Development and validation of a fluorescence HPLC-based screening assay for inhibition of human estrogen sulfotransferase

Reinen, Jelle; Vriese, Eveline; Glatt, Hansruedi; Vermeulen, Nico

doi:10.1016/j.ab.2006.07.015

Cited by 20 publications

(16 citation statements)

References 41 publications

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“…Since no chemically prepared pyrene 1-sulfate was available, calibration curves of pyrene 1-sulfate were made as described previously (Reinen 2006). Briefly, OHP was incubated in the presence of PAPS and hSULT1A1 with a resulting pyrene 1-sulfate yield of more than 97% in all cases (Ma 2003).…”

Section: Hplc Analysis Of 1-hydroxypyrene and Pyrene 1-sulfatementioning

confidence: 99%

“…Human SULT1E1 (hSULT1E1) and SULT1A1 (hSULT1A1) were expressed as described previously (Reinen 2006). Bacterial cytosol was prepared as described above.…”

Section: Cytosolic Preparationsmentioning

confidence: 99%

“…In order to measure the sulfonation of 1-hydroxypyrene, HPLC analysis was performed as reported previously (Reinen 2006). Extracts (50 µl) from cytosolic fractions of M a n u s c r i p t 9 mSULT1E1 incubated with OHP were analyzed for the formation of pyrene 1-sulfate by HPLC (pumps 303 and 305, manometer 805, dynamic mixer 811B and auto-injector 234, manufactured by Gilson, Middleton, USA) using a reversed phase C-18 column (ChromSpher 5 µm, 100 mm x 3 mm, Chrompack, The Netherlands) and a gradient elution with solvent A (5% ACN 10 mM ammonium acetate (pH 5)) and solvent B (90% ACN 10 mM ammonium acetate (pH 5)).…”

Section: Hplc Analysis Of 1-hydroxypyrene and Pyrene 1-sulfatementioning

confidence: 99%

“…We have recently developed a fluorescence HPLC-based screening assay for inhibition of hSULT1E1 (Reinen 2006) which makes use of the nonradioactive and non-carcinogenic substrate 1-hydroxypyrene (OHP) and hSULT1E1 expressed in Salmonella typhimurium.…”

Section: Introductionmentioning

confidence: 99%

“…This selection included 19 EDCs which were used previously for hSULT1E1 inhibition screening under almost identical conditions (Reinen 2006). The reactions were initiated by adding 50 µl of 50 µM PAPS to 150 µl of a mixture containing 10 ng mSULT1E1, 250 nM OHP, and 2.5 µM of each library compound in a 100 mM phosphate buffer (pH 7.4) containing 2 mM EDTA and 1 mM DTT.…”

mentioning

confidence: 99%

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Comparison of murine and human estrogen sulfotransferase inhibition in vitro and in silico—Implications for differences in activity, subunit dimerization and substrate inhibition

Stjernschantz

Reinen

Meinl

et al. 2010

Molecular and Cellular Endocrinology

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Section: Hplc Analysis Of 1-hydroxypyrene and Pyrene 1-sulfatementioning

confidence: 99%

“…Human SULT1E1 (hSULT1E1) and SULT1A1 (hSULT1A1) were expressed as described previously (Reinen 2006). Bacterial cytosol was prepared as described above.…”

Section: Cytosolic Preparationsmentioning

confidence: 99%

Section: Hplc Analysis Of 1-hydroxypyrene and Pyrene 1-sulfatementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Comparison of murine and human estrogen sulfotransferase inhibition in vitro and in silico—Implications for differences in activity, subunit dimerization and substrate inhibition

Stjernschantz

Reinen

Meinl

et al. 2010

Molecular and Cellular Endocrinology

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Microalgae‐promoted photodegradation of two endocrine disrupters in aqueous solutions

Deng

et al. 2008

J of Chemical Tech & Biotech

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BACKGROUND: Photodegradation is one method to remove the organic pollutants in wastewater. The photodegradation of 17α-ethynylestradiol (EE2) and 17β-estradiol (E2) was investigated using freshwater algae suspended in aqueous solutions under a high-pressure mercury lamp (250 W). Three algal species were used: Chlorella vulgaris (CV), Anabaena cylindrica (AC) and Microcytis aeruginosa kutz (MA). Reactions were carried out under aerobic conditions. RESULTS: The three algal species are capable of promoting the photodegradation of EE2 and E2. For example, in the A. cylindrica suspensions, with cell density ranging from 7.0 × 10 5 cells mL −1 to 8.4 × 10 6 cells mL −1 , the photodegradation rate of EE2 was increased from 14% to 28%. Reactive oxygen species were thought to be the main reason for the degradation of endocrine disrupting chemicals (EDCs). Hydroxyl radicals and singlet oxygen photogenerated in the algal suspensions were detected. About 4 µmol L −1 hydroxyl radicals were generated in the 4 h reaction.CONCLUSION: Oxidative photodegradation of a solution of endocrine disrupters promoted by algae leads to noticeable decrease in the toxicity of the solution. Oxygen plays an important role in the formation of reactive oxygen species in the algal suspensions.

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Analysis for estrogens as environmental pollutants – A review

Pacáková¹,

Loukotková

Bosáková

et al. 2009

J of Separation Science

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The approaches to the analysis for estrogen compounds as environmental pollutants are critically reviewed and evaluated on the basis of significant, recent original publications. The importance of sample pretreatment and analyte preconcentration techniques is pointed out, with an emphasis on SPE and on the use of highly selective interactions such as molecular recognition. The hyphenated systems of high-performance gas or liquid chromatography and mass spectrometric techniques are discussed as the basic methods of determination of estrogens in environmental samples. Immunochemical procedures are shown to be useful in semiquantitative screening of estrogen pollutants (e.g. ELISA kits). Classical HPLC and GC with common UV/Vis, fluorescence and electrochemical detection are useful in routine checking on higher pollutant concentrations.

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Development and validation of a fluorescence HPLC-based screening assay for inhibition of human estrogen sulfotransferase

Cited by 20 publications

References 41 publications

Comparison of murine and human estrogen sulfotransferase inhibition in vitro and in silico—Implications for differences in activity, subunit dimerization and substrate inhibition

Comparison of murine and human estrogen sulfotransferase inhibition in vitro and in silico—Implications for differences in activity, subunit dimerization and substrate inhibition

Microalgae‐promoted photodegradation of two endocrine disrupters in aqueous solutions

Analysis for estrogens as environmental pollutants – A review

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