Development and Validation of a UPLC-ESI-MS Method for Quantitation of the Anti-Alzheimer Drug Galantamine and other Amaryllidaceae Alkaloids in Plants

Abstract

Our study aimed at the development of a method based on ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-ESI-MS) using selected ion monitoring for the simultaneous quantitation of the alkaloids galantamine, pseudolycorine, sanguinine and narciclasine in Amaryllidaceae species (Hippeastrum elegans, Habranthus cf. irwinianus, Hymenocallis littoralis and Griffinia nocturna). The alkaloids were extracted from dried and ground bulbs (100 mg) using liquid-liquid microextraction followed by solid phase extraction in cation exchange cartridges. The quantification method showed good linearity (correlation coefficient, R ≥ 0.9968) and selectivity for a run time of 10 min. All values were within the acceptable limits for recovery (87.5-96.2%), interday (coefficient of variation, CV%, 1.3-8.4%) and intraday precision (CV% 5.7-8.1%), except narciclasine (70%). The limits of detection and quantification were 5-100 and 20-350 ng mL-1 , respectively. Our method demonstrated to be rapid, sensitive and reliable, therefore appropriate for being employed in the prospection of new source of galantamine and other alkaloids.

Comparison of Secondary Metabolite Contents and Metabolic Profiles of Six Lycoris Species

“…de Paiva et al [28] reported the analysis of four alkaloids (galantamine, sanguinine, pseudolycorine, and narciclasine) in different Amaryllidaceae plants, and galantamine was detected in all of them [28]. Our results coincide with this observation because galantamine was detected in all of the Lycoris species.…”

Chromatographic Techniques and Pharmacological Analysis as a Quality Control Strategy for Serjania triquetra a Traditional Medicinal Plant

“…Furthermore, MS analysis provides more structural information on the chemical profile than the conventional chromatographic coupled to light detectors (HPLC-UV or HPLC-DAD), allowing for more confident identification. For quantitative MS analysis, the method of choice relies on tandem systems, mainly composed of triple quadrupole detectors, since higher sensitivity is achieved by monitoring specific ion transitions (the so-called multiple reaction monitoring MRM) [ 29 , 30 ]. This approach has been successfully applied in many quality control applications.…”

Preparation and Release Profiles in Vitro/Vivo of Galantamine Pamoate Loaded Poly (Lactideco-Glycolide) (PLGA) Microspheres

“…Entrance potential (EP), collision cell exit potential (CXP), and declustering potential (DP) were set at 10, 11, and 91 V, respectively. The MRM transitions monitored in the experiments is m/z 288.2 ([M + H]) for galantamine ( Suresha et al, 2014 ; de Paiva et al, 2019 ).…”