Development and validation of an UHPLC-HRMS protocol for the analysis of flavan-3-ol metabolites and catabolites in urine, plasma and feces of rats fed a red wine proanthocyanidin extract

Pereira-Caro, Gema; Ordóñez-Díaz, José Luis; Ludwig, Iziar A.; Gaillet, Sylvie; Mena, Pedro; Rio, Daniele Del; Rouanet, Jean-Max; Bindon, Keren; Moreno-Rojas, José Manuel; Crozier, Alan

doi:10.1016/j.foodchem.2018.01.083

Cited by 30 publications

(37 citation statements)

References 37 publications

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“…The metabolic behaviors of proanthocyanidins can be divided into two parts: the gastric and intestinal part. The acidic environment in gastric digestion may possibly lead to degradation of oligomeric and polymeric proanthocyanidins, while the gut microbiota might convert proanthocyanidins into more than 30 kinds of metabolites and further derivatives, including various kinds of phenolic acids and valerolactones (Pereira‐Caro et al., ).…”

Section: Metabolic Pathways Of Proanthocyanidins During Digestionmentioning

confidence: 99%

“…Most of the ingested proanthocyanidins can reach the colon for further fermentation by gut microbiota; thus, more and more studies have focused on the mutual effects between proanthocyanidins and gut microbiota, including how proanthocyanidins change the composition of bacteria and what metabolites are produced from their interactions (Figure ). More than 30 kinds of metabolites and further derivatives, including various kinds of phenolic acids and valerolactones, are reported to be the metabolites of proanthocyanidins by gut microbiota, and some of these metabolites have been proved to possess health‐promoting activities (Pereira‐Caro et al., ). Particularly, some phenolic acids and phenylvalerolactones produced from proanthocyanidins by gut microbiota are reported to exhibit greater biological activities than their parent compounds (Monagas et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Rethinking the Mechanism of the Health Benefits of Proanthocyanidins: Absorption, Metabolism, and Interaction with Gut Microbiota

Tao

Zhang

Shen

et al. 2019

Comp Rev Food Sci Food Safe

101

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Proanthocyanidins, as the oligomers or polymers of flavan‐3‐ol, are widely discovered in plants such as fruits, vegetables, cereals, nuts, and leaves, presenting a major part of dietary polyphenols. Although proanthocyanidins exert several types of bioactivities, such as antioxidant, antimicrobial, cardioprotective, and neuroprotective activity, their exact mechanisms remain unclear. Due to the complexity of the structure of proanthocyanidins, such as their various monomers, different linkages and isomers, investigation of their bioavailability and metabolism is limited, which further hinders the explanation of their bioactivities. Since the large molecular weight and degree of polymerization limit the bioavailability of proanthocyanidins, the major effective site of proanthocyanidins is proposed to be in the gut. Many studies have revealed the effects of proanthocyanidins from different sources on changing the composition of gut microbiota based on in vitro and in vivo models and the bioactivities of their metabolites. However, the metabolic routes of proanthocyanidins by gut microbiota and their mutual interactions are still sparse. Thus, this review summarizes the chemistry, absorption, and metabolic pathways of proanthocyanidins ranging from monomers to polymers, as well as the mutual interactions between proanthocyanidins and gut microbiota, in order to better understand how proanthocyanidins exert their health‐promoting functions.

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Section: Metabolic Pathways Of Proanthocyanidins During Digestionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rethinking the Mechanism of the Health Benefits of Proanthocyanidins: Absorption, Metabolism, and Interaction with Gut Microbiota

Tao

Zhang

Shen

et al. 2019

Comp Rev Food Sci Food Safe

101

View full text Add to dashboard Cite

show abstract

“…This section focuses on discussing the aspects of col these samples in relation to bioavailability and metabolism studies. Urine samples are of special interest, since the excreted metabolites may be a tion of the metabolic transformations produced in the original compounds of the e Regarding this type of sample, there are studies that use only a single interval of collection [59,60] and studies that use multiple collection intervals [58,61]. In g urine samples are usually collected after establishing several time intervals for bette itoring of metabolite excretion.…”

