Development and Validation of a Quantification Method for L-DOPA in Plants and Pharmaceutical Materials

Bulduk, İbrahim; Topal, Nurdoğan

doi:10.15671/hjbc.773988

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Since the absorption spectrum of LD is highly characteristic ( Figure 1 B), its determination by spectrum acquisition (LC-UV analysis) is also selective. Although several scientific works reported the separation and quantification of LD in Vicia faba L. by using liquid chromatography coupled to a UV–Vis detector (LC/UV–Vis) [ 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ], there are still some limitations regarding LD extraction/stability, chromatographic conditions, and method validation.…”

Section: Introductionmentioning

confidence: 99%

“…Mostly, C 18 stationary phases have been used due to the highly polar structure of the analyte [ 10 , 11 , 13 , 23 ]. Acetate/phosphate buffers at acidic pH values (<3) have also been used, although some papers report the use of acetate/phosphate buffers at pH of 4.00, 4.55, 4.66, or 7.00 [ 10 , 19 , 20 , 23 , 24 , 37 ]. It should be pointed out that, under highly acidic conditions, both analyte and residual silanol groups on the silica column packing support are fully protonated, so they cannot interact electrostatically, avoiding tailing, peaks broadening, and poor retention reproducibility [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans

et al. 2022

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L-Dopa (LD), a substance used medically in the treatment of Parkinson’s disease, is found in several natural products, such as Vicia faba L., also known as broad beans. Due to its low chemical stability, LD analysis in plant matrices requires an appropriate optimization of the chosen analytical method to obtain reliable results. This work proposes an HPLC-UV method, validated according to EURACHEM guidelines as regards linearity, limits of detection and quantification, precision, accuracy, and matrix effect. The LD extraction was studied by evaluating its aqueous stability over 3 months. The best chromatographic conditions were found by systematically testing several C18 stationary phases and acidic mobile phases. In addition, the assessment of the best storage treatment of Vicia faba L. broad beans able to preserve a high LD content was performed. The best LD determination conditions include sun-drying storage, extraction in HCl 0.1 M, chromatographic separation with a Discovery C18 column, 250 × 4.6 mm, 5 µm particle size, and 99% formic acid 0.2% v/v and 1% methanol as the mobile phase. The optimized method proposed here overcomes the problems linked to LD stability and separation, thus contributing to the improvement of its analytical determination.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans

et al. 2022

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Characterization and HPLC Method Validation for Determination of Dopamine Hydrochloride in Prepared Nano Particles and Pharmacokinetic Application

Neha

Mishra

Rani

et al. 2022

Analytical Chemistry Letters

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An Overview of Methods for L-Dopa Extraction and Analytical Determination in Plant Matrices

et al. 2022

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L-dopa is a precursor of dopamine used as the most effective symptomatic drug treatment for Parkinson's disease. Most of the L-dopa isolated is either synthesized chemically or from natural sources, but only some plants belonging to the Fabaceae family contain significant amounts of L-dopa. Due to its low stability, the unambiguous determination of L-dopa in plant matrices requires appropriate technologies. Several analytical methods have been developed for the determination of L-dopa in different plants. The most used for quantification of L-dopa are mainly based on capillary electrophoresis or chromatographic methods, i.e., high-performance liquid chromatography (HPLC), coupled to ultraviolet-visible or mass spectrometric detection. HPLC is most often used. This paper aims to give information on the latest developments in the chemical study of L-dopa, emphasizing the extraction, separation and characterization of this compound by chromatographic, electrochemical and spectral techniques. This study can help select the best possible strategy for determining L-dopa in plant matrices using advanced analytical methods.

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Development and Validation of a Quantification Method for L-DOPA in Plants and Pharmaceutical Materials

Cited by 3 publications

References 22 publications

Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans

Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans

Characterization and HPLC Method Validation for Determination of Dopamine Hydrochloride in Prepared Nano Particles and Pharmacokinetic Application

An Overview of Methods for L-Dopa Extraction and Analytical Determination in Plant Matrices

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