Development and validation of a simple and rapid thin-layer chromatography–UV densitometry method for the determination of triazophos in human whole blood for forensic toxicological applications

Sanganalmath, Praveen U.; Nagaraju, Purigali M.; Sreeramulu, K.

doi:10.1007/s00764-020-00022-3

Cited by 2 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An LOQ ≈ 1 μg/ml and recovery >90% for 1–50 μg/ml quinalphos was obtained. Another organothiophosphate, triazophos, was analyzed in a similar way using ethyl acetate as extraction solvent from whole blood adjusted to pH 5 [76]. An LOQ ≈ 2 μg/ml and recovery >90% for 1–50 μg/ml triazophos was obtained.…”

Section: Liquid‐liquid Extractionmentioning

confidence: 99%

Sample preparation for planar chromatography

Poole

2023

J of Separation Science

View full text Add to dashboard Cite

High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

show abstract

Section: Liquid‐liquid Extractionmentioning

confidence: 99%