Development and validation of an isotope dilution ultra‐high pressure liquid chromatography‐tandem mass spectrometry method for quantitation of serum 25‐hydroxyvitamin D3, D2 and 3‐epi‐D3
Abstract:Our aim was to develop and validate a method for separate quantitation of 25‐hydroxyvitamin D2 (25OHD2) and 25‐hydroxyvitamin D3 (25OHD3) and its epimer (epi‐25OHD3), with complete chromatographic resolution of 25OHD3 and epi‐25OHD3. Serum (100 uL) was diluted with internal standards (d3‐25OHD2 and d6‐25OHD3) and extracted with hexanes. The organic layer was evaporated, reconstituted and injected onto a Kinetex PFP column (2.1 × 100 mm × 1.7 um) using an Accela UHPLC system coupled with a triple quadrupole Van… Show more
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.