Section: Biological Samplesmentioning

confidence: 99%

“…In the case of urine in human models, it is mostly co at the following intervals: (0-2), (2-5), (5)(6)(7)(8) and (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24) h. In animal assays, they are u Urine samples are of special interest, since the excreted metabolites may be a reflection of the metabolic transformations produced in the original compounds of the extract. Regarding this type of sample, there are studies that use only a single interval of sample collection [59,60] and studies that use multiple collection intervals [58,61]. In general, urine samples are usually collected after establishing several time intervals for better monitoring of metabolite excretion.…”

Section: Biological Samplesmentioning

confidence: 99%

Recent Analytical Approaches for the Study of Bioavailability and Metabolism of Bioactive Phenolic Compounds

et al. 2022

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The study of the bioavailability of bioactive compounds is a fundamental step for the development of applications based on them, such as nutraceuticals, functional foods or cosmeceuticals. It is well-known that these compounds can undergo metabolic reactions before reaching therapeutic targets, which may also affect their bioactivity and possible applications. All recent studies that have focused on bioavailability and metabolism of phenolic and terpenoid compounds have been developed because of the advances in analytical chemistry and metabolomics approaches. The purpose of this review is to show the role of analytical chemistry and metabolomics in this field of knowledge. In this context, the different steps of the analytical chemistry workflow (design study, sample treatment, analytical techniques and data processing) applied in bioavailability and metabolism in vivo studies are detailed, as well as the most relevant results obtained from them.

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“…Another newly developed method is Q Exactive hybrid quadrupole-orbitrap mass spectrometry (Q Exactive MS), which combines high-performance quadrupole precursor selection with high-resolution and accurate-mass orbitrap detection. In the field of PC analysis, from 2015 until the present, this hybrid technology has been used in only a few studies, to identify proanthocyanidin [50], procyanidins [59], resveratrol [106], flavanone, and secoiridoids [29]. Most (80%) of these studies used heated electrospray ionization (HESI), which generally gives improved signals compared to the more traditional unheated ESI (Table 2).…”

Section: Nontargeted Approachesmentioning

confidence: 99%

Metabolomics Technologies for the Identification and Quantification of Dietary Phenolic Compound Metabolites: An Overview

et al. 2021

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In the search for natural products with properties that may protect against or slow down chronic and degenerative diseases (e.g., cancer, and cardiovascular and neurodegenerative conditions), phenolic compounds (PC) with benefits for human health have been identified. The biological effects of PC in vivo depend on their bioavailability, intestinal absorption, metabolism, and interaction with target tissues. The identification of phenolic compounds metabolites (PCM), in biological samples, after food ingestion rich in PC is a first step to understand the overall effect on human health. However, their wide range of physicochemical properties, levels of abundance, and lack of reference standards, renders its identification and quantification a challenging task for existing analytical platforms. The most frequent approaches to metabolomics analysis combine mass spectrometry and NMR, parallel technologies that provide an overview of the metabolome and high-power compound elucidation. In this scenario, the aim of this review is to summarize the pre-analytical separation processes for plasma and urine samples and the technologies applied in quantitative and qualitative analysis of PCM. Additionally, a comparison of targeted and non-targeted approaches is presented, not available in previous reviews, which may be useful for future metabolomics studies of PCM.

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Development and validation of an UHPLC-HRMS protocol for the analysis of flavan-3-ol metabolites and catabolites in urine, plasma and feces of rats fed a red wine proanthocyanidin extract

Cited by 30 publications

References 37 publications

Rethinking the Mechanism of the Health Benefits of Proanthocyanidins: Absorption, Metabolism, and Interaction with Gut Microbiota

Rethinking the Mechanism of the Health Benefits of Proanthocyanidins: Absorption, Metabolism, and Interaction with Gut Microbiota

Recent Analytical Approaches for the Study of Bioavailability and Metabolism of Bioactive Phenolic Compounds

Metabolomics Technologies for the Identification and Quantification of Dietary Phenolic Compound Metabolites: An Overview

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