Development and validation of a column-switching high-performance liquid chromatographic method for the determination of sanfetrinem in rat and dog plasma by direct injection

Nardi, Claudio De; Ferrari, Luca; Nardin, E; Ruffo, Alberto; Braggio, Simone

doi:10.1016/s0378-4347(00)00533-8

Cited by 9 publications

(13 citation statements)

References 6 publications

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“…The major advantage of column-switching system is the direct injection of protein-rich viscous samples, such as plasma, without the need for prior treatment or an internal standard. Several factors such as columns with high efficiency stationary phases, composition of organic solvents and pHs of mobile phase were adjusted to optimize the analysis of azasetron in this study [13,18,19].…”

Section: Resultsmentioning

confidence: 99%

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Um¹,

Chae²,

Park³

et al. 2010

J of Separation Science

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For the quantification of azasetron in rat plasma samples, a column-switching HPLC method was developed and validated. Following dilution of plasma samples with mobile phase A (17 mM potassium phosphate buffer (pH 3.0)) and simple protein precipitation by addition of perchloric acid (60%), the mixture was directly injected onto the pre-column. After endogenous plasma substances were eluted to waste, the analyte was transferred to the trap column by switching the system. Then, the analyte was back-flushed to the analytical column for separation with mobile phase B (a 22:78 v/v mixture of acetonitrile and 17 mM potassium phosphate buffer (pH 3.0)) and detected at 250 nm using a photodiode array detector. A linear standard curve was obtained in the concentration range of 10-800 ng/mL with the correlation coefficient (r) of 0.9998. The intra- and inter-day precision and accuracy values for azasetron were in the ranges of 0.3-12.9% and 89.7-101.4%, respectively. The method was valid in terms of specificity, precision, and accuracy. In addition, this efficient analytical method was successfully applied to determine plasma concentrations of azasetron following oral administration of azasetron at a dose of 4.0 mg/kg to rats.

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Section: Resultsmentioning

confidence: 99%

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Um¹,

Chae²,

Park³

et al. 2010

J of Separation Science

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“…In this study, sensitivity and simplicity were taken into consideration. Several different factors were taken into account to optimize the analysis of metabolites M1 and M2: columns with high efficiency stationary phases, mobile phases at different pH values and with different percentages of organic solvent [14,17]. High organic modifier solvent contents may cause buffer precipitation which can be the cause of clogging in pretreatment cartridge and tubing.…”

Section: Methods Developmentmentioning

confidence: 99%

“…Especially, column-switching systems via a switching valve have great advantages in permitting the direct injection of protein rich samples, such as the serum [11, 12, 14 -16]. To minimize the possible introduction of analytical artifacts through excessive sample manipulation, a new analytical method has been developed and validated in our laboratory using HPLC with columnswitching [11,12,14,16,17]. The column-switching technique avoids offline procedures for sample cleanup [11,12,14,15,18].…”

Section: Introductionmentioning

confidence: 99%

“…To minimize the possible introduction of analytical artifacts through excessive sample manipulation, a new analytical method has been developed and validated in our laboratory using HPLC with columnswitching [11,12,14,16,17]. The column-switching technique avoids offline procedures for sample cleanup [11,12,14,15,18]. This method allowed serum samples to be directly injected onto a fully automated HPLC system, thereby resulting in a simple, convenient, and accurate assay even without an internal standard.…”

Section: Introductionmentioning

confidence: 99%

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Determination of the active metabolites of sibutramine in rat serum using column‐switching HPLC

Um¹,

Kim²,

Kim³

et al. 2008

J of Separation Science

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A simple and direct analysis using column-switching HPLC method was developed and validated for the quantification of active metabolites of sibutramine, N-mono-desmethyl metabolite (metabolite 1, M1) and N-di-desmethyl metabolite (metabolite 2, M2) in the serum of rats administered sibutramine HCl (5.0 mg/kg, p.o.). Rat serum was directly injected onto the precolumn without sample prepreparation step following dilution with mobile phase A, i. e., methanol-ACN-20 mM ammonium phosphate buffer (pH 6.0 with phosphoric acid) (8.3:4.5:87.2 by volume). After the endogenous serum components were eluted to waste, the system was switched and the analytes were eluted to the trap column. Active metabolites M1 and M2 were then back-flushed to the analytical column for separation with mobile phase B, i. e., methanol-ACN-20 mM ammonium phosphate buffer (pH 6.0 with phosphoric acid) (35.8:19.2:45 by volume) and detected at 223 nm. The calibration curves of active metabolites M1 and M2 were linear in the range of 0.1-1.0 microg/mL and 0.15-1.8 microg/mL. This method was fully validated and shown to be specific, accurate (10.4-10.7% error), and precise (1.97-8.79% CV). This simple and rapid analytical method using column-switching appears to be useful for the pharmacokinetic study of active metabolites (M1 and M2) of sibutramine.

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“…: +82 2 380 1774; fax: +82 2 380 1776. procedures are increasingly used [4]. And especially, columnswitching systems via a switching valve have great advantages in permitting the direct injection of protein rich samples, such as plasma [5][6][7]. In case of non-steroidal anti-inflammatory drugs (NSAIDs) used for the treatment of rheumatoids (RA), some drug substances such as naproxen, ibuprofen and ketoprofen have been reported about the bioanalytical method using column-switching technique [4,[8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma

Choi¹,

Um²,

Jung³

et al. 2006

Journal of Chromatography B

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Development and validation of a column-switching high-performance liquid chromatographic method for the determination of sanfetrinem in rat and dog plasma by direct injection

Cited by 9 publications

References 6 publications

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Determination of the active metabolites of sibutramine in rat serum using column‐switching HPLC

Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma

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