Development and Validation of an NMR-Based Identity Assay for Bacterial Polysaccharides

Abeygunawardana, Chitrananda; Williams, Thomas; Sumner, James S.; Hennessey, John P.

doi:10.1006/abio.1999.4470

Cited by 89 publications

(43 citation statements)

References 34 publications

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“…3. The NMR spectrum of native 20␣ PS is closely related to the spectrum of reference serotype 20 PS included in PPV-23 (18). Moreover, the spectrum of dOAc 20␣ PS is identical to that of dOAc reference serotype 20 PS (data not shown).…”

Section: The Ps Capsules Of the Serotype 20 Subtypes Differ In The Ammentioning

confidence: 77%

Biochemical, Genetic, and Serological Characterization of Two Capsule Subtypes among Streptococcus pneumoniae Serotype 20 Strains

Calix

Porambo

Brady

et al. 2012

Journal of Biological Chemistry

134

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Section: The Ps Capsules Of the Serotype 20 Subtypes Differ In The Ammentioning

confidence: 77%

Biochemical, Genetic, and Serological Characterization of Two Capsule Subtypes among Streptococcus pneumoniae Serotype 20 Strains

Calix

Porambo

Brady

et al. 2012

Journal of Biological Chemistry

134

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“…All spectra were acquired at a regulated temperature of 298 K and calibrated to the residual water peak (4.766 ppm). For the interpretation of the received carbon and hydrogen shifts, results from previous studies were used as guidance for the capsule structure determination of the 19A capsule extracts (4,39,40).…”

Section: Methodsmentioning

confidence: 99%

Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment

et al. 2016

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Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13). Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported. Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules. We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy. After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred. Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, Nacetyl-mannosamine, and glucose. In summary, our study suggests that no structural variance dependent of the nutritional environment or the subtype exists. The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule structure variant. However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.

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“…Is true that many of these methods have revolutionized identity testing from the technological point of view, they have the disadvantages of being very expensive, not being available to all laboratories, they need a highly qualified staff and in most of the cases require prior treatment of the sample. [8,9,10] Immunoassays are based on the Antigen-Antibody reaction. In immunoagglutination techniques, this Ag-Ac reaction becomes visible (agglutination) because one of the two molecules are forming part of or artificially joined to particulate systems such as the surface of red blood cells, platelets, leukocytes, or latex particles, etc.…”

Section: Identity Test Of Quimi-vio Vaccinementioning

confidence: 99%

“…Immunoagglutination technique specifically using latex reagents is within immunoassays and have been very useful because they are the fastest and can be carried out anywhere without the need of can technology complexity. [9,10,11] In the case of Quimi-Vio identity test, highly specific techniques would be needed to identify each PsC (of the seven present) in the multivalent formulation.…”

Section: Introductionmentioning

confidence: 99%

Immunoagglutination Test Using Monoclonal Antibodies Coupled to Latex Particles to Identify The Streptococcus Pneumoniae Capsular Polysaccharides Serotype 1, 5, 6B, 14 And 19F In Quimi-Vio Vaccine

Aznar¹

2017

IJVR

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Development and Validation of an NMR-Based Identity Assay for Bacterial Polysaccharides

Cited by 89 publications

References 34 publications

Biochemical, Genetic, and Serological Characterization of Two Capsule Subtypes among Streptococcus pneumoniae Serotype 20 Strains

Biochemical, Genetic, and Serological Characterization of Two Capsule Subtypes among Streptococcus pneumoniae Serotype 20 Strains

Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment

Immunoagglutination Test Using Monoclonal Antibodies Coupled to Latex Particles to Identify The Streptococcus Pneumoniae Capsular Polysaccharides Serotype 1, 5, 6B, 14 And 19F In Quimi-Vio Vaccine

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