Development and validation of an on-line multidimensional SPE-LC–MS/MS method for the quantitation of Tetrandrine in blood samples

Çağlar, Sena; Morello, Rosa; Boos, Karl-Siegfried

doi:10.1016/j.jchromb.2015.02.021

Cited by 11 publications

(4 citation statements)

References 33 publications

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“…FL detection is more selective and sensitive as compared to UV and may be as effective as mass spectrometric methods in biological fluid samples containing very low amounts of drug ( 73 , 74 ). Although the instrument is relatively sophisticated and expensive, mass spectrometric detection provides a high-throughput analysis of large sample batches even without preconcentration with repeatable results since most of the drug substances do not have enough double bonds or functional groups to exhibit FL ( 75 , 76 ). Solvent consumption could be reduced by downscaling conventional PP, LLE and SPE ( 57 ).…”

Section: Discussionmentioning

confidence: 99%

Liquid Chromatographic Methods for COVID-19 Drugs, Hydroxychloroquine and Chloroquine

Bilgin

Evcil

Yazgi

et al. 2020

Journal of Chromatographic Science

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COVID-19 has been a threat throughout the world since December 2019. In attempts to discover an urgent treatment regime for COVID-19, hydroxychloroquine (HCQ) and chloroquine (CQ) have been on solidarity clinical trial. However, many countries have pulled HCQ and CQ from their COVID-19 treatment regimens recently, some countries still continue using them for patients who have previously started HCQ and CQ and they may complete their course under the supervision of a doctor. HCQ and CQ are 4-aminoquinoline drugs and it is safe to use them for autoimmune diseases, rheumatoid arthritis, systemic lupus erythematosus and malaria as well. Determination of CQ, HCQ and their metabolites in biologic fluids and in pharmaceuticals has great importance, especially for pharmacokinetics, pharmacodynamics and epidemiological studies. In this review, liquid chromatographic methods developed in the last 10 years were summarized focusing on sample preparation and detection methods for HCQ and CQ determination in biological fluids and pharmaceutical preparations. It is hoped that this article could be helpful to facilitate the use of these drugs in clinical trials or drug research studies as it provides comprehensive information on the reported analytical methods.

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Section: Discussionmentioning

confidence: 99%

Liquid Chromatographic Methods for COVID-19 Drugs, Hydroxychloroquine and Chloroquine

Bilgin

Evcil

Yazgi

et al. 2020

Journal of Chromatographic Science

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“…SPE methods coupled to LC has been widely applied to analyze a wide range of compounds present in biological matrices [76][77][78][79][80][81]. As an example, Caglar et al [82] developed a multidimensional-SPE method for on-line clean-up and subsequent LC-MS/MS analysis of Tetrandrine, a bisbenzylisoquinoline alkaloid isolated from the dried root of Stephania tetrandra S. Moore, in blood samples. According to this methodology, for the first LC dimension a LiChrospher R ADS RP 4 RAM SPE-column (20 mm × 2 mm, 25 m) was used to obtain size-exclusion of the large molecules while a second LC dimension with an OASIS R MCX SPE-column (10 mm × 2 mm) provided a size-selective fractionation.…”

Section: Applications Utilizing the Switching Valve Approachmentioning

confidence: 99%

On‐line approaches for the determination of residues and contaminants in complex samples

Fumes

Andrade

Franco

et al. 2016

J of Separation Science

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The determination of residues and contaminants in complex matrices such as in the case of food, environmental, and biological samples requires a combination of several steps to succeed in the aimed goal. At least three independent steps are integrated to provide the best available situation to deal with such matrices: (1) a sample preparation technique is employed to isolate the target compounds from the rest of the matrix; (2) a chromatographic (second) step further "purifies" the isolated compounds from the co-extracted matrix interferences; (3) a spectroscopy-based device acts as chromatographic detector (ideally containing a tandem high-resolution mass analyzer) for the qualitative and quantitative analysis. These techniques can be operated in different modes including the off-line and the on-line modes. The present report focus the on-line coupling techniques aiming the determination of analytes present in complex matrices. The fundamentals of these approaches as well as the most common set ups are presented and discussed, as well as a review on the recent applications of these two approaches to the fields of bioanalytical, environmental, and food analysis are critically discussed.

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“…In attempt to study the pharmacokinetics and tissue distribution of DNR and Tet in rats, a sensitive and reliable liquid chromatography with tandem mass spectrometry method (LC-MS/MS) was developed and validated for the quantification of DNR and Tet in rat plasma or tissues. [13][14][15][16]…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and Tissue Distribution of PLGAPLL- PEG-TF Nanoparticles Loaded with Daunorubicin and Tetrandrine Following Intravenous Injection in the Rats Using LC-MS/MS

Wang¹,

Yang²,

Liu³

et al. 2018

IJPER

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Objective: A sensitive, rapid and reliable LC-MS/MS method was developed for simultaneously determination of daunorubicin (DNR) and tetrandrine (Tet) in rat plasma and tissues. Methods: The pharmacokinetics and tissue distribution of DNR/Tet-PLGA-PLL-PEG-NPs-Tf (D/T-PPP-NPs-Tf), DNR/Tet-PLGA-PLL-PEG-NPs (D/T-PPP-NPs) and DNR/Tet (D/T) solution were compared following intravenous injection in the rats. Result: Compare to D/T solution, the parameters of DNR and Tet in D/T-PPP-NPs-Tf group presented significantly higher AUC (p<0.01) and lower V (p<0.05). Compared with D/T solution, the distribution of DNR in groups of D/T-PPP-NPs and D/T-PPP-NPs-Tf were higher in liver (p<0.01), lung (p<0.05), fat (p<0.05) and testicle (p<0.05), and were lower in heart, spleen, kidney (p<0.01), brain and intestine (p<0.01). The distribution of Tet in D/T-PPP-NPs and D/T-PPP-NPs-Tf groups were higher in liver, brain and uterus (p<0.05), and were lower in heart, spleen, lung, kidney and fat (p<0.05) compared with D/T solution. Conclusion: The LC-MS/MS method was specific, sensitive, rapid and reliable for the simultaneously determination of DNR and Tet in rat plasma and tissues. Compared with D/T solution, D/T-PPP-NPs-Tf tended to stay in the blood after injection. The distribution of D/T-PPP-NPs and D/T-PPP-NPs-Tf were significantly changed.

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Development and validation of an on-line multidimensional SPE-LC–MS/MS method for the quantitation of Tetrandrine in blood samples

Cited by 11 publications

References 33 publications

Liquid Chromatographic Methods for COVID-19 Drugs, Hydroxychloroquine and Chloroquine

Liquid Chromatographic Methods for COVID-19 Drugs, Hydroxychloroquine and Chloroquine

On‐line approaches for the determination of residues and contaminants in complex samples

Pharmacokinetics and Tissue Distribution of PLGAPLL- PEG-TF Nanoparticles Loaded with Daunorubicin and Tetrandrine Following Intravenous Injection in the Rats Using LC-MS/MS

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