Development and validation of an LC-MS/MS screening method for macrolide and quinolone residues in baby food

Grutes, Jônatas Vieira; Ferreira, Rosana Gomes; Pereira, Mararlene Ulberg; Candido, Felipe Stanislau; Spisso, Bernardete Ferraz

doi:10.1080/03601234.2021.1872324

Cited by 8 publications

(5 citation statements)

References 20 publications

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“…The use of LC-MS/MS based on a triple quadrupole MS is common in the analysis of veterinary drugs that are present in complex matrices, although this requires the use of high-resolution instruments (Grutes et al 2021). Thus, in the current study, we employed the positive/negative ionization modes in combination with the ESI MRM mode.…”

Section: Optimization Of the Lc-ms/ms Conditionsmentioning

confidence: 99%

Validated LC-MS/MS method for the simultaneous analysis of veterinary drugs in cultured meat media

Gu¹,

Kim²,

Lee³

et al. 2022

Preprint

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The development of alternative meat, such as cultured meat, is of current interest due to its potential to solve various problems common to the livestock industry. Cultured meat is defined as in vitro, lab-grown, or synthetic meat, and is produced through the differentiation and culture of animal cells. To increase the efficiency of this culture veterinary drugs are commonly employed. It is therefore necessary to develop a method that allows the amounts of veterinary drugs, growth-promoting hormones, and antibiotics to be determined in cultured meat, to ultimately ensure an adequate safety profile. In this study, we report the development of an analytical method based on liquid chromatography–tandem mass spectrometry for the simultaneous analysis of various veterinary drugs. Validation of the developed method indicated a high accuracy and precision, in addition to a linearity of 0.9868–0.9999. Using this method, the veterinary drugs present in samples of culture media were analyzed. Importantly, penicillin was detected in the range of 2 to 44,259 mg/L, and no other veterinary drugs were detected. It is therefore expected the implementation of this method will improve the safety profile of the cultured meat market, in addition to securing the safety of new food technologies.

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Section: Optimization Of the Lc-ms/ms Conditionsmentioning

confidence: 99%

Validated LC-MS/MS method for the simultaneous analysis of veterinary drugs in cultured meat media

Gu¹,

Kim²,

Lee³

et al. 2022

Preprint

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“…There are some published LC-MS/MS methods for the determination of spiramycin in different matrices. [12][13][14][15] However, they have not considered the possibility of solvent intrusion into spiramycin chemical structure which may be a source of error in the quantication of spiramycin. The current study was carried out based on the structural features of spiramycin.…”

Section: Introductionmentioning

confidence: 99%

Challenges for the determination of spiramycin in aqueous matrices using LC-MS/MS: evidence for the solvent intrusion on the molecule integrity

et al. 2022

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“…Multiple technologies have been applied to detect residues of T&T in food, such as high-performance liquid chromatography (HPLC), micellar electrokinetic capillary chromatography (MECC), high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and liquid chromatography-tandem mass high-resolution spectrometry (LC-HRMS) [12][13][14][15][16][17]. However, sophisticated and expensive equipment, professional personnel, and complex preprocessing methods are needed for the above methods [17,18], and existing research methods are somewhat limited in that they cannot detect multiclass residues [19]; importantly, they are not convenient for on-site diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Wang

Yang

et al. 2021

Foods

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Tylosin and tilmicosin (T&T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T&T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T&T was 1.87~7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T&T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 °C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection.

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Development and validation of an LC-MS/MS screening method for macrolide and quinolone residues in baby food

Cited by 8 publications

References 20 publications

Validated LC-MS/MS method for the simultaneous analysis of veterinary drugs in cultured meat media

Validated LC-MS/MS method for the simultaneous analysis of veterinary drugs in cultured meat media

Challenges for the determination of spiramycin in aqueous matrices using LC-MS/MS: evidence for the solvent intrusion on the molecule integrity

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

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