Development and validation of automated methods for COVID-19 PCR MasterMix preparation
Giorgio Fedele,
Graham Hill,
Amelia Sweetford
et al.
Abstract:Polymerase Chain Reaction (PCR)-based assays were widely deployed during the SARS-CoV-2 pandemic for population-scale testing. High-throughput molecular diagnostic labora-tories required a high degree of process automation to cope with huge testing demand, fast turn-around times and quality requirements. However, the critical step of preparing a PCR MasterMix has often been neglected by process developers and optimisers, and is largely dependent upon operator skill for the manual pipetting of reagents to const… Show more
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