Research-validated clicker questions comprise an easy-to-implement instructional tool that can scaffold student learning while formatively assessing students' knowledge. We present findings from the development, validation and implementation, in consecutive years, of a Clicker Question Sequence (CQS) on measurement uncertainty as it applies to two-state quantum systems. This study was conducted in an advanced undergraduate quantum mechanics course, in both an online and in-person learning environment. Student learning was first assessed after receiving traditional lecture-based instruction on relevant concepts, and their performance on it was compared with that on a similar assessment given after engaging with the CQS. We analyze and discuss similar and differing trends observed in the two modes of instruction.