Development and validation of dual-cardiotoxicity evaluation method based on analysis of field potential and contractile force of human iPSC-derived cardiomyocytes / multielectrode assay platform

PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development

“…Considering that hHO is a mixed population of several cells, such as endothelial cells and cardiomyocytes 57 , it remained uncertain whether the impact of sPIBF on hHO is due to its direct effect on cardiomyocyte differentiation or indirect effect resulting from enhanced endothelial angiogenesis. To address this issue, we conducted a two-dimensional culture of human iPSC-derived cardiomyocytes 58 , which revealed the direct impact of PIBF1 on cardiomyocyte differentiation (Fig. 6c ).…”

Development of heart organoid cryopreservation method through Fe3O4 nanoparticles based nanowarming system

“…Among FP parameters, spike amplitude (SA) indicates an increase in the propagating action potential in the QRS portion where depolarization occurs (Figure 4C). [ 9 ] Before cryopreservation, a waveform showing regular coupling of depolarization/repolarization could be observed at the site where electrodes of the MEA plate were in contact (Figure 4D,E). As a result of MEA comparison between groups after thawing for 7 days, no waveform was observed in the control, whereas regular FP with weaker SA than the waveform of pre‐experiment appeared in the Fe 3 O 4 group (Figure 4E).…”

“…Human induced pluripotent stem cells (hiPSCs) were manufactured from human foreskin fibroblasts (BJ-fibroblasts; ATCC; VA, USA) via reprogramming using episomal vectors. [8,9] hiPSCs were cultured in mTeSR (STEMCELL Technologies Inc; Vancouver, BC, Canada) medium and subcultured in Matrigel (Corning Inc.; Corning, NY, USA)-coated cell culture dishes (Eppendorf; Hamburg, Germany) at a 1:3 ratio when cells reached 90% confluency for 3 days. For generation of hiPSCderived heart organoids (hHOs), detached hiPSCs were collected in a conical tube using mTeSR medium containing 10 μM Y-27632 (Tocris Bioscience, Bristol, UK) and 10% Matrigel.…”

“…In order to minimize the error range of the electrical signal, it was maintained for 10 min in an environmental condition similar to the incubator, and then field potential-based electrophysiological signals were measured for 3 min using AxIS software. [9,11]…”

iPSC-Derived Cardiomyocytes in Inherited Cardiac Arrhythmias: Pathomechanistic Discovery and Drug Development

“…To confirm these findings, these drugs were tested over several laboratories/facilities, commercial cardiomyocyte types and different MEA platforms and reproducible concentration-dependent electrophysiological responses were reported, indicating that iPSC-CMs can predict clinical QT prolongation and/or arrhythmogenic potential of drug compounds [69][70][71]. Lee at al showed that addition of a contractility assay (impedance measurement) into the evaluation of cardiotoxicity provides/allows more mechanistic insights on the drug effect [72]. As discussed above, 3D heart-on-a-chip models are also being tested, holding promise for even better prediction of cardiotoxic and pro-arrhythmic drug effects as they better recapitulated the clinical effects compared to 2D iPSC-CM models as they present occasionally with arrhythmias that are not reported in adult cardiomyocytes [73,74].…”