Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: Application to a comparative pharmacokinetic study of aqueous extracts of raw and salt-processed Achyranthes bidentata

Tao, Yi; Du, Yingshan; Li, Weidong; Cai, Baochang

doi:10.1016/j.jpba.2017.12.024

Cited by 19 publications

(8 citation statements)

References 14 publications

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“…The values of T max of the five saponins were within 1.0 h after oral administration. A double‐peak phenomenon of the five saponins is presented which was in agreement with our previous pharmacokinetic study (Tao, Du, Li, & Cai, ). The double‐peak phenomenon of chikusetsusaponin IV, chikusetsusaponin IVa and ginsenoside Ro was reported in the literature (Qi et al, ; Wang, Liu, Guo, & Wang, ; Zheng et al, ), and is probably due to distribution, reabsorption and enterohepatic circulation.…”

Section: Resultssupporting

confidence: 91%

A liquid chromatography–tandem mass spectrometry approach for study the tissue distributions of five components of crude and salt‐processed Radix Achyranthes in rats

Tao

Yan

Cai

2019

Biomedical Chromatography

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This study developed a robust and reliable approach using liquid chromatographytandem mass spectrometry for the simultaneous determination of five saponins in rat tissues: β-ecdysterone, chikusetsusaponin IV, ginsenoside Ro, 25S-inokosterone and chikusetsusaponin IVa. This is the first report on a comparative tissue distribution study of crude and salt-processed Radix Achyranthes in rats. After one-step protein precipitation by acetonitrile, the tissue samples were sent to LC-MS/MS for multiple reaction monitoring. The retention times of the five saponins and internal standard were 1.77, 3.14, 3.01, 1.83, 3.26 and 4.77 min. The standard curves showed good linear regression (r 2 > 0.9991) in the range of 10.3-1562.5 ng/mL. The intra-and inter-day accuracy and precision were within 15% of the nominal concentration.The recoveries of the five saponins were 92.0-99.9%. Finally, this approach was successfully applied to tissue distribution analysis of the five saponins after oral administration of crude and salt-processed Radix Achyranthes in rats. The largest concentration of the five saponins was observed in kidney after salt-processing, which indicated that processing could enhance the bioavailability.

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Section: Resultssupporting

confidence: 91%

A liquid chromatography–tandem mass spectrometry approach for study the tissue distributions of five components of crude and salt‐processed Radix Achyranthes in rats

Tao

Yan

Cai

2019

Biomedical Chromatography

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“…A Thermo Scientific Dionex Ultimate 3000 UHPLC system with a Thermo High-Resolution Q Exactive Focus mass spectrometer (Thermo, Germany) were used for analysis, and the analytical method was derived from previous studies. , The injection volume was 1.0 μL. The chromatographic column was presented on a 100 mm × 2.1 mm Hypersil Gold instrument (Thermo Fisher Scientific) with a particle size of 1.9 μm.…”

Section: Methodsmentioning

confidence: 99%

Oleanane-Type Saponins Biosynthesis in Panax notoginseng via Transformation of β-Amyrin Synthase Gene from Panax japonicus

Zhang

Jiang

et al. 2019

J. Agric. Food Chem.

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Oleanane-type saponins considered as the main medicinal ingredients in Panax japonicus are not found in Panax notoginseng. β-Amyrin synthase (βAS) was recognized as the first key enzyme in the biosynthetic branch of oleanane-type saponins. In this study, βAS gene from P. japonicus (PjβAS) was transferred into P. notoginseng cells. Along with PjβAS expression in the transgenic cells, the expression levels of several key enzyme genes related to triterpenoid saponins biosynthesis and the content of P. notoginseng saponins were also increased. Two oleanane-type saponins, chikusetsusaponin IV and chikusetsusaponin IVa, contained in P. japonicus were first discovered in transgenic P. notoginseng cells. This study successfully constructed a biosynthetic pathway of oleanane-type saponins in P. notoginseng by introducing just one gene into the species. On the basis of this discovery and previous studies, the common biosynthetic pathway of triterpenoid saponins in Panax genus may be unified to some extent.

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“…TIC chromatograms of the individual standard compounds were shown in Figure 1. The eight components were unambiguously assigned as β-ecdysterone, 25S-inokosterone [15], achyranthoside D [16], ginsenoside Ro [15], chikusetsusaponin IV [17], achyranthoside C [16], chikusetsaponin IVa [17] and 13-HODE [8].…”

Section: Optimization Of Chromatographic and Mass Spectrometric Condimentioning

confidence: 99%

Integrated response surface methodology and UHPLC coupled with triple quadrupole time‐of‐flight MS quantitation to investigate the salt‐processing chemistry of traditional Chinese medicines: A case study on Achyranthes bidentata

Tao

Jia

et al. 2018

Separation Science Plus

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A sensitive ultra high performance liquid chromatography with tandem mass spectrometry approach for the quantification of eight bioactive constituents, including β‐ecdysterone, 25S‐inokosterone, achyranthoside D, ginsenoside Ro, chikusetsusaponin IV, achyranthoside C, chikusetsaponin IVa, and 13‐hydroxyoctadecadienoic acid, in raw and salt‐processed Achyranthes bidentata was developed and validated. Response surface methodology was employed for optimization of three parameters of ultrasound‐assisted extraction process, including the extraction time, methanol percentage, and solvent volume, to give optimum extraction conditions as follows: extraction time of 40.2 min, methanol percentage 67.2% v/v, and solvent volume of 48.4 mL. The established approach was interrogated in terms of linearity, sensitivity, precision, repeatability as well as recovery. Intra‐ and inter‐assay variability for all analytes ranged from 1.6 to 3.1% and from 2.0 to 3.2%, respectively. The standard addition method determined recovery rates for each analyte (99.2–103.6%). In addition, the developed approach was applied to 20 batches of collected raw samples of Achyranthes bidentata and their salt‐processed products. After salt‐processing, the contents of β‐ecdysterone, 25S‐inokosterone, achyranthoside D, chikusetsusaponin IV and achyranthoside C were elevated, while the content of ginsenoside Ro was decreased. The results showed that experimental design in combination with ultra high performance liquid chromatography with tandem mass spectrometry quantification provided insights into the chemistry alteration between raw Achyranthes bidentata and its salt‐processed product.

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Development and validation of an UHPLC–MS/MS approach for simultaneous quantification of five bioactive saponins in rat plasma: Application to a comparative pharmacokinetic study of aqueous extracts of raw and salt-processed Achyranthes bidentata

Cited by 19 publications

References 14 publications

A liquid chromatography–tandem mass spectrometry approach for study the tissue distributions of five components of crude and salt‐processed Radix Achyranthes in rats

A liquid chromatography–tandem mass spectrometry approach for study the tissue distributions of five components of crude and salt‐processed Radix Achyranthes in rats

Oleanane-Type Saponins Biosynthesis in Panax notoginseng via Transformation of β-Amyrin Synthase Gene from Panax japonicus

Integrated response surface methodology and UHPLC coupled with triple quadrupole time‐of‐flight MS quantitation to investigate the salt‐processing chemistry of traditional Chinese medicines: A case study on Achyranthes bidentata

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