Development and Validation of Aptasensor Based on MnO2 for the Detection of Sulfadiazine Residues

Abstract

The monitoring of sulfadiazine (SDZ) is of great significance for food safety, environmental protection, and human health. In this study, a fluorescent aptasensor based on MnO2 and FAM-labeled SDZ aptamer (FAM-SDZ30-1) was developed for the sensitive and selective detection of SDZ in food and environmental samples. MnO2 nanosheets adsorbed rapidly to the aptamer through its electrostatic interaction with the base, providing the basis for an ultrasensitive SDZ detection. Molecular dynamics was used to explain the combination of SMZ1S and SMZ. This fluorescent aptasensor exhibited high sensitivity and selectivity with a limit of detection of 3.25 ng/mL and a linear range of 5–40 ng/mL. The recoveries ranged from 87.19% to 109.26% and the coefficients of variation ranged from 3.13% to 13.14%. In addition, the results of the aptasensor showed an excellent correlation with high-performance liquid chromatography (HPLC). Therefore, this aptasensor based on MnO2 is a potentially useful methodology for highly sensitive and selective detection of SDZ in foods and environments.

Multi-modal nanoprobe-enabled biosensing platforms: a critical review

“…Compared with other sensors of the same type, it has a wider detection range and higher reliability. 152 The detection limits were 28 ng mL −1 and 2 ng mL −1 for colorimetric and FL methods, respectively. The recoveries were 88.28–108.44% and 90.27–106.04%, respectively.…”

Recent Progress in Biosensors Based on Biorecognition Molecules

“…They believe that after integration with low-cost photodetectors, ECL biosensors will have great potential in commercial applications [ 5 ]. Meanwhile, several works focus on contaminant determination in food and environments, like sulfadiazine (SDZ) [ 6 ], deoxynivalenol (DON) [ 7 ] and dibutyl phthalate (DBP) [ 8 ]. With regard to the detection systems, antibodies or aptamers were used to recognize the targets, which contributed to good selectivity.…”