N‐Deacetylase and N‐sulfotransferase 3 (NDST3) is known to catalyze deacetylation and N‐sulfation on disaccharide substrates. Recently, NDST3 is identified as a novel deacetylase for tubulin, and its newly recognized role in modulating microtubule acetylation and lysosomal acidification provides fresh insights into ND therapeutic approaches using NDST3 as a target. Microtubule acetylation and lysosomal acidification have been reported to be critical for activities in neurons, implying that the regulators of these two biological processes, such as the previously known microtubule deacetylases, histone deacetylase 6 (HDAC6) and sirtuin 2 (SIRT2), could play important roles in various NDs. Aberrant NDST3 expression or tubulin acetylation has been observed in an increasing number of NDs, including amyotrophic lateral sclerosis and frontotemporal dementia (ALS/FTD), schizophrenia and bipolar disorder, Alzheimer's disease (AD), and Parkinson's disease (PD), suggesting that NDST3 is a key player in the pathogenesis of NDs and may serve as a target for development of new treatment of NDs.

show abstract

Section: Neurodegenerative Lysosomal Storage Disordersmentioning

confidence: 99%