Development and Validation of Entacapone in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry

Panigrahy, Uttam Prasad; Sahoo, Nalini Kanta; Reddy, A. Sunil Kumar

doi:10.14233/ajchem.2015.19323

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The literature survey revealed that various methods were reported for the estimation of ETC; including spectrophotometry [5][6], spectrofluorimetry [7], liquid chromatography-tandem mass spectrometry (LC-ESI-MS/ MS) [8][9], Liquid Chromatography with electrochemical detection [10] or UV detection [11] and reversed-phase liquid chromatography with amperometric detection [12].…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Poly‐o‐phenylenediamine Electrochemical Sensor for Entacapone

Radi

Abd-Ellatief

2021

Electroanalysis

View full text Add to dashboard Cite

We have developed a molecularly imprinted polymer (MIP) electrochemical sensor for entacapone (ETC) based on an electropolymerised polyphenylenediamine (Po‐PD) on a glassy carbon electrode (GCE) surface. The direct electropolymerisation of the o‐phenylenediamine monomer (o‐PD) was carried out with ETC as a template. The steps of electropolymerization process, template removal and binding of the analyte were tested by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) using [Fe(CN)6]3−/[Fe(CN)6]4 − as a redox probe. The operation of the sensor has been investigated by differential pulse voltammetry (DPV). Under optimal experimental conditions, the response of the DPV was linearly proportional to the ETC concentration between 1.0×10−7 and 5.0×10−6 M ETC with a limit of detection (LOD) of 5.0×10−8 M. The developed sensor had excellent selectivity without detectable cross‐reactivity for levodopa and carbidopa. The MIP sensor was successfully used to detect ETC in spiked human serum samples.

show abstract