Development and validation of fluorescence spectroscopic assays to evaluate antioxidant efficacy. Application to metal chelators

Arora, Arti; Strasburg, Gale M.

doi:10.1007/s11746-997-0021-4

Cited by 48 publications

(37 citation statements)

References 44 publications

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“…Greater changes were observed for callistephin chloride. They, however, disappeared on addition of cholesterol to the DPPC membrane; cholesterol imposes order on the hydrophilic phase of the membrane, and in the presence of anthocyanins the order increases slightly [28,29,36]. For the Prodan probe a high intensity of polarity is recorded in an aqueous medium at 512 nm.…”

Section: Discussionmentioning

confidence: 97%

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Bonarska-Kujawa

Pruchnik

Kleszczyńska

2012

Cellular and Molecular Biology Letters

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Anthocyanins are one of the main flavonoid groups. They are responsible for, e.g., the color of plants and have antioxidant features and a wide spectrum of medical activity. The subject of the study was the following compounds that belong to the anthocyanins and which can be found, e.g., in strawberries and chokeberries: callistephin chloride (pelargonidin-3-O-glucoside chloride) and ideain chloride (cyanidin-3-O-galactoside chloride). The aim of the study was to determine the compounds' antioxidant activity towards the erythrocyte membrane and changes incurred by the tested anthocyanins in the lipid phase of the erythrocyte membrane, in liposomes composed of erythrocyte lipids and in DPPC, DPPC/cholesterol and egg lecithin liposomes. In particular, we studied the effect of the two selected anthocyanins on red blood cell morphology, on packing order in the lipid hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC and DPPC/cholesterol liposomes. Fluorimetry with the Laurdan and Prodan probes indicated increased packing density in the hydrophilic phase of the membrane in the presence of anthocyanins. Using the fluorescence probes DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified. The compounds slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The study has shown that both anthocyanins are incorporated into the outer region of the erythrocyte membrane, affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The investigation proved that the compounds penetrate only the outer part of the external lipid layer of liposomes composed of erythrocyte lipids, DPPC, DPPC/cholesterol and egg lecithin lipids, changing its packing order. Fluorimetry studies with DPH-PA proved that the tested anthocyanins are very effective antioxidants. The antioxidant activity of the compounds was comparable with the activity of Trolox ® .

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Section: Discussionmentioning

confidence: 97%

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Bonarska-Kujawa

Pruchnik

Kleszczyńska

2012

Cellular and Molecular Biology Letters

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“…The mixture was incubated in the dark at a room temperature for 16 h. The ABTS Extent of membrane lipid oxidation by fl uorimetric method The antioxidant activity of the extracts was determined by using the fl uorimetric method, based on the relation between fl uorescence intensity of the DPH-PA probe and concentration of free radicals in the solution. Fluorescence intensity decreases with an increasing concentration of free radicals [ Arora & Strasburg, 1997]. To a phosphate buffer (131 mmol/L NaCl, 1.79 mmol/L KCl, 0.86 mmol/L MgCl 2 , 11.79 mmol/L Na 2 HPO 4 ×2H 2 O, 1.80 mmol/L Na 2 H 2 PO 4 ×H 2 O, pH 7.4) containing erythrocyte ghosts and the fl uorescence probe were added proper amounts (0.001, 0.005, 0.0075, 0.01, 0.05, 0.01 mg/mL) of the extracts.…”

Section: Assay Of Antioxidant Capacity Of Extractsmentioning

confidence: 99%

Antioxidant Activity of Extracts from Apple, Chokeberry and Strawberry.

Bonarska-Kujawa

Sarapuk²,

Bielecki³

et al. 2012

Pol. J. Food Nutr. Sci.

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The aim of the study was to determine the protective action of extracts from apple, strawberry and chokeberry with respect to linoleic acid and the biological membrane exposed to oxidation induced by physicochemical factors. The activity of the extracts was determined by measuring inhibition of lipid oxidation in red blood cell membrane, induced with UVC radiation and the AAPH radical. The protective effect of the extracts was essayed fl uorimetrically and spectrophotometrically. These results together with the ones obtained earlier explain the mechanism of the interaction between the extracts and the red blood cell membrane. The mechanism consists in the incorporation into the membrane and screening the cell against oxidation. The results indicate that the extracts possess very good antioxidant properties, since at the highest concentrations used (0.1 mg/mL) they protect the biological membranes almost entirely against oxidation. Among the extracts studied the best antioxidant properties were exhibited by the apple fruit, which gave 80% or 100% protection of the membrane at 0.05 mg/mL concentration of dry matter, for UVC and AAPH inductors, respectively.

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“…The probe's fluorescence decreased with its rising oxidation caused by free radicals, supplied by AAPH at a final concentration of 1 M at 37 °C. As a measure of the degree of erythrocyte and lipid membranes oxidation was assumed the value of relative intensity of DPH-PA fluorescence [20]. It was calculated as a ratio of fluorescence intensity after 30 min of oxidation in the presence of antioxidants to the initial value of the intensity.…”

Section: Antioxidant Activity -Fluorometric Methodsmentioning

confidence: 99%

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

Strugała¹,

Cyboran-Mikołajczyk²,

Wyspiańska³

et al. 2017

Rec. Nat. Prod.

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Abstract:The study is concerned with biological activity of quince (Q) fruit extract (Cydonia oblonga Mill.) towards the phosphatidylcholine liposome and the natural lipid-protein erythrocyte membrane, as well as will explaining the40 way of interaction of the extract with the membrane. The results showed that Q extract protects lipids against oxidation induced by AAPH compound to a similar extent in two models of membrane. Studies with probes (Laurdan and DPH) located at different depths within the membrane lipid bilayer showed that extract caused an increase of the packing order of the polar heads of lipids and a slight decrease in mobility of the acyl chains. Such results suggest that extract molecules associate with the lipid and lipid-protein membrane and can stop the propagation of free radicals within the bilayer by modifying the membrane fluidity. Furthermore, Q extract resulted in the inhibition of the activity of enzymes (cyclooxygenase-1 and cyclooxygenase-2) probably involved in inflammatory reactions in the body. The experimental results proved that extract components can bind to the main plasma protein -human serum albumin, and the quenching mechanism was suggested as static. The obtained quince-albumin binding constants show that the extract probably can be transported from the circulatory system to reach its target organ.

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Development and validation of fluorescence spectroscopic assays to evaluate antioxidant efficacy. Application to metal chelators

Cited by 48 publications

References 44 publications

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Antioxidant Activity of Extracts from Apple, Chokeberry and Strawberry.

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

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