Development and validation of an HPLC method for the determination of seven penicillin antibiotics in veterinary drugs and bovine blood plasma

Samanidou, Victoria F.; Giannakis, Dimitrios; Papadaki, Antonia

doi:10.1002/jssc.200800758

Cited by 23 publications

(11 citation statements)

References 15 publications

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“…There are a few studies regarding metabolite quantification for samples in which the matrix is a sponge 13. This type of validation is more common in samples of different biological origins 14, 15, in the food industry 16 and even in plant metabolites 17. In this particular case, there is no information available regarding a methodology for the treatment of sponge samples.…”

Section: Resultsmentioning

confidence: 99%

Validation and evaluation of an HPLC methodology for the quantification of the potent antimitotic compound (+)‐discodermolide in the Caribbean marine sponge Discodermia dissoluta

Valderrama

Castellanos

Zea³

2010

J of Separation Science

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The sponge Discodermia dissoluta is the source of the potent antimitotic compound (+)-discodermolide. The relatively abundant and shallow populations of this sponge in Santa Marta, Colombia, allow for studies to evaluate the natural and biotechnological supply options of (+)-discodermolide. In this work, an RP-HPLC-UV methodology for the quantification of (+)-discodermolide from sponge samples was tested and validated. Our protocol for extracting this compound from the sponge included lyophilization, exhaustive methanol extraction, partitioning using water and dichloromethane, purification of the organic fraction in RP-18 cartridges and then finally retrieving the (+)-discodermolide in the methanol-water (80:20 v/v) fraction. This fraction was injected into an HPLC system with an Xterra RP-18 column and a detection wavelength of 235 nm. The calibration curve was linear, making it possible to calculate the LODs and quantification in these experiments. The intra-day and inter-day precision showed relative standard deviations lower than 5%. The accuracy, determined as the percentage recovery, was 99.4%. Nine samples of the sponge from the Bahamas, Bonaire, Curaçao and Santa Marta had concentrations of (+)-discodermolide ranging from 5.3 to 29.3 microg/g(-1) of wet sponge. This methodology is quick and simple, allowing for the quantification in sponges from natural environments, in situ cultures or dissociated cells.

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Section: Resultsmentioning

confidence: 99%

Validation and evaluation of an HPLC methodology for the quantification of the potent antimitotic compound (+)‐discodermolide in the Caribbean marine sponge Discodermia dissoluta

Valderrama

Castellanos

Zea³

2010

J of Separation Science

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“…In the literature, there are many analytical methods for the individual determination of amoxicillin trihydrate or enrofloxacin [4,5,6,7,8,9,10,11,12,13,14], but not for both of them simultaneously, by high-performance liquid chromatography (HPLC)-UV. The objective of this study was therefore to develop and validate a simple reverse-phase (RP)-HPLC method using a UV-PDA (photodiode array) detector to simultaneously quantify amoxicillin and enrofloxacin.…”

Section: Introductionmentioning

confidence: 99%

A Validated Stability-Indicating HPLC Method for Simultaneous Determination of Amoxicillin and Enrofloxacin Combination in an Injectable Suspension

Batrawi¹,

Wahdan²,

Al‐Rimawi

2017

Sci. Pharm.

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The combination of amoxicillin and enrofloxacin is a well-known mixture of veterinary drugs; it is used for the treatment of Gram-positive and Gram-negative bacteria. In the scientific literature, there is no high-performance liquid chromatography (HPLC)-UV method for the simultaneous determination of this combination. The objective of this work is to develop and validate an HPLC method for the determination of this combination. In this regard, a new, simple and efficient reversed-phase HPLC method for simultaneous qualitative and quantitative determination of amoxicillin and enrofloxacin, in an injectable preparation with a mixture of inactive excipients, has been developed and validated. The HPLC separation method was performed using a reversed-phase (RP)-C18e (250 mm × 4.0 mm, 5 μm) column at room temperature, with a gradient mobile phase of acetonitrile and phosphate buffer containing methanol at pH 5.0, a flow rate of 0.8 mL/min and ultraviolet detection at 267 nm. This method was validated in accordance with the Food and Drug Administration (FDA) and the International Conference on Harmonisation (ICH) guidelines and showed excellent linearity, accuracy, precision, specificity, robustness, ruggedness, and system suitability results within the acceptance criteria. A stability-indicating study was also carried out and indicated that this method can also be used for purity and degradation evaluation of these formulations.

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“…The basic structure of penicillin is a thiazolidine ring attached to a β-lactam ring. Five members of this class of antibiotics are determined in the present study: Oxacillin, methicillin, nafcillin, dicloxacillin and cloxacillin (Figure 1) [1][2].…”

Section: Introductionmentioning

confidence: 99%

Identification of Penicillinase-Resistant Penicillins in Human Urine by RPLC Method

Üstün¹,

Demıralay²,

Nane³

2016

Eurasian J Anal Chem

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In this study, the combined effect of the organic modifier content and mobile phase pH has been used to predict the chromatographic behavior of methicillin, oxacillin, nafcillin, cloxacilin and dicloxacillin. The optimum separation condition of these drugs in acetonitrilewater binary mixture was determined from the mobile phase pH dependence of their retention factor. A Synergy Fusion-RP C18 analytical column (150 mm x 4.6 mm I.D., 4 µm) was preferred to carry out the developed method. Chromatographic measurements were done at 30• C with an eluent flow rate of 1 mL min -1 . In order to validate the optimized conditions, these drugs were applied successfully in spiked samples of human urine. Valsartan was chosen as an internal standard. The described method was linear over a concentration range of 2-32 µg mL -1 and 3-48 µg mL -1 for the assay of methicillin, nafcillin, dicloxacillin and oxacillin, cloxacillin respectively. Good linear relationships over the investigated concentration ranges were observed with values of correlation coefficient higher than 0.999 for all of the drugs. The intra-day and inter-day precisions of this method were evaluated with RSD (%) values less than 0,561 and 1,622%, respectively. Optimized assay method was validated according to United States Pharmacopeia (USP) guidelines to confirm linearity, accuracy and precision.

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Development and validation of an HPLC method for the determination of seven penicillin antibiotics in veterinary drugs and bovine blood plasma

Cited by 23 publications

References 15 publications

Validation and evaluation of an HPLC methodology for the quantification of the potent antimitotic compound (+)‐discodermolide in the Caribbean marine sponge Discodermia dissoluta

Validation and evaluation of an HPLC methodology for the quantification of the potent antimitotic compound (+)‐discodermolide in the Caribbean marine sponge Discodermia dissoluta

A Validated Stability-Indicating HPLC Method for Simultaneous Determination of Amoxicillin and Enrofloxacin Combination in an Injectable Suspension

Identification of Penicillinase-Resistant Penicillins in Human Urine by RPLC Method

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