Development and validation of an HPLC method for the determination of verapamil residues in supports of cleaning procedure

Milenović, Dragan M.; Milošević, Snežana P.; Đurić, Svetlana Lj.; Nasković, Daniela Č.; Mitić, Snežana S.

doi:10.1134/s1061934813060051

Cited by 6 publications

(2 citation statements)

References 9 publications

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“…The Brazilian pharmacopoeia recommends that the quality control of VP is done by ultraviolet absorption spectrophotometry . In the literature are also found several methods for determination of VP in formulations and in biological fluids such as high performance liquid chromatography . Besides, there are methods for VP analysis based on UV‐Visible absorption spectroscopy , potentiometry , capillary electrophoresis and electroanalytical methods .…”

Section: Introductionmentioning

confidence: 99%

Selective Determination of Verapamil in Pharmaceutics and Urine Using a Boron‐doped Diamond Electrode Coupled to Flow Injection Analysis with Multiple‐pulse Amperometric Detection

et al. 2018

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This work presents a simple and low‐cost method for fast and selective determination of Verapamil (VP) in tablets and human urine samples using a boron‐doped diamond working electrode (BDD) coupled to a flow injection analysis system with multiple pulse amperometric detection (FIA‐MPA). The electrochemical behaviour of VP in 0.1 mol L−1 sulfuric acid showed three merged oxidation peaks at around +1.4 V and upon reverse scan, one reduction peak at 0.0 V (vs. Ag/AgCl). The MPA detection was performed applying a sequence of three potential pulses on BDD electrode: (1) at +1.6 V for VP oxidation, (2) at +0.2 V for reduction of the oxidized product and (3) at +0.1 V for cleaning of the working electrode surface. The FIA system was optimized with injection volume of 150 μL and flow rate of 3.5 mL min−1. The method showed a linear range from 0.8 to 40.0 μmol L−1 (R>0.99) with a low limit of detection of 0.16 μmol L−1, good repeatability (RSD<2.2 %; n=10) and sample throughput (45 h−1). Selective determination of VP in urine was performed at+0.2 V due to absence of interference from ascorbic and uric acids in this potential. The addition‐recovery tests in both samples were close to 100 % and the results were similar to an official method.

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Section: Introductionmentioning

confidence: 99%

Selective Determination of Verapamil in Pharmaceutics and Urine Using a Boron‐doped Diamond Electrode Coupled to Flow Injection Analysis with Multiple‐pulse Amperometric Detection

et al. 2018

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“…The drug is used clinically for the treatment of various cardiovascular diseases such as supraventricular tachyarrhythmias, hypertension, nephrotic syndrome, variant angina, cardiomyopathy and all types of ischemic diseases of the heart and vessels [ 1 , 3 ]. To control the quality of verapamil HCl in pharmaceutical preparations, a variety of techniques have been used to analyze it including high performance liquid chromatography [ 4 , 5 ], mass fragmentography [ 6 ], spectrophotometry [ 7 ], capillary electrophoresis [ 8 ], fluorescence [ 9 , 10 ], gas chromatography with mass spectrometry [ 11 ], etc. Among these methods, the fluorescence technique presented high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Determination of Verapamil HCl in Pharmaceutical Preparations by a Fluorescent Nano Probe Based on CdTe/CdS/ZnS Quantum Dots

Sohail

Wei

et al. 2017

Nanomaterials

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An analytical technique based on fluorescence quenching of CdTe/CdS/ZnS quantum dots (QDs) was developed to quantify verapamil in commercially available preparations. Various reaction parameters were optimized and the method developed was validated. One way analysis of variance (ANOVA) and post hoc tests at a 5% significance level were performed to justify the significance of the variation in observations. The linear range of the verapamil concentration was 0.25–5 µg/mL while the limit of detection was 20 µg/mL. Recovery and relative standard deviations were not more than ±10% of the actual amount and <5.9%, respectively. Foreign materials, common metal ions and pharmaceutical excipients of dosage forms caused little interference. To verify the application of the analytical method, the quantity of verapamil in commercially available dosage forms was measured. Verapamil content in the tablets and injections was not more than ±10% of the stated amount and it conformed to the specifications of both the British and the United States pharmacopoeias. In the case of statistical analysis, p-value was <0.05 in almost all levels of all parameters except for the optimized level of system. It can be concluded from the results that the designed method is simple, reliable, cost effective, selective, rapid and sensitive enough to be used for quantitative measurement of the verapamil HCl in dosage forms for quality control purposes.

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Detection and quantification of residues and metabolites of medicinal products in environmental compartments, food commodities and workplaces. A review

Bottoni

Caroli²

2015

Journal of Pharmaceutical and Biomedical Analysis

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Development and validation of an HPLC method for the determination of verapamil residues in supports of cleaning procedure

Cited by 6 publications

References 9 publications

Selective Determination of Verapamil in Pharmaceutics and Urine Using a Boron‐doped Diamond Electrode Coupled to Flow Injection Analysis with Multiple‐pulse Amperometric Detection

Selective Determination of Verapamil in Pharmaceutics and Urine Using a Boron‐doped Diamond Electrode Coupled to Flow Injection Analysis with Multiple‐pulse Amperometric Detection

Determination of Verapamil HCl in Pharmaceutical Preparations by a Fluorescent Nano Probe Based on CdTe/CdS/ZnS Quantum Dots

Detection and quantification of residues and metabolites of medicinal products in environmental compartments, food commodities and workplaces. A review

